FastQCFastQC Report
Fri 17 Jun 2016
SRR1526988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85870
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11061.2879934785140328No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4940.5752882263887271No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4760.5543263072085711No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.5007569581926168No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.4343775474554559No Hit
TGCAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2300.26784674507977174No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2270.2643530918830791No Hit
TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC1970.22941655991615234No Hit
TGCAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1940.22592290671945964No Hit
TGCAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1730.20146733434261094No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.18865727262140444No Hit
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.186328170490276No Hit
TGCAATGGGGTTTCCATGCTCCCTCTCGGCCTCCTGGACCAGCATGTCCTGGAGAGCATCCCTA1510.17584721090019798No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.17468265983463374No Hit
TGCAATGGGGAGTGCAGTGCTTGCAATGGGAGGACTGGAGAAATGGCTCAGCGGTTAAGAGCAT1470.17118900663794107No Hit
TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1470.17118900663794107No Hit
TGCAATGGGGGGCATAGGGCCTTCACAGGACCAAGAGCCTCTCCTCCCATTGATATTGTCAGTA1360.1583789449167346No Hit
TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1350.15721439385117036No Hit
TGCAATGGGCAAAATTACTTCTGATCCCTCTTTCTTTATAGAGTGCAGTCATTGAACTCATGGG1250.14556888319552813No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.13858157680214278No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTTCCC1150.13392337253988587No Hit
TGCAATGGGGAACCAGCCCAAATGGAGCCAACAGATGAATGGATAAGGAAAACGTGGCACATAT1130.13159427040875743No Hit
TGCAATGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACAT1090.12693606614650052No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCCCTCCCCG1080.1257715150809363No Hit
TGCAATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1070.12460696401537207No Hit
TGCAATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG1060.12344241294980784No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.11994875975311517No Hit
TGCAATGGGAGCCCTGTGGCTGTTTGTCACCTGTACTGGTTGTTCTCAACCAGGGTTCATTTCC1030.11994875975311517No Hit
TGCAATGGGGATGTTGCCCGACTCCAGGACTTCCAGGAGCTGGAACTGGTTCTGGTGATAAGTG1020.11878420868755095No Hit
TGCAATGGGGATGTTGCCCGACTCCAGGACTTCCAGGAGCTGGAACTGGTTCTGATGATAAGTG1000.11645510655642251No Hit
TGCAATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG980.11412600442529405No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.11063235122860138No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC950.11063235122860138No Hit
TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.11063235122860138No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.10830324909747292No Hit
TGCAATGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGACTTAAT900.10480959590078023No Hit
TGCAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA890.10364504483521603No Hit
TGCAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT880.10248049376965179No Hit
TGCAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA870.10131594270408757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAATCA150.004511463558.51536645
CTACGGC201.9197431E-458.48080444
GATAAGG150.00452203858.480842
CTGGAAC303.513669E-758.480840
CAAGGTT150.004532631558.4462825
AGCGAGT150.004532631558.4462825
ACGAGCC150.004532631558.4462826
AATAAAC150.004532631558.4462826
GCAAGGT150.00455387458.37735724
TTACGAG150.00455387458.37735724
ATCCGCT201.942295E-458.3429620
GTAGTGT201.942295E-458.3429621
AGCTTTA150.004564522858.34295720
AAGTAAC150.004639584658.10328755
AAATCAT150.004639584658.10328746
CATATGA150.004639584658.10328749
TTTCATA150.004639584658.10328746
AAACGTG150.004639584658.10328750
TCGGAAA150.004639584658.10328754
TCTCGGA150.004639584658.10328752
Produced by FastQC (version 0.11.5)