FastQCFastQC Report
Fri 17 Jun 2016
SRR1526984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4747
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1944.086791657889193No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA581.2218243100905837No Hit
TTCCGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.9058352643775016No Hit
TTCCGCGGGAAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.7794396460922688No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC220.4634506003791869Illumina Single End Adapter 2 (96% over 31bp)
TTCCGCGGGAAGCACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.33705498209395407No Hit
TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.31598904571308195No Hit
TTCCGCGGGAAGCACTGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.2949231093322098No Hit
TTCCGCGGGTACACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.2949231093322098No Hit
TTCCGCGGGAAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.27385717295133766No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT120.25279123657046554No Hit
TTCCGCGGGAGGGTTTACTCTTAAATACAATTAGAAAAAAAAAGTTGGCAAAACCTATTTTTTA120.25279123657046554No Hit
TTCCGCGGGAAGCATTATAACGCAGGGTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.25279123657046554No Hit
TTCCGCGGGAAGCACTGTACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.25279123657046554No Hit
TTCCGCGGGAGTGTTATCAACGCGTAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.25279123657046554No Hit
TTCCGCGGGAAGCACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.23172530018959345No Hit
TTCCGCGGGGTGGCGGTGCGGATGTCAAAGATGAATTGTAAGTCCTTCGAGTTGCTCAAACACA110.23172530018959345No Hit
TTCCGCGGGAGGTATCAACGGCATGGTCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT110.23172530018959345No Hit
TTCCGCGGGGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA110.23172530018959345No Hit
TTCCGCGGGAATTGAGAAAAGTGGTCTGAAGCTTCTGAAATGTCATATTTTCAAGGTAAAATTT110.23172530018959345No Hit
TTCCGCGGGAAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.23172530018959345No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA100.21065936380872127Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGTGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.21065936380872127No Hit
TTCCGCGAGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.21065936380872127No Hit
TTCCGCGGGAAGCACTGCACTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.18959342742784915No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.18959342742784915No Hit
TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.18959342742784915No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA90.18959342742784915Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.16852749104697703No Hit
TTCCGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16852749104697703No Hit
TTCCGCGGGAAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16852749104697703No Hit
TTCCGCGGGCTGGTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.16852749104697703No Hit
TTCCGCGGGAAGCAGTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16852749104697703No Hit
TTCCGCGGGAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1474615546661049No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1474615546661049No Hit
TTCCGCGGGAAGTACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1474615546661049No Hit
TTCCGCGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTATTAGCT70.1474615546661049No Hit
TTCCGCGGGAAGCACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1474615546661049No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC70.1474615546661049No Hit
TTCCGCGGGAAGCAGTGGTATCAAGTTTTTATTCAGTGGGTCTCTGTGTCTAGAAGAAGGTGTT70.1474615546661049No Hit
TTCCGCGGGGTTGTTCAAGGGCGCATGGTGGATGCCTTGGCATGCTGAGCCGATGAAGGACGTG70.1474615546661049No Hit
TTCCGCGGGGTTTTGTTTTTTCCTTCCAACAAAGACTCTTGAAAAATCAAGACAATACGCTCTT60.12639561828523277No Hit
TTCCGCGGGAGACAAGCTTGTCGTGGTGGACTTCTCTGCTACGTGGTGTGGACCTTGCAAAATG60.12639561828523277No Hit
TTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12639561828523277No Hit
TTCCGCGGCCTGGTGCCCATGGAACACTGCATAACTCGCTTCTTTGAGGAGTGTGACCCCAACA60.12639561828523277No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT60.12639561828523277Illumina Single End Adapter 2 (96% over 33bp)
TTCCGCGGGAAGCACTGCATTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12639561828523277No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTGCACTCTG60.12639561828523277No Hit
TTCCGCGGGAAGGACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12639561828523277No Hit
TTCCGCGGGGATGAGAGACTCAAATGAATTGACGGATCGTAGTGGTCCTGAGTCGGAGACACGC60.12639561828523277No Hit
TTCCGCGGGAAGCATTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12639561828523277No Hit
TTCCGCGGGAAGCATGTTACCATGGTGGTATTCAGTACTGGTAGCCTGGCATGGTGGGTACTAC60.12639561828523277No Hit
TTCCGCGGGACTCACCCCACAAAAGACACAAAGAGAATCGCACAGCACACAGCAGGCGCAAAAA60.12639561828523277No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG60.12639561828523277Illumina Single End Adapter 2 (100% over 30bp)
TTCCGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12639561828523277No Hit
TTCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCC60.12639561828523277No Hit
TTCCGCGGGACTGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGAAGCACTGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10532968190436064No Hit
TTCCGCGGGAAGCACTGCACTCTGACAAAATATCATAAATAACACAAATGAGAAATTTTCAAGT50.10532968190436064No Hit
TTCCGCGGGAAGCACTGTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10532968190436064No Hit
TTCCGCGGGACTGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGGTCTGCTCACAGAAATCCATGGGGGTGCATGCAGCACCAGCAGCGGGAGGGCTGA50.10532968190436064No Hit
TTCCGCGGGAAGCTGGTATCAACGCAGAGTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGTCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGGGGGGTTTACGCGGGGAAGTGAAACATCTCAGTACCCGTAGGAGGAGAAAATAAA50.10532968190436064No Hit
TTCCGCGGGACTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA50.10532968190436064No Hit
TTCCGCGGGGTGACAATTTATCACAACATCTAAAAACTCACAAAAATCACGGTGATTTTAGGGA50.10532968190436064No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGTCTTCT50.10532968190436064Illumina Single End Adapter 2 (96% over 29bp)
TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC50.10532968190436064No Hit
TTCCGCGGGAGCTGGTTCAACGCAGAGTCGACTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGAGGGTATTTCAAGAATATAAATACATAGAAATGGTGCATCTTAACATTTGTTTGTA50.10532968190436064No Hit
TTCCGCGGGAAGCAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT50.10532968190436064No Hit
TTCCGCGGGACATGTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCAAATAACAAAATACATGAGGCTGCTTGGCTCAAACACATGAGCAGGCATTCCCGCAAA50.10532968190436064No Hit
TTCCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGC50.10532968190436064No Hit
TTCCGCTAGGGGGGGAAAATATTTATATGCAGGTTTAGCTGGTCCATTCTCCACGCTGTAAATA50.10532968190436064No Hit
TTCCGCGGGAAGCTCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10532968190436064No Hit
TTCCGCGGGGAGATTTAGAGCGGTTAAAACTGGAGATCGAATCTGGATTGAGAATCAGCACTGG50.10532968190436064No Hit
TTCCGCGGTTCTGGAAATGTCTGTCAGTTTGCAGGAAACTGGAGTGTGGGTTCGCAGCCCTCTC50.10532968190436064No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.10532968190436064No Hit
TTCCGCGGGAAGCACTGCATTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10532968190436064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAAA150.00423867658.5483821
CTCCCAA150.00423867658.5483820
CGGGGTG351.2334567E-857.9255336
AAGCAGT850.057.92553310
GCGGGGT500.057.925535
GCGGGGG452.0008883E-1157.925535
GCGGGCA201.8221354E-457.925535
GCGGGAG452.0008883E-1157.925535
GCGGGAC201.8221354E-457.925535
GGAGACA150.00442511357.9255355
CGGAGAC150.00442511357.9255354
CGGGGGG150.00442511357.925536
CGGGGGC150.00442511357.925536
CGGGGAT150.00442511357.925536
GAAGCAG900.057.925539
ACTCCCA150.00442511357.9255319
TCGGAGA150.00442511357.9255353
GGGAGTG201.8221354E-457.925537
CGGGAGT201.8221354E-457.925536
CGGGAAA257.463137E-657.925536