FastQCFastQC Report
Fri 17 Jun 2016
SRR1526986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1232.9560201874549383No Hit
TTCCGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA471.1295361691900985No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.7930785868781542No Hit
TTCCGCGGGAAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.5527517423696227No Hit
TTCCGCGGGTACACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.45662100456621No Hit
TTCCGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3604902667627974No Hit
TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3604902667627974No Hit
TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.26435952895938475No Hit
TTCCGCGGGAAGCACTGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.2403268445085316No Hit
TTCCGCGGGCTGGTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2403268445085316No Hit
TTCCGCGGGTGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2403268445085316No Hit
TTCCGCGGGACTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2403268445085316No Hit
TTCCGCGGGCTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2403268445085316No Hit
TTCCGCGGGAAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.2403268445085316No Hit
TTCCGCGGGAATGCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.21629416005767843No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.21629416005767843No Hit
TTCCGCGGGAGGGTTTACTCTTAAATACAATTAGAAAAAAAAAGTTGGCAAAACCTATTTTTTA80.19226147560682527No Hit
TTCCGCGGGAAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19226147560682527No Hit
TTCCGCGGGAAGCACAGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1682287911559721No Hit
TTCCGCGGGAAGGACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1682287911559721No Hit
TTCCGCGGGAAGCACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1682287911559721No Hit
TTCCGCGGGAGTGTTATCAACGCGTAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1682287911559721No Hit
TTCCGCGAGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1682287911559721No Hit
TTCCGCGGGCTATCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1682287911559721No Hit
TTCCGCGGGACTGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGCAGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGTTCAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGTACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGAAGCTGGTATCAACGCAGAGTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGAAGCATTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14419610670511895No Hit
TTCCGCGGGAAGTACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14419610670511895No Hit
TTCCGCGGGAAGCGTTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGAAGCAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT60.14419610670511895No Hit
TTCCGCGGGAAGCACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14419610670511895No Hit
TTCCGCGGAAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14419610670511895No Hit
TTCCGCGGGAAGCATGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1201634222542658No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT50.1201634222542658No Hit
TTCCGCGGGAAGCACTGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1201634222542658No Hit
TTCCGCGGGGGATATGCCCGGAGCTCCCTGCTGGCATCATGGCCTACAGAAGAGTCTCTATTCA50.1201634222542658No Hit
TTCCGCGGGAAGCACTGTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1201634222542658No Hit
TTCCGCGGGACTAAACCAAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1201634222542658No Hit
TTCCGCGGGAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1201634222542658No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1201634222542658No Hit
TTCCGCGGGCAGTGAACTTACATATAATAAATAAATAAATCTTAAAAAAAAAAAAAAAAAAAAA50.1201634222542658No Hit
TTCCGCGGGACATGTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1201634222542658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT302.7087845E-758.6219525
GCGGGAG302.7087845E-758.6219525
CGCGGGT150.00418128458.6219524
GGGTGCC201.6908273E-458.6219528
CGGGGTG256.7896053E-658.621956
GGAAGTA256.7896053E-658.621958
GGGAAGT256.7896053E-658.621957
CTCTGCC201.797968E-457.91566520
CTCTGAC150.00439080557.91566520
TCCCAAA201.797968E-457.91566521
GAGTGCA201.797968E-457.91566529
CTCCCAA201.797968E-457.91566520
GAAGCAC404.7293724E-1057.9156659
AGTGGGA150.00439080557.91566514
AGGGCAG150.00439080557.91566530
GGATCAA150.00439080557.91566518
ACTCTGC302.9550392E-757.91566519
ACTCTGA150.00439080557.91566519
ACTCCCA201.797968E-457.91566519
CACTGCA302.9550392E-757.91566513
Produced by FastQC (version 0.11.5)