FastQCFastQC Report
Fri 17 Jun 2016
SRR1526990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68925
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5110.7413855640188611No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.4526659412404788No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.30758070366340223No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.29452303228146537No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.23213638012332247No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.19586507072905332No Hit
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.191512513601741No Hit
TGCAATGGGCAAAATTACTTCTGATCCCTCTTTCTTTATAGAGTGCAGTCATTGAACTCATGGG1120.16249546608632573No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.14798694232861806No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.13202756619513964No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.12767500906782736No Hit
TGCAATGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT880.12767500906782736No Hit
TGCAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA740.10736307580703663No Hit
TGCAATGGGATCACTGAATTCCCTATAATTTATTTTCTTTTAAAAAGATACTGTTATGTCCAGT730.10591222343126587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGC150.004540038358.40995856
AAGGGCA150.004540038358.40995857
AAAGGCC150.004540038358.40995857
CGAAGGC201.9286234E-458.40995856
ATGGGCG1000.058.2819635
GGGATAT258.339992E-658.2819637
GGGTATT150.00457968458.281967
TGGGATA452.910383E-1158.281966
TGGGACG452.910383E-1158.281966
GGGATGC303.5870835E-758.281967
CAATGGA750.058.281953
GGTTATC150.0046597558.0276378
GGAGAGT351.6050762E-858.0276348
AACGTGG150.00467319557.98546651
GGTAACG258.596404E-657.98546612
CATGCTC150.00467319557.98546615
TCATTGA258.596404E-657.98546649
AACCAGC258.596404E-657.98546611
TTACTTC258.596404E-657.98546615
GATTTGT150.00467319557.98546615
Produced by FastQC (version 0.11.5)