##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4161
Sequences flagged as poor quality	0
Sequence length	64
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.732035568372986	39.0	38.0	39.0	35.0	39.0
2	37.82167748137467	39.0	38.0	39.0	35.0	39.0
3	37.50901225666907	39.0	38.0	39.0	35.0	39.0
4	37.642874309060325	39.0	38.0	39.0	35.0	39.0
5	36.51886565729392	38.0	35.0	39.0	31.0	39.0
6	36.87238644556597	38.0	36.0	39.0	33.0	39.0
7	37.09661139149243	38.0	36.0	39.0	33.0	39.0
8	36.73371785628455	38.0	36.0	39.0	33.0	39.0
9	36.606104301850515	38.0	35.0	39.0	33.0	39.0
10	34.54337899543379	36.0	33.0	39.0	27.0	39.0
11	35.60538332131699	38.0	35.0	39.0	30.0	39.0
12	34.696707522230234	36.0	33.0	38.0	28.0	39.0
13	34.80749819754867	36.0	33.0	38.0	28.0	39.0
14	34.59913482335977	36.0	33.0	38.0	27.0	39.0
15	34.70247536649844	36.0	33.0	38.0	28.0	39.0
16	34.06368661379476	36.0	33.0	38.0	27.0	39.0
17	34.375150204277816	36.0	33.0	38.0	27.0	39.0
18	34.323239605863975	36.0	33.0	38.0	27.0	39.0
19	32.728670992549866	35.0	31.0	38.0	23.0	39.0
20	33.84018264840183	36.0	33.0	38.0	26.0	39.0
21	33.985820716174	36.0	33.0	38.0	27.0	39.0
22	33.654169670752225	36.0	32.0	38.0	25.0	39.0
23	33.383801970680125	36.0	31.0	38.0	24.0	39.0
24	33.38764720019226	36.0	32.0	38.0	25.0	39.0
25	32.6450372506609	35.0	31.0	38.0	23.0	39.0
26	33.00456621004566	36.0	32.0	38.0	23.0	39.0
27	32.71665465032444	36.0	31.0	38.0	23.0	39.0
28	32.10310021629416	35.0	31.0	38.0	18.0	39.0
29	32.09444844989185	35.0	31.0	38.0	18.0	39.0
30	30.89209324681567	35.0	29.0	38.0	11.0	39.0
31	30.831050228310502	35.0	29.0	38.0	10.0	39.0
32	29.39413602499399	34.0	25.0	38.0	9.0	39.0
33	30.49411199230954	35.0	29.0	38.0	10.0	39.0
34	30.017543859649123	35.0	28.0	38.0	4.0	39.0
35	29.023311703917326	34.0	25.0	38.0	4.0	39.0
36	28.84114395577986	35.0	24.0	38.0	4.0	39.0
37	28.39605863975006	34.0	23.0	38.0	4.0	39.0
38	28.311944244172075	34.0	23.0	38.0	4.0	39.0
39	27.96226868541216	34.0	23.0	38.0	4.0	39.0
40	27.609709204518143	33.0	21.0	38.0	4.0	39.0
41	27.59577024753665	33.0	22.0	38.0	4.0	39.0
42	26.964431627012736	33.0	18.0	37.0	4.0	39.0
43	26.905551550108147	33.0	18.0	37.0	4.0	39.0
44	26.404950732996877	32.0	16.0	37.0	4.0	39.0
45	26.08026916606585	32.0	12.0	36.0	4.0	39.0
46	25.762076423936552	32.0	11.0	36.0	4.0	39.0
47	25.547224224945925	32.0	10.0	36.0	4.0	39.0
48	25.1521268925739	32.0	10.0	36.0	4.0	39.0
49	24.66137947608748	31.0	4.0	35.0	4.0	39.0
50	24.230233117039173	31.0	4.0	35.0	4.0	39.0
51	23.92790194664744	31.0	4.0	35.0	4.0	39.0
52	23.40879596250901	30.0	4.0	35.0	4.0	39.0
53	22.754866618601298	29.0	4.0	35.0	4.0	39.0
54	22.45181446767604	29.0	4.0	35.0	4.0	39.0
55	21.953136265320836	28.0	4.0	35.0	4.0	39.0
56	21.271088680605622	27.0	4.0	35.0	4.0	39.0
57	20.46935832732516	24.0	4.0	35.0	4.0	38.0
58	20.04397981254506	23.0	4.0	35.0	4.0	38.0
59	19.394857005527516	22.0	4.0	35.0	4.0	38.0
60	19.02138908916126	19.0	4.0	34.0	4.0	38.0
61	18.270608026916605	14.0	4.0	34.0	4.0	37.0
62	17.729632299927903	10.0	4.0	33.0	4.0	37.0
63	17.187935592405672	4.0	4.0	32.0	4.0	37.0
64	17.193943763518384	4.0	4.0	33.0	4.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.5137254901960802
1	2	1.4210084033613413
1	3	1.6969187675070003
1	4	1.5658263305322322
1	5	1.6635854341736618
1	6	2.384873949579834
1	7	2.0158263305322137
1	8	1.60546218487395
1	9	2.6570028011204343
1	10	4.867086834733897
1	11	2.688375350140049
1	12	2.3264705882352956
1	13	-1.7543417366946699
1	14	3.064145658263314
1	15	4.237675070028011
1	16	5.226750700280107
1	17	4.723809523809528
1	18	3.741456582633056
1	19	6.222408963585437
1	20	-0.3925770308123191
1	21	-6.796078431372543
1	22	2.5109243697478902
1	23	-1.1708683473389456
1	24	4.955182072829139
1	25	2.239355742296908
1	26	4.970588235294116
1	27	5.282913165266102
1	28	6.430112044817928
1	29	5.645098039215689
1	30	7.696218487394955
1	31	4.123529411764704
1	32	9.82591036414566
1	33	8.102941176470587
1	34	8.85966386554622
1	35	10.31904761904762
1	36	9.959523809523805
1	37	10.813305322128855
1	38	6.267927170868354
1	39	11.088095238095235
1	40	10.879691876750694
1	41	11.58025210084033
1	42	8.575350140056013
1	43	12.411484593837532
1	44	13.345798319327727
1	45	13.529271708683467
1	46	12.487675070028011
1	47	14.14481792717087
1	48	14.327310924369748
1	49	14.104061624649859
1	50	15.672689075630252
1	51	14.98249299719889
1	52	15.359663865546224
1	53	16.392717086834732
1	54	16.623529411764707
1	55	17.158403361344536
1	56	14.374089635854343
1	57	12.079691876750704
1	58	11.703921568627447
1	59	-14.575910364145653
1	60	-14.322689075630255
1	61	-13.718207282913163
1	62	-13.676750700280113
1	63	-12.709523809523805
1	64	-12.825350140056024
2	1	0.11372549019608158
2	2	-0.3789915966386559
2	3	0.29691876750700175
2	4	0.1658263305322336
2	5	0.8635854341736646
2	6	0.18487394957983128
2	7	0.6158263305322151
2	8	-0.19453781512604706
2	9	1.2570028011204357
2	10	1.0670868347338995
2	11	-0.9116246498599523
2	12	1.1264705882352928
2	13	2.0456582633053273
2	14	-1.5358543417366874
2	15	1.2376750700280112
2	16	0.6267507002801054
2	17	-0.07619047619046881
2	18	-1.458543417366947
2	19	1.4224089635854398
2	20	-3.19257703081232
2	21	-4.596078431372543
2	22	2.910924369747889
2	23	1.229131652661053
2	24	3.955182072829139
2	25	-4.960644257703091
2	26	-1.4294117647058826
2	27	-1.5170868347338988
2	28	1.6301120448179311
2	29	2.845098039215692
2	30	3.096218487394953
2	31	2.723529411764705
2	32	3.425910364145661
2	33	-1.2970588235294116
2	34	1.8596638655462208
2	35	4.5190476190476225
2	36	6.159523809523808
2	37	3.013305322128854
2	38	3.867927170868356
2	39	2.4880952380952337
2	40	8.079691876750697
2	41	7.180252100840331
2	42	4.375350140056014
2	43	8.81148459383753
2	44	2.345798319327727
2	45	7.529271708683467
2	46	5.287675070028012
2	47	7.3448179271708725
2	48	7.727310924369746
2	49	0.7040616246498601
2	50	2.8726890756302517
2	51	3.9824929971988894
2	52	0.7596638655462229
2	53	3.992717086834734
2	54	-2.376470588235293
2	55	0.7584033613445378
2	56	-11.225910364145657
2	57	-10.920308123249296
2	58	-9.096078431372554
2	59	-8.375910364145653
2	60	-9.322689075630255
2	61	-8.318207282913162
2	62	-7.476750700280114
2	63	-6.509523809523806
2	64	-6.625350140056025
3	1	0.26372549019608016
3	2	-1.0789915966386587
3	3	-2.0530812324929997
3	4	-0.4341736694677678
3	5	-0.8364145658263382
3	6	-3.365126050420166
3	7	0.015826330532213717
3	8	-3.39453781512605
3	9	-0.0929971988795657
3	10	-5.382913165266103
3	11	-7.311624649859951
3	12	-1.9235294117647044
3	13	-5.50434173669467
3	14	-4.185854341736686
3	15	-6.512324929971989
3	16	-12.773249299719893
3	17	-10.026190476190472
3	18	-7.258543417366944
3	19	-10.277591036414563
3	20	-6.392577030812319
3	21	-7.796078431372543
3	22	-6.73907563025211
3	23	-5.670868347338946
3	24	-14.044817927170861
3	25	-9.510644257703092
3	26	-11.029411764705884
3	27	-5.967086834733898
3	28	-4.069887955182072
3	29	-7.604901960784311
3	30	-9.553781512605045
3	31	-3.6264705882352963
3	32	-9.67408963585434
3	33	-10.647058823529413
3	34	-15.14033613445378
3	35	-9.68095238095238
3	36	-14.790476190476195
3	37	-8.186694677871145
3	38	-12.732072829131646
3	39	-9.411904761904765
3	40	-15.620308123249306
3	41	-10.16974789915967
3	42	-12.924649859943987
3	43	-8.088515406162468
3	44	-14.154201680672273
3	45	-7.220728291316533
3	46	-8.762324929971989
3	47	-14.35518207282913
3	48	-8.672689075630252
3	49	-5.895938375350141
3	50	-7.827310924369748
3	51	-6.017507002801111
3	52	-9.390336134453776
3	53	-9.607282913165268
3	54	-4.376470588235293
3	55	-6.841596638655464
3	56	-13.625910364145657
3	57	-9.170308123249296
3	58	-9.296078431372553
3	59	-7.825910364145653
3	60	-7.8226890756302545
3	61	-7.468207282913163
3	62	-7.426750700280113
3	63	-7.209523809523805
3	64	-5.075350140056024
4	1	0.5137254901960802
4	2	0.42100840336134127
4	3	0.19691876750700033
4	4	0.5658263305322322
4	5	1.6635854341736618
4	6	0.3848739495798341
4	7	0.2658263305322137
4	8	1.10546218487395
4	9	1.1570028011204343
4	10	-4.382913165266103
4	11	2.438375350140049
4	12	-3.6735294117647044
4	13	-3.75434173669467
4	14	2.314145658263314
4	15	2.4876750700280112
4	16	0.7267507002801068
4	17	2.4738095238095283
4	18	2.991456582633056
4	19	-2.277591036414563
4	20	3.607422969187681
4	21	3.9539215686274574
4	22	1.7609243697478902
4	23	4.829131652661054
4	24	4.205182072829139
4	25	2.989355742296908
4	26	4.470588235294116
4	27	4.532913165266102
4	28	5.930112044817928
4	29	4.895098039215689
4	30	-1.5537815126050454
4	31	-2.3764705882352963
4	32	-0.6740896358543402
4	33	-3.647058823529413
4	34	-1.6403361344537792
4	35	0.06904761904761969
4	36	-0.2904761904761948
4	37	0.31330532212885487
4	38	-2.9820728291316456
4	39	0.5880952380952351
4	40	1.6296918767506945
4	41	1.0802521008403296
4	42	2.3253501400560133
4	43	2.1614845938375318
4	44	0.8457983193277272
4	45	3.029271708683467
4	46	0.9876750700280112
4	47	-1.3551820728291304
4	48	0.07731092436974762
4	49	-3.1459383753501413
4	50	1.6726890756302524
4	51	-2.5175070028011106
4	52	2.3596638655462243
4	53	-0.8572829131652675
4	54	-1.3764705882352928
4	55	2.6584033613445364
4	56	5.874089635854343
4	57	2.8296918767507044
4	58	1.4539215686274467
4	59	6.674089635854347
4	60	7.6773109243697455
4	61	6.531792717086837
4	62	3.073249299719887
4	63	5.290476190476195
4	64	9.924649859943976
5	1	1.7637254901960802
5	2	1.6710084033613413
5	3	1.9469187675070003
5	4	1.8158263305322322
5	5	2.663585434173662
5	6	1.6348739495798341
5	7	2.2658263305322137
5	8	1.60546218487395
5	9	1.1570028011204343
5	10	2.8670868347338967
5	11	3.188375350140049
5	12	4.076470588235296
5	13	3.24565826330533
5	14	1.814145658263314
5	15	3.4876750700280112
5	16	0.4767507002801068
5	17	-3.7761904761904717
5	18	3.491456582633056
5	19	1.972408963585437
5	20	2.357422969187681
5	21	4.453921568627457
5	22	2.0109243697478902
5	23	5.079131652661054
5	24	4.705182072829139
5	25	2.989355742296908
5	26	3.970588235294116
5	27	3.532913165266102
5	28	0.9301120448179283
5	29	2.1450980392156893
5	30	3.4462184873949546
5	31	2.6235294117647037
5	32	-0.9240896358543402
5	33	1.352941176470587
5	34	3.3596638655462208
5	35	5.31904761904762
5	36	0.9595238095238052
5	37	3.563305322128855
5	38	-6.732072829131646
5	39	0.8380952380952351
5	40	-3.6203081232493055
5	41	-7.16974789915967
5	42	-9.424649859943987
5	43	-6.838515406162468
5	44	-6.154201680672273
5	45	-7.470728291316533
5	46	-3.7623249299719888
5	47	-3.1051820728291304
5	48	-5.422689075630252
5	49	-3.8959383753501413
5	50	-3.5773109243697476
5	51	-5.267507002801111
5	52	-3.1403361344537757
5	53	-2.1072829131652675
5	54	-5.376470588235293
5	55	-1.8415966386554636
5	56	-1.125910364145657
5	57	-5.420308123249296
5	58	0.2039215686274467
5	59	0.9240896358543473
5	60	-1.5726890756302545
5	61	2.2817927170868373
5	62	-0.9267507002801132
5	63	1.7904761904761948
5	64	2.674649859943976
6	1	0.11372549019608158
6	2	0.6210084033613441
6	3	1.6969187675070003
6	4	1.5658263305322322
6	5	0.46358543417365894
6	6	1.7848739495798327
6	7	0.4158263305322123
6	8	0.40546218487394725
6	9	0.2570028011204357
6	10	-0.5329131652661019
6	11	0.888375350140052
6	12	2.126470588235293
6	13	2.6456582633053287
6	14	1.2641456582633168
6	15	0.6376750700280098
6	16	1.4267507002801096
6	17	2.3238095238095298
6	18	0.5414565826330531
6	19	2.8224089635854384
6	20	0.8074229691876837
6	21	4.0039215686274545
6	22	1.1109243697478917
6	23	-0.5708683473389442
6	24	-3.6448179271708625
6	25	0.8393557422969096
6	26	2.3705882352941146
6	27	2.6829131652661005
6	28	-1.1698879551820731
6	29	2.2450980392156907
6	30	5.496218487394952
6	31	1.723529411764705
6	32	0.6259103641456605
6	33	0.30294117647058627
6	34	1.6596638655462215
6	35	0.519047619047619
6	36	-2.8404761904761955
6	37	-9.386694677871144
6	38	-3.932072829131645
6	39	-4.711904761904766
6	40	-3.320308123249305
6	41	-5.219747899159671
6	42	-8.624649859943986
6	43	-1.388515406162469
6	44	-5.654201680672273
6	45	-3.670728291316532
6	46	-4.712324929971988
6	47	-1.8551820728291304
6	48	0.5273109243697469
6	49	-5.29593837535014
6	50	-0.7273109243697462
6	51	-1.4175070028011092
6	52	0.7596638655462229
6	53	-3.007282913165266
6	54	-4.776470588235291
6	55	-8.641596638655464
6	56	-0.22591036414565835
6	57	0.2796918767507037
6	58	-0.09607843137255401
6	59	-0.5759103641456527
6	60	0.8773109243697448
6	61	1.2817927170868373
6	62	0.3232492997198868
6	63	-3.509523809523806
6	64	1.174649859943976
7	1	0.013725490196080159
7	2	-0.07899159663865873
7	3	-0.8030812324929997
7	4	-0.1841736694677678
7	5	-2.336414565826338
7	6	-1.6151260504201659
7	7	-3.7341736694677863
7	8	-3.89453781512605
7	9	-8.592997198879566
7	10	-3.1329131652661033
7	11	-3.061624649859951
7	12	-2.1735294117647044
7	13	-1.2543417366946699
7	14	-1.435854341736686
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118	9	0.2570028011204357
118	10	-0.5329131652661019
118	11	-0.5116246498599537
118	12	2.126470588235293
118	13	1.2456582633053301
118	14	1.4641456582633126
118	15	-2.7623249299719888
118	16	1.8267507002801082
118	17	-2.476190476190471
118	18	-7.258543417366944
118	19	-5.577591036414564
118	20	-5.9925770308123205
118	21	1.0039215686274545
118	22	1.9109243697478888
118	23	1.6291316526610515
118	24	2.955182072829139
118	25	-2.5606442577030926
118	26	-3.029411764705884
118	27	-2.717086834733898
118	28	-5.369887955182072
118	29	-2.954901960784312
118	30	-2.103781512605046
118	31	-2.8764705882352963
118	32	-6.574089635854339
118	33	-5.697058823529414
118	34	-3.540336134453778
118	35	-2.480952380952381
118	36	-0.8404761904761955
118	37	-1.9866946778711458
118	38	-4.732072829131646
118	39	-1.7119047619047656
118	40	-7.520308123249304
118	41	-2.4197478991596704
118	42	-8.424649859943987
118	43	-1.1885154061624696
118	44	0.9457983193277286
118	45	-3.2707282913165336
118	46	1.6876750700280105
118	47	-1.655182072829131
118	48	3.1273109243697483
118	49	-2.4959383753501427
118	50	0.07268907563025095
118	51	-0.4175070028011092
118	52	-6.240336134453777
118	53	-5.207282913165269
118	54	-5.576470588235292
118	55	-4.441596638655465
118	56	-3.2259103641456583
118	57	-3.7203081232492963
118	58	-1.8960784313725547
118	59	-3.3759103641456534
118	60	-0.9226890756302559
118	61	-0.31820728291316414
118	62	-0.2767507002801146
118	63	-0.9095238095238045
118	64	-1.4253501400560236
119	1	-0.8862745098039184
119	2	-0.5789915966386587
119	3	0.0969187675069989
119	4	-0.03417366946776923
119	5	0.2635854341736632
119	6	0.3848739495798341
119	7	-0.5841736694677877
119	8	-0.7945378151260485
119	9	0.6570028011204343
119	10	1.6670868347338939
119	11	-1.9116246498599523
119	12	-0.6735294117647044
119	13	-0.554341736694667
119	14	0.46414565826331255
119	15	1.437675070028014
119	16	-5.173249299719892
119	17	-3.2761904761904717
119	18	-1.258543417366944
119	19	-1.777591036414563
119	20	0.20742296918768233
119	21	1.2039215686274574
119	22	-0.2890756302521069
119	23	0.8291316526610544
119	24	-1.6448179271708625
119	25	-2.760644257703092
119	26	1.7705882352941131
119	27	2.082913165266099
119	28	2.0301120448179297
119	29	3.2450980392156907
119	30	3.296218487394956
119	31	1.1235294117647037
119	32	4.025910364145663
119	33	4.502941176470586
119	34	0.05966386554622005
119	35	4.31904761904762
119	36	3.5595238095238066
119	37	3.813305322128855
119	38	3.867927170868356
119	39	7.488095238095234
119	40	5.679691876750692
119	41	7.980252100840328
119	42	-1.2246498599439875
119	43	-0.18851540616246965
119	44	4.5457983193277265
119	45	4.329271708683468
119	46	2.4876750700280112
119	47	-2.255182072829129
119	48	-2.6726890756302524
119	49	-5.895938375350141
119	50	-2.727310924369746
119	51	-5.417507002801109
119	52	-6.840336134453775
119	53	-4.807282913165267
119	54	0.0235294117647058
119	55	0.7584033613445378
119	56	0.5740896358543424
119	57	0.2796918767507037
119	58	2.303921568627448
119	59	-0.5759103641456527
119	60	-1.5226890756302538
119	61	3.681792717086836
119	62	3.323249299719887
119	63	-3.1095238095238056
119	64	-1.4253501400560236
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	0.0
6	1.0
7	2.0
8	6.0
9	7.0
10	13.0
11	14.0
12	22.0
13	26.0
14	41.0
15	59.0
16	52.0
17	99.0
18	95.0
19	106.0
20	89.0
21	137.0
22	114.0
23	106.0
24	92.0
25	114.0
26	138.0
27	162.0
28	187.0
29	174.0
30	201.0
31	251.0
32	273.0
33	279.0
34	307.0
35	289.0
36	253.0
37	263.0
38	158.0
39	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.29039248735853	1.1076330363592584	0.40934264387189984	0.1926318324103058
8	98.02123552123551	1.303088803088803	0.4102316602316602	0.2654440154440154
9	93.51117519826964	4.806536890170632	0.8892093246815669	0.7930785868781542
10	19.298245614035086	68.58928142273491	4.902667627974044	7.209805335255948
11	17.904349915885604	63.13386205239125	10.64647921172795	8.315308819995193
12	68.66137947608748	11.415525114155251	10.57438115837539	9.348714251381878
13	17.20740206681086	11.87214611872146	18.000480653689017	52.91997116077866
14	17.015140591204037	54.794520547945204	13.266041816870944	14.924297043979811
15	43.403028118240805	16.077865897620764	15.885604422013937	24.633501562124486
16	16.43835616438356	16.79884643114636	50.324441240086514	16.43835616438356
17	53.35417167588362	14.979562394806445	14.450589083914403	17.215676845395528
18	37.298726267724106	20.644075943282864	14.035087719298245	28.022110069694783
19	15.885604422013937	31.891372266282143	36.33741888968998	15.885604422013937
20	15.885604422013937	41.28815188656573	14.15525114155251	28.67099254986782
21	16.270127373227588	18.86565729391973	48.30569574621485	16.558519586637825
22	16.029800528719058	20.355683729872627	16.991107906753182	46.623407834655126
23	17.56789233357366	39.437635183850034	23.407834655130976	19.586637827445326
24	23.023311703917326	43.30689738043739	17.35159817351598	16.318192742129295
25	19.254807692307693	21.610576923076923	19.014423076923077	40.12019230769231
26	35.60952151959606	24.356816542438086	17.961048328925223	22.072613609040634
27	14.39903846153846	28.26923076923077	19.423076923076923	37.90865384615385
28	14.43001443001443	48.02789802789803	21.09187109187109	16.450216450216452
29	33.41351936492663	29.805147943228288	20.856386817416407	15.924945874428676
30	14.529708924705318	51.190762569160455	22.10728891027183	12.1722395958624
31	29.956689124157844	32.77189605389798	25.745909528392687	11.525505293551491
32	13.405535499398317	30.56558363417569	42.623345367027675	13.405535499398317
33	24.885679903730445	30.830324909747294	27.292418772563177	16.991576413959088
34	18.006740491092923	32.715454983148774	27.034183919114106	22.2436206066442
35	13.005780346820808	47.10982658959538	28.63680154142582	11.247591522157997
36	21.179302045728036	33.044524669073404	29.025270758122744	16.750902527075812
37	12.614347616754934	33.654309099662974	42.705825710158884	11.025517573423206
38	22.075108329321136	32.08955223880597	34.68945594607607	11.145883485796821
39	12.42176215695715	32.354357246027924	35.17091959557053	20.05296100144439
40	13.126204238921002	31.88824662813102	44.55684007707129	10.428709055876686
41	10.867469879518072	30.819277108433734	46.024096385542165	12.289156626506024
42	10.797782598216438	31.863099542058325	44.805977343938295	12.533140515786936
43	11.44578313253012	30.987951807228914	36.65060240963855	20.91566265060241
44	11.563478679836184	31.582751144302577	35.72633100457721	21.127439171284028
45	20.8001928175464	31.11593154977103	36.105085562786215	11.97879006989636
46	11.812921890067503	32.35294117647059	35.51108968177435	20.32304725168756
47	21.51990349819059	32.18335343787696	35.19903498190591	11.097708082026537
48	22.63195950831526	32.9235960472403	33.887683779223906	10.556760665220535
49	21.905910735826296	31.917973462002415	33.751507840772014	12.424607961399277
50	13.220747889022919	37.80458383594693	36.45355850422195	12.521109770808202
51	13.344594594594595	40.68532818532818	34.45945945945946	11.51061776061776
52	20.173745173745175	32.673745173745175	35.641891891891895	11.51061776061776
53	14.444176513142029	32.16783216783217	35.905473836508314	17.482517482517483
54	14.361573738836592	35.62635771180304	34.829833454018825	15.18223509534154
55	17.543436293436294	32.98745173745174	35.666023166023166	13.803088803088803
56	16.895969104513636	33.14023654356746	37.195269128650736	12.768525223268162
57	21.206272617611578	32.810615199034984	33.727382388419784	12.255729794933655
58	19.98069498069498	32.31177606177606	33.108108108108105	14.599420849420849
59	14.720077220077222	34.77316602316603	36.14864864864865	14.358108108108109
60	14.820178614530533	34.829833454018825	34.54018826937002	15.809799662080618
61	15.082046332046332	32.19111969111969	37.64478764478765	15.082046332046332
62	14.115356534436602	31.053784375760525	33.99853979070333	20.832319299099538
63	15.250965250965251	36.87258687258687	32.11872586872587	15.757722007722009
64	15.95462225440502	36.39874487086652	32.29543808834178	15.351194786386676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.0
6	4.0
7	3.0
8	2.0
9	1.0
10	5.0
11	10.0
12	7.0
13	7.5
14	11.0
15	12.0
16	25.0
17	37.0
18	59.5
19	82.0
20	78.0
21	75.0
22	76.0
23	109.0
24	165.0
25	188.0
26	193.5
27	205.5
28	212.0
29	201.0
30	166.5
31	143.0
32	187.0
33	231.0
34	178.5
35	118.0
36	110.0
37	99.5
38	89.5
39	90.0
40	88.0
41	77.5
42	69.0
43	70.0
44	71.0
45	78.5
46	92.0
47	98.0
48	94.0
49	73.5
50	57.0
51	70.0
52	87.5
53	92.0
54	106.0
55	139.5
56	159.0
57	174.5
58	190.0
59	173.5
60	164.5
61	172.0
62	170.0
63	139.5
64	111.0
65	103.5
66	80.5
67	65.0
68	59.5
69	54.0
70	40.5
71	23.0
72	19.0
73	20.5
74	18.5
75	15.0
76	11.5
77	5.5
78	3.0
79	2.5
80	4.0
81	6.0
82	3.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.19226147560682527
8	0.4085556356645037
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.04806536890170632
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.02403268445085316
26	0.04806536890170632
27	0.02403268445085316
28	0.07209805335255948
29	0.09613073780341264
30	0.09613073780341264
31	0.1201634222542658
32	0.14419610670511895
33	0.14419610670511895
34	0.1682287911559721
35	0.21629416005767843
36	0.14419610670511895
37	0.1682287911559721
38	0.1682287911559721
39	0.1682287911559721
40	0.21629416005767843
41	0.26435952895938475
42	0.2883922134102379
43	0.26435952895938475
44	0.2403268445085316
45	0.2883922134102379
46	0.31242489786109107
47	0.38452295121365054
48	0.2883922134102379
49	0.38452295121365054
50	0.38452295121365054
51	0.4085556356645037
52	0.4085556356645037
53	0.3364575823119442
54	0.43258832011535686
55	0.4085556356645037
56	0.43258832011535686
57	0.38452295121365054
58	0.4085556356645037
59	0.4085556356645037
60	0.43258832011535686
61	0.4085556356645037
62	1.2496995914443643
63	0.4085556356645037
64	0.43258832011535686
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	4161.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.7017543859649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.81060154854079	74.0927661619803
2	4.9434187016081	7.978851237683249
3	1.429422275163788	3.460706560922855
4	0.4764740917212627	1.5380918048546022
5	0.29779630732578916	1.201634222542658
6	0.3275759380583681	1.5861571737563085
7	0.1786777843954735	1.0093727469358327
8	0.05955926146515784	0.38452295121365054
9	0.05955926146515784	0.43258832011535686
>10	0.38713519952352593	5.359288632540254
>50	0.0	0.0
>100	0.02977963073257892	2.9560201874549383
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	2.9560201874549383	No Hit
TTCCGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	1.1295361691900985	No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33	0.7930785868781542	No Hit
TTCCGCGGGAAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23	0.5527517423696227	No Hit
TTCCGCGGGTACACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.45662100456621	No Hit
TTCCGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.3604902667627974	No Hit
TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.3604902667627974	No Hit
TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.26435952895938475	No Hit
TTCCGCGGGAAGCACTGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.2403268445085316	No Hit
TTCCGCGGGCTGGTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.2403268445085316	No Hit
TTCCGCGGGTGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.2403268445085316	No Hit
TTCCGCGGGACTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.2403268445085316	No Hit
TTCCGCGGGCTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.2403268445085316	No Hit
TTCCGCGGGAAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.2403268445085316	No Hit
TTCCGCGGGAATGCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.21629416005767843	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.21629416005767843	No Hit
TTCCGCGGGAGGGTTTACTCTTAAATACAATTAGAAAAAAAAAGTTGGCAAAACCTATTTTTTA	8	0.19226147560682527	No Hit
TTCCGCGGGAAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.19226147560682527	No Hit
TTCCGCGGGAAGCACAGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.1682287911559721	No Hit
TTCCGCGGGAAGGACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.1682287911559721	No Hit
TTCCGCGGGAAGCACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.1682287911559721	No Hit
TTCCGCGGGAGTGTTATCAACGCGTAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1682287911559721	No Hit
TTCCGCGAGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1682287911559721	No Hit
TTCCGCGGGCTATCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1682287911559721	No Hit
TTCCGCGGGACTGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGCAGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGTTCAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGTACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGAAGCTGGTATCAACGCAGAGTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGAAGCATTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.14419610670511895	No Hit
TTCCGCGGGAAGTACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.14419610670511895	No Hit
TTCCGCGGGAAGCGTTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGAAGCAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT	6	0.14419610670511895	No Hit
TTCCGCGGGAAGCACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.14419610670511895	No Hit
TTCCGCGGAAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14419610670511895	No Hit
TTCCGCGGGAAGCATGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.1201634222542658	No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT	5	0.1201634222542658	No Hit
TTCCGCGGGAAGCACTGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.1201634222542658	No Hit
TTCCGCGGGGGATATGCCCGGAGCTCCCTGCTGGCATCATGGCCTACAGAAGAGTCTCTATTCA	5	0.1201634222542658	No Hit
TTCCGCGGGAAGCACTGTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.1201634222542658	No Hit
TTCCGCGGGACTAAACCAAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.1201634222542658	No Hit
TTCCGCGGGAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.1201634222542658	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.1201634222542658	No Hit
TTCCGCGGGCAGTGAACTTACATATAATAAATAAATAAATCTTAAAAAAAAAAAAAAAAAAAAA	5	0.1201634222542658	No Hit
TTCCGCGGGACATGTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.1201634222542658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.02403268445085316	0.0	0.0	0.0	0.0
8	0.02403268445085316	0.0	0.0	0.0	0.0
9	0.04806536890170632	0.0	0.0	0.0	0.0
10	0.14419610670511895	0.0	0.0	0.0	0.0
11	0.31242489786109107	0.0	0.0	0.0	0.0
12	0.31242489786109107	0.0	0.0	0.0	0.0
13	0.31242489786109107	0.0	0.0	0.0	0.0
14	0.5767844268204758	0.0	0.0	0.0	0.0
15	0.5767844268204758	0.0	0.0	0.0	0.0
16	0.5767844268204758	0.0	0.0	0.0	0.0
17	0.5767844268204758	0.0	0.0	0.0	0.0
18	0.5767844268204758	0.0	0.0	0.0	0.0
19	0.6729151646238885	0.0	0.0	0.0	0.0
20	0.7209805335255948	0.0	0.0	0.0	0.0
21	0.7209805335255948	0.0	0.0	0.0	0.0
22	0.7209805335255948	0.0	0.0	0.0	0.0
23	0.7930785868781542	0.0	0.0	0.0	0.0
24	0.8171112713290074	0.0	0.0	0.0	0.0
25	0.8171112713290074	0.0	0.0	0.0	0.0
26	0.8171112713290074	0.0	0.0	0.0	0.0
27	0.8171112713290074	0.0	0.0	0.0	0.0
28	0.8892093246815669	0.0	0.0	0.0	0.0
29	0.8892093246815669	0.0	0.0	0.0	0.0
30	0.9372746935832732	0.0	0.0	0.0	0.0
31	0.9372746935832732	0.0	0.0	0.0	0.0
32	0.9372746935832732	0.0	0.0	0.0	0.0
33	0.9372746935832732	0.0	0.0	0.0	0.0
34	0.9372746935832732	0.0	0.0	0.0	0.0
35	0.9853400624849795	0.0	0.0	0.0	0.0
36	0.9853400624849795	0.0	0.0	0.0	0.0
37	0.9853400624849795	0.0	0.0	0.0	0.0
38	1.0334054313866858	0.0	0.0	0.0	0.0
39	1.057438115837539	0.0	0.0	0.0	0.0
40	1.057438115837539	0.0	0.0	0.0	0.0
41	1.057438115837539	0.0	0.0	0.0	0.0
42	1.057438115837539	0.0	0.0	0.0	0.0
43	1.057438115837539	0.0	0.0	0.0	0.0
44	1.0814708002883922	0.0	0.0	0.0	0.0
45	1.0814708002883922	0.0	0.0	0.0	0.0
46	1.0814708002883922	0.0	0.0	0.0	0.0
47	1.0814708002883922	0.0	0.0	0.0	0.0
48	1.0814708002883922	0.0	0.0	0.0	0.0
49	1.1055034847392453	0.0	0.0	0.0	0.0
50	1.1055034847392453	0.0	0.0	0.0	0.0
51	1.1055034847392453	0.0	0.0	0.0	0.0
52	1.1055034847392453	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGGT	30	2.7087845E-7	58.621952	5
GCGGGAG	30	2.7087845E-7	58.621952	5
CGCGGGT	15	0.004181284	58.621952	4
GGGTGCC	20	1.6908273E-4	58.621952	8
CGGGGTG	25	6.7896053E-6	58.62195	6
GGAAGTA	25	6.7896053E-6	58.62195	8
GGGAAGT	25	6.7896053E-6	58.62195	7
CTCTGCC	20	1.797968E-4	57.915665	20
CTCTGAC	15	0.004390805	57.915665	20
TCCCAAA	20	1.797968E-4	57.915665	21
GAGTGCA	20	1.797968E-4	57.915665	29
CTCCCAA	20	1.797968E-4	57.915665	20
GAAGCAC	40	4.7293724E-10	57.915665	9
AGTGGGA	15	0.004390805	57.915665	14
AGGGCAG	15	0.004390805	57.915665	30
GGATCAA	15	0.004390805	57.915665	18
ACTCTGC	30	2.9550392E-7	57.915665	19
ACTCTGA	15	0.004390805	57.915665	19
ACTCCCA	20	1.797968E-4	57.915665	19
CACTGCA	30	2.9550392E-7	57.915665	13
>>END_MODULE