SRR1526987_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76116
Sequences flagged as poor quality	0
Sequence length	56
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1242	1.6317200063061643	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	546	0.7173261863471544	No Hit
TGCAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	494	0.6490094066950445	No Hit
TGCAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	341	0.44800042041095167	No Hit
TGCAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	283	0.37180093541436754	No Hit
TGCAATGGGGGGCATAGGGCCTTCACAGGACCAAGAGCCTCTCCTCCCATTGATAT	206	0.2706395501602817	No Hit
TGCAATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	199	0.26144306059172845	No Hit
TGCAATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	186	0.24436386567870094	No Hit
TGCAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	184	0.24173629723054285	No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	154	0.2023227705081717	No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.1944400651636975	No Hit
TGCAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	147	0.19312628093961848	No Hit
TGCAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	140	0.18392979137106522	No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139	0.18261600714698617	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	134	0.176047086026591	No Hit
TGCAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	132	0.1734195175784329	No Hit
TGCAATGGGGAGTGCAGTGCTTGCAATGGGAGGACTGGAGAAATGGCTCAGCGGTT	119	0.15634032266540543	No Hit
TGCAATGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC	115	0.15108518576908928	No Hit
TGCAATGGGGTTTCCATGCTCCCTCTCGGCCTCCTGGACCAGCATGTCCTGGAGAG	113	0.1484576173209312	No Hit
TGCAATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	112	0.14714383309685217	No Hit
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110	0.1445162646486941	No Hit
TGCAATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	103	0.13531977508014084	No Hit
TGCAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	100	0.13137842240790373	No Hit
TGCAATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	96	0.12612328551158758	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	96	0.12612328551158758	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	95	0.12480950128750853	No Hit
TGCAATGGGGATGTTGCCCGACTCCAGGACTTCCAGGAGCTGGAACTGGTTCTGGT	95	0.12480950128750853	No Hit
TGCAATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.11955436439119238	No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.11955436439119238	No Hit
TGCAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	90	0.11824058016711335	No Hit
TGCAATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT	89	0.11692679594303432	No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.11692679594303432	No Hit
TGCAATGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTA	89	0.11692679594303432	No Hit
TGCAATGGGCAAAATTACTTCTGATCCCTCTTTCTTTATAGAGTGCAGTCATTGAA	87	0.11429922749487623	No Hit
TGCAATGGGGAACCAGCCCAAATGGAGCCAACAGATGAATGGATAAGGAAAACGTG	87	0.11429922749487623	No Hit
TGCAATGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT	87	0.11429922749487623	No Hit
TGCAATGGGGAACGAGGTTCTGGTGACCCTAGCCGCGTTCGCTCCTTAGTCCTTTC	86	0.11298544327079721	No Hit
TGCAATGGGGAGGGTGCAGCTTGCTGCTGCACAGAGTTGAAGTCTGGCCTCACTGC	85	0.11167165904671816	No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.10641652215040201	No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	78	0.1024751694781649	No Hit
TGCAATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	77	0.10116138525408586	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGTT	15	0.0078031034	50.941452	49
GTAGGAG	15	0.0078031034	50.941452	49
TAGGAGT	15	0.0078031034	50.941452	50
ATTGATA	20	3.792801E-4	50.941452	49
CATTGAT	20	3.80543E-4	50.907196	48
GAAAACG	15	0.007823902	50.907196	48
ACTGAGT	20	3.972669E-4	50.466	45
ATGTCCT	20	3.972669E-4	50.466	44
TGTTGCT	15	0.008098063	50.466	45
AATTATC	15	0.008098063	50.466	45
TCCCATT	25	1.9582287E-5	50.466	45
GTTGCTC	15	0.008098063	50.466	46
CGTACTG	25	1.9659767E-5	50.43238	42
GATAAGG	15	0.008119444	50.432377	42
TGTATCT	15	0.008119444	50.432377	42
AAATTTT	20	3.9857716E-4	50.432377	42
ACTGTTG	15	0.008119444	50.432377	42
ATAAGGA	15	0.008119444	50.432377	43
CATGTCC	20	3.9857716E-4	50.432377	43
GAAATTT	15	0.008162333	50.36527	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content