FastQCFastQC Report
Fri 17 Jun 2016
SRR1526989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86803
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11721.3501837494095825No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5840.6727878068730343No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.5783210257709986No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4730.5449120422105227No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.40551593838922617No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2610.3006808520442842No Hit
TGCAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2530.29146458071725634No Hit
TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC2340.26957593631556515No Hit
TGCAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2160.24883932582975243No Hit
TGCAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1820.209670172689884No Hit
TGCAATGGGGGGCATAGGGCCTTCACAGGACCAAGAGCCTCTCCTCCCATTGATATTGTCAGTA1770.20391000311049157No Hit
TGCAATGGGGAGTGCAGTGCTTGCAATGGGAGGACTGGAGAAATGGCTCAGCGGTTAAGAGCAT1740.2004539013628561No Hit
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.18547746045643584No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.1808693247929219No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.16819695171825857No Hit
TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1450.1670449178023801No Hit
TGCAATGGGGTTTCCATGCTCCCTCTCGGCCTCCTGGACCAGCATGTCCTGGAGAGCATCCCTA1400.1612847482229877No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.15782864647535222No Hit
TGCAATGGGAGCCCTGTGGCTGTTTGTCACCTGTACTGGTTGTTCTCAACCAGGGTTCATTTCC1320.15206847689595981No Hit
TGCAATGGGCAAAATTACTTCTGATCCCTCTTTCTTTATAGAGTGCAGTCATTGAACTCATGGG1290.14861237514832437No Hit
TGCAATGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT1280.14746034123244586No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.14746034123244586No Hit
TGCAATGGGGAACCAGCCCAAATGGAGCCAACAGATGAATGGATAAGGAAAACGTGGCACATAT1280.14746034123244586No Hit
TGCAATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1260.1451562734006889No Hit
TGCAATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG1260.1451562734006889No Hit
TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1200.13824406990541802No Hit
TGCAATGGGGATGTTGCCCGACTCCAGGACTTCCAGGAGCTGGAACTGGTTCTGGTGATAAGTG1180.13594000207366105No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCCCTCCCCG1170.13478796815778257No Hit
TGCAATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1100.12672373074663318No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTTCCC1080.12441966291487622No Hit
TGCAATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1020.11750745941960532No Hit
TGCAATGGGGATGTTGCCCGACTCCAGGACTTCCAGGAGCTGGAACTGGTTCTGATGATAAGTG990.11405135767196986No Hit
TGCAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT990.11405135767196986No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.1117472898402129No Hit
TGCAATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.10829118809257744No Hit
TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.10713915417669896No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.104835086344942No Hit
TGCAATGGGGCAGCGGAGCTCTCCCAAGGCCTGCAGCAAACTGGGCAGCACCTTAGCTTCCAGC890.10253101851318504No Hit
TGCAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA890.10253101851318504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATAT150.00457554758.30818658
AATATTA150.00457554758.30818658
TCTACTG150.00457554758.30818658
CTCCACG150.004607195558.20683754
ATATTGT258.422547E-658.20683753
GTACACC201.9650406E-458.20683353
TTAGCTT201.9650406E-458.20683353
GCACATA201.9650406E-458.20683357
CGTTGAT150.00461778158.17313855
AAACGTG150.00461778158.17313850
CTCGCTA150.00461778158.17313852
ATAGTTT150.00461778158.17313847
CCACGCT150.00461778158.17313856
ACCTTAG150.00461778158.17313850
CTAATAT150.00461778158.17313856
GATATTG258.4515505E-658.17313452
CCATTGA258.4515505E-658.17313447
ACAGTAC201.970681E-458.1731350
GATGCTT201.970681E-458.1731349
AGTACAC201.970681E-458.1731352
Produced by FastQC (version 0.11.5)