FastQCFastQC Report
Fri 17 Jun 2016
SRR1526985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1823.8485937830408123No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA621.3110594205963206No Hit
TTCCGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA561.184182702474096No Hit
TTCCGCGGGAAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.6343835906111228No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC260.5497991118629731Illumina Single End Adapter 2 (96% over 31bp)
TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.444068513427786No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.4017762740537111No Hit
TTCCGCGGGCTGGTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.4017762740537111No Hit
TTCCGCGGGAGGGTTTACTCTTAAATACAATTAGAAAAAAAAAGTTGGCAAAACCTATTTTTTA170.3594840346796363No Hit
TTCCGCGGGAAGCACTGCACTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.33833791499259885No Hit
TTCCGCGGGAAGCACTGTACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.3171917953055614No Hit
TTCCGCGGGTACACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3171917953055614No Hit
TTCCGCGGGAAGCACTGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.296045675618524No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTGCACTCTG140.296045675618524No Hit
TTCCGCGGGAAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.27489955593148657No Hit
TTCCGCGGGAGTGTTATCAACGCGTAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2326073165574117No Hit
TTCCGCGGGAAGCACTGTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2326073165574117No Hit
TTCCGCGGGTGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2326073165574117No Hit
TTCCGCGGGAAGCACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.2114611968703743No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA90.19031507718333687Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCATTATAACGCAGGGTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19031507718333687No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT90.19031507718333687No Hit
TTCCGCGGGCAGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19031507718333687No Hit
TTCCGCGGGAAGCACTGCATTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.19031507718333687No Hit
TTCCGCGGGAAGCTGGTATCAACGCAGAGTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTT90.19031507718333687No Hit
TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19031507718333687No Hit
TTCCGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19031507718333687No Hit
TTCCGCGGGAAGCACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.19031507718333687No Hit
TTCCGCGGGGTTGTTCAAGGGCGCATGGTGGATGCCTTGGCATGCTGAGCCGATGAAGGACGTG90.19031507718333687No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA90.19031507718333687Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGCCTTTAGTTCTTCCCATTTTACTTTTAGATCACTTTCTGAATCCTCAAATCGTG80.16916895749629943No Hit
TTCCGCGGGAAGCTTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.16916895749629943No Hit
TTCCGCGGGGGGAAAAAAATAAATAAATAAAACTCTGATTGCCAAAACCACGTGCCAAGAAATT80.16916895749629943No Hit
TTCCGCGGGGGCTGCCAGCTCGCGCTTACCCACAGCCAACCAAAGGAACAAAGACCGTCCACAC80.16916895749629943No Hit
TTCCGCGAGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.16916895749629943No Hit
TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC80.16916895749629943No Hit
TTCCGCGGGAATTGAGAAAAGTGGTCTGAAGCTTCTGAAATGTCATATTTTCAAGGTAAAATTT80.16916895749629943No Hit
TTCCGCGGGAAGGACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16916895749629943No Hit
TTCCGCGGAAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.16916895749629943No Hit
TTCCGCGGGAAGTACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16916895749629943No Hit
TTCCGCGGGGTGGCGGTGCGGATGTCAAAGATGAATTGTAAGTCCTTCGAGTTGCTCAAACACA80.16916895749629943No Hit
TTCCGCGGGACTGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.148022837809262No Hit
TTCCGCGGGAGGTATCAACGGCATGGTCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.148022837809262No Hit
TTCCGCGGGAGACAAGCTTGTCGTGGTGGACTTCTCTGCTACGTGGTGTGGACCTTGCAAAATG70.148022837809262No Hit
TTCCGCGGGAAGCACTGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.148022837809262No Hit
TTCCGCGGGAAGCACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.148022837809262No Hit
TTCCGCGGGGGATATGCCCGGAGCTCCCTGCTGGCATCATGGCCTACAGAAGAGTCTCTATTCA70.148022837809262No Hit
TTCCGCGGGCTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.148022837809262No Hit
TTCCGCGGGAAGCAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT70.148022837809262No Hit
TTCCGCGGGAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.148022837809262No Hit
TTCCGCGGGGCCAGGCATGGTGGCTCACGCTTATAATCCCAGCACTTTGGGAGGCCAAGGCGGG70.148022837809262No Hit
TTCCGCGGGAAGCACAGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.148022837809262No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC70.148022837809262No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTG70.148022837809262No Hit
TTCCGCAGGAAGCAGTGGTAGGGGAGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCCG60.12687671812222456No Hit
TTCCGCGGGAAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12687671812222456No Hit
TTCCGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12687671812222456No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT60.12687671812222456No Hit
TTCCGCGGGGTGACAATTTATCACAACATCTAAAAACTCACAAAAATCACGGTGATTTTAGGGA60.12687671812222456No Hit
TTCCGCGGGAAGCAGTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12687671812222456No Hit
TTCCGCGGGAAGCACTGCACTCTGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12687671812222456No Hit
TTCCGCGGGACATGTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12687671812222456No Hit
TTCCGCAAATAACAAAATACATGAGGCTGCTTGGCTCAAACACATGAGCAGGCATTCCCGCAAA60.12687671812222456No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG60.12687671812222456Illumina Single End Adapter 2 (100% over 30bp)
TTCCGCGGGAAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12687671812222456No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12687671812222456No Hit
TTCCGCGGGAAGGACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12687671812222456No Hit
TTCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCC60.12687671812222456No Hit
TTCCGCGGGACAGTTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12687671812222456No Hit
TTCCGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10573059843518715No Hit
TTCCGCGGGAAGTACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10573059843518715No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGTAGAGCTCGTATGCCGTCTTCTGC50.10573059843518715Illumina Single End Adapter 1 (96% over 30bp)
TTCCGCGGGAAGCAGGACTTCAAGATGGCGTCACTCGTGCCGCTGAAGGAGAAGAAGCTCATGG50.10573059843518715No Hit
TTCCGCGGAGGGGGGCGGACAGCTGCTTGGAGAACCACAAGTGAGGAAAAAAAGGCCCTAGACC50.10573059843518715No Hit
TTCCGCGGGAAGCAGTGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAA50.10573059843518715No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA50.10573059843518715Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCAGTGGTGGTTAGACAGCTCCCCCATAAGGATGCGTAGACAATTTCTCAGCT50.10573059843518715No Hit
TTCCGCGGCCTGGTGCCCATGGAACACTGCATAACTCGCTTCTTTGAGGAGTGTGACCCCAACA50.10573059843518715No Hit
TTCCGCGGGGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAG50.10573059843518715No Hit
TTCCGCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA50.10573059843518715Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGACTAAACCAAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10573059843518715No Hit
TTCCGCGGGAAGTGGTTCAACGCAGAGTCGACTCTTTTTTTTCCTTTTTTTTTTTTTTTTTTTT50.10573059843518715No Hit
TTCCGCGGGAAGCACTGCACTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10573059843518715No Hit
TTCCGCGGGGCTGCCCTCGGGATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGG50.10573059843518715No Hit
TTCCGCGGGAAGCTCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10573059843518715No Hit
TTCCGCGGGAATCGGGGTGGGGGTAGGAGGAAAAATAGAGGTGACACACAGTTCCAAGAGAACA50.10573059843518715No Hit
TTCCGCGGGAGATGGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10573059843518715No Hit
TTCCGCGGTTCTGGAAATGTCTGTCAGTTTGCAGGAAACTGGAGTGTGGGTTCGCAGCCCTCTC50.10573059843518715No Hit
TTCCGCGGGAATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10573059843518715No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG50.10573059843518715Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG302.8910654E-758.3333365
GCGGGGC150.004300905458.3333365
GCGGGAT150.004300905458.3333365
CGGGGGG150.004300905458.3333366
CGGGAAC150.004300905458.3333366
GGAAGCC150.004300905458.3333368
CGCGGGC302.8910654E-758.3333364
CGCGGGT257.14656E-658.3333324
GCGGGCA201.7579758E-458.333335
GCGGGAG201.7579758E-458.333335
CGGAAAG201.7579758E-458.333336
CGCGGCC201.7579758E-458.333334
CGCGGAA201.7579758E-458.333334
TCCGCGA201.7579758E-458.333332
CCGCGGC201.7579758E-458.333333
CTCCCAA303.1206582E-757.7127720
ACTCCCA303.1206582E-757.7127719
CACTCCC303.1206582E-757.7127718
GCACTCC303.1206582E-757.7127717
TGCACTC405.12955E-1057.71276516
Produced by FastQC (version 0.11.5)