##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526984_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4747 Sequences flagged as poor quality 0 Sequence length 64 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.37392037076048 39.0 38.0 40.0 36.0 40.0 2 38.2824942068675 39.0 38.0 40.0 36.0 40.0 3 38.14998946703181 39.0 38.0 40.0 35.0 40.0 4 38.233410575100066 39.0 38.0 40.0 36.0 40.0 5 37.64314303770803 39.0 38.0 40.0 33.0 40.0 6 37.783231514640825 39.0 38.0 40.0 35.0 40.0 7 38.17758584369075 39.0 38.0 40.0 35.0 40.0 8 37.99115230672003 39.0 38.0 40.0 35.0 40.0 9 37.90857383610702 39.0 38.0 40.0 35.0 40.0 10 37.07499473351591 39.0 36.0 40.0 32.0 40.0 11 37.201811670528755 39.0 37.0 40.0 33.0 40.0 12 36.90646724246893 39.0 36.0 39.0 32.0 40.0 13 36.67579523909838 38.0 36.0 39.0 32.0 40.0 14 36.54645038971982 38.0 36.0 39.0 31.0 40.0 15 36.63450600379187 38.0 36.0 39.0 31.0 40.0 16 36.346955972192966 38.0 35.0 39.0 31.0 40.0 17 36.54855698335791 38.0 36.0 39.0 31.0 40.0 18 36.466821150200126 38.0 36.0 39.0 31.0 40.0 19 35.638297872340424 38.0 34.0 39.0 29.0 40.0 20 36.165367600589846 38.0 35.0 39.0 31.0 40.0 21 36.101327153991996 38.0 36.0 39.0 30.0 40.0 22 35.875289656625235 38.0 35.0 39.0 29.0 40.0 23 35.76111228144091 38.0 35.0 39.0 29.0 40.0 24 35.72171898040868 38.0 35.0 39.0 29.0 40.0 25 35.44638719191068 38.0 35.0 39.0 29.0 40.0 26 35.48894038340004 39.0 35.0 40.0 29.0 40.0 27 35.83716031177586 39.0 36.0 39.0 30.0 40.0 28 35.33705498209395 38.0 35.0 39.0 28.0 40.0 29 35.41352433115652 38.0 35.0 39.0 29.0 40.0 30 35.009479671371395 38.0 35.0 39.0 27.0 40.0 31 34.845586686328204 39.0 35.0 39.0 27.0 40.0 32 34.259742995576154 38.0 33.0 39.0 24.0 40.0 33 34.631346113334736 39.0 35.0 39.0 25.0 40.0 34 34.49547082367811 38.0 34.0 39.0 24.0 40.0 35 33.88434800926901 38.0 33.0 39.0 21.0 40.0 36 33.68401095428692 38.0 34.0 39.0 16.0 40.0 37 33.547714345902676 38.0 34.0 39.0 15.0 40.0 38 33.425110596166 38.0 33.0 39.0 14.0 40.0 39 33.32209816726353 38.0 33.0 39.0 10.0 40.0 40 32.99599747208764 38.0 33.0 39.0 4.0 40.0 41 32.624815673056666 38.0 33.0 39.0 4.0 40.0 42 32.41520960606699 38.0 32.0 39.0 4.0 40.0 43 32.299557615336 38.0 32.0 39.0 4.0 40.0 44 32.061301874868334 38.0 32.0 39.0 4.0 40.0 45 31.469770381293447 38.0 31.0 39.0 4.0 40.0 46 31.1643143037708 37.0 31.0 39.0 4.0 40.0 47 31.072045502422583 37.0 30.0 39.0 4.0 40.0 48 30.856119654518643 37.0 30.0 39.0 4.0 40.0 49 30.69222666947546 36.0 30.0 39.0 4.0 40.0 50 30.393301032230884 36.0 29.0 39.0 4.0 40.0 51 29.74489151042764 36.0 28.0 39.0 4.0 40.0 52 29.48936170212766 36.0 27.0 39.0 4.0 40.0 53 29.19949441752686 35.0 26.0 39.0 4.0 40.0 54 28.748683378976196 35.0 23.0 39.0 4.0 40.0 55 28.47461554666105 35.0 21.0 39.0 4.0 40.0 56 27.706130187486835 35.0 4.0 39.0 4.0 40.0 57 27.40046345060038 35.0 4.0 39.0 4.0 40.0 58 27.188329471244998 35.0 4.0 39.0 4.0 40.0 59 26.72677480514009 35.0 4.0 39.0 4.0 40.0 60 26.411628396882243 34.0 4.0 39.0 4.0 40.0 61 25.747208763429533 34.0 4.0 39.0 4.0 40.0 62 25.58036654729303 34.0 4.0 39.0 4.0 40.0 63 24.99494417526859 33.0 4.0 39.0 4.0 39.0 64 24.824942068674954 33.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -4.022689075630254 1 2 -6.59901960784314 1 3 -17.400000000000006 1 4 -12.016806722689068 1 5 -10.024789915966394 1 6 -11.528571428571418 1 7 -8.495518207282906 1 8 -8.36694677871148 1 9 -8.294397759103646 1 10 -2.9868347338935592 1 11 -1.9077030812324836 1 12 -3.2684873949579867 1 13 -0.11820728291315419 1 14 -7.408683473389338 1 15 -6.608823529411758 1 16 -5.003361344537829 1 17 -2.7245098039215634 1 18 -2.743977591036419 1 19 -1.9203081232492991 1 20 -2.3264705882352814 1 21 -4.055322128851536 1 22 -5.559243697478998 1 23 -2.8522408963585377 1 24 -0.8180672268907614 1 25 -4.686974789915965 1 26 1.8180672268907543 1 27 -5.51134453781512 1 28 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-0.8815126050420119 119 34 -0.0708683473389371 119 35 -3.879551820728288 119 36 1.525770308123242 119 37 2.4770308123249336 119 38 2.553641456582639 119 39 2.609243697479002 119 40 3.712324929972006 119 41 0.38613445378150857 119 42 -4.025350140056027 119 43 -0.5259103641456662 119 44 2.1941176470588246 119 45 1.9507002801120379 119 46 4.85770308123249 119 47 4.670028011204476 119 48 4.629411764705878 119 49 4.4441176470588175 119 50 5.251540616246487 119 51 6.954761904761895 119 52 7.262605042016816 119 53 7.682212885154062 119 54 8.120028011204479 119 55 8.243837535013999 119 56 9.25756302521009 119 57 8.71036414565826 119 58 8.78487394957984 119 59 9.606582633053218 119 60 9.50224089635855 119 61 10.781512605042014 119 62 10.870028011204486 119 63 10.952801120448182 119 64 8.638375350140056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 1.0 6 1.0 7 2.0 8 9.0 9 10.0 10 16.0 11 20.0 12 13.0 13 18.0 14 26.0 15 22.0 16 30.0 17 60.0 18 51.0 19 47.0 20 64.0 21 69.0 22 47.0 23 71.0 24 63.0 25 80.0 26 80.0 27 79.0 28 89.0 29 113.0 30 147.0 31 148.0 32 178.0 33 233.0 34 232.0 35 286.0 36 377.0 37 528.0 38 860.0 39 668.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.37792289867284 0.969033073520118 0.4634506003791869 0.18959342742784915 8 97.76371308016878 1.7088607594936709 0.31645569620253167 0.21097046413502107 9 93.23783442174005 5.287550031598904 0.7794396460922688 0.6951759005687803 10 22.730145354961028 65.97851274489152 5.877396250263324 5.413945649884138 11 20.728881398778178 59.57446808510638 10.954286918053507 8.742363598061933 12 67.24246892774384 11.691594691384031 11.502001263956183 9.563935116915948 13 18.264166842216138 11.923319991573624 19.38066147040236 50.431851695807886 14 17.274067832315147 52.180324415420266 15.63092479460712 14.914682957657469 15 40.04634506003792 16.473562249842004 16.43143037708026 27.048662313039816 16 17.67432062355172 17.210870023172532 48.99936802190857 16.11544133136718 17 50.47398356856962 17.02127659574468 14.55656203918264 17.948177796503053 18 35.26437750157994 20.012639561828525 15.757320412892353 28.965662523699176 19 17.02127659574468 33.22098167263535 33.937223509585 15.820518222034968 20 17.10554034126817 38.571729513376866 15.062144512323572 29.26058563303139 21 17.042342532125552 18.348430587739625 47.1666315567727 17.442595323362124 22 17.463661259742995 20.265430798398988 17.168738150410785 45.102169791447224 23 19.085738361070153 36.31767432062355 24.499684010954287 20.09690330735201 24 24.984200547714348 40.383400042131875 17.147672214029914 17.484727196123867 25 20.712178676780447 22.798145806995365 18.857985672144963 37.631689844079226 26 31.76194539249147 24.445392491467576 18.85665529010239 24.936006825938566 27 15.295358649789028 30.759493670886073 19.029535864978904 34.91561181434599 28 16.666666666666664 46.60337552742616 20.35864978902954 16.371308016877638 29 29.51476793248945 34.11392405063291 21.244725738396625 15.126582278481013 30 14.764817549040288 47.90128664838642 22.062855937565914 15.271039865007383 31 25.94469073253114 35.65547815072831 25.54359299134473 12.85623812539582 32 15.441487114490918 32.762991128010135 38.21292775665399 13.582594000844953 33 20.18174133558749 33.60101437024514 27.13440405748098 19.08284023668639 34 20.710059171597635 34.86897717666949 27.176669484361792 17.24429416737109 35 15.436667371537323 45.97166419961937 27.172763797843096 11.418904631000212 36 16.68076109936575 36.27906976744186 28.18181818181818 18.858350951374206 37 13.575808839078029 37.02685557200254 36.540494819200674 12.856840769718755 38 16.79001903150772 35.060266441108055 34.002960456756185 14.14675407062804 39 14.593908629441623 35.53299492385787 34.30626057529611 15.5668358714044 40 15.210492913052676 35.244341019674216 37.65601861645864 11.88914745081447 41 12.494705633206268 36.255823803473106 38.31003811944092 12.939432443879712 42 13.934773401101227 36.34053367217281 36.78526048284625 12.939432443879712 43 13.601694915254237 35.38135593220339 36.059322033898304 14.957627118644067 44 14.146548072850488 36.615840745446846 34.07454468445574 15.16306649724693 45 17.73745997865528 35.944503735325505 34.00213447171825 12.31590181430096 46 13.828886065226598 37.335874629394326 33.33333333333333 15.501905972045742 47 14.588185475333473 36.88333686216388 35.507092949396565 13.021384713106077 48 16.43371452774248 37.16645489199492 33.672172808132146 12.727657772130454 49 18.301207371319634 36.51768693073502 32.83202711289981 12.349078585045541 50 13.728813559322035 38.771186440677965 35.063559322033896 12.436440677966102 51 14.249363867684478 40.839694656488554 33.33333333333333 11.577608142493638 52 16.17896522476675 36.55640373197625 35.17811704834606 12.086513994910941 53 15.518337926648293 36.76065295738817 34.42866228535086 13.292346830612678 54 13.649851632047477 37.24035608308605 33.4463755828741 15.66341670199237 55 15.263938944244224 35.82785668857325 34.36506253974984 14.54314182743269 56 15.988125530110263 34.605597964376585 34.86005089058524 14.546225614927904 57 15.567338282078472 37.79427359490986 32.89501590668081 13.74337221633086 58 16.440390326686465 36.74162070428511 32.1383114128129 14.679677556215529 59 14.516129032258066 36.43887945670628 35.44142614601019 13.603565365025466 60 15.258913412563668 36.56621392190153 33.42529711375212 14.749575551782682 61 14.883227176220807 35.43524416135881 34.309978768577494 15.371549893842888 62 16.578221184461896 34.6847803014222 33.96306516663129 14.773933347484611 63 15.583864118895965 36.7728237791932 31.613588110403395 16.02972399150743 64 16.840093438097263 36.88681248672754 31.25929071989807 15.013803355277128 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 3.0 6 6.0 7 3.0 8 0.0 9 0.5 10 6.5 11 12.0 12 9.0 13 14.0 14 22.0 15 21.5 16 36.5 17 52.0 18 63.5 19 75.0 20 74.5 21 67.0 22 60.0 23 116.0 24 213.5 25 255.0 26 264.0 27 231.5 28 190.0 29 182.0 30 144.0 31 114.0 32 220.0 33 326.0 34 241.5 35 138.0 36 119.0 37 114.5 38 104.0 39 98.0 40 86.0 41 91.0 42 108.0 43 106.0 44 104.0 45 109.0 46 101.0 47 88.0 48 101.0 49 104.0 50 94.0 51 109.5 52 132.0 53 139.0 54 171.0 55 211.0 56 219.0 57 224.0 58 229.0 59 202.0 60 155.0 61 135.0 62 136.0 63 128.0 64 119.0 65 85.5 66 43.5 67 35.0 68 47.5 69 60.0 70 48.5 71 30.0 72 23.0 73 22.0 74 12.5 75 4.0 76 5.0 77 5.5 78 5.0 79 5.5 80 4.5 81 3.0 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.1474615546661049 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.02106593638087213 26 1.2428902464714557 27 0.1474615546661049 28 0.1474615546661049 29 0.1474615546661049 30 0.12639561828523277 31 0.21065936380872127 32 0.27385717295133766 33 0.31598904571308195 34 0.31598904571308195 35 0.3791868548556983 36 0.3581209184748262 37 0.3791868548556983 38 0.3791868548556983 39 0.4002527912365704 40 0.42131872761744255 41 0.5266484095218033 42 0.5266484095218033 43 0.5687802822835475 44 0.5266484095218033 45 1.3060880556140722 46 0.5266484095218033 47 0.5055824731409311 48 0.5266484095218033 49 0.5477143459026753 50 0.5687802822835475 51 0.6530440278070361 52 0.6530440278070361 53 0.6319780914261639 54 0.6109121550452918 55 0.6319780914261639 56 0.6530440278070361 57 0.6741099641879081 58 0.6951759005687803 59 0.7373077733305246 60 0.7373077733305246 61 0.7794396460922688 62 0.7583737097113966 63 0.7794396460922688 64 0.8005055824731409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 4747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.65262270907942 #Duplication Level Percentage of deduplicated Percentage of total 1 81.73198482932996 54.47651148093533 2 10.145385587863464 13.524331156519908 3 3.697850821744627 7.394143669686118 4 1.7699115044247788 4.718769749315356 5 0.8849557522123894 2.949231093322098 6 0.47408343868520864 1.8959342742784917 7 0.2528445006321113 1.179692437328839 8 0.15802781289506954 0.8426374552348851 9 0.12642225031605564 0.7583737097113966 >10 0.695322376738306 6.951759005687803 >50 0.03160556257901391 1.2218243100905837 >100 0.03160556257901391 4.086791657889193 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 4.086791657889193 No Hit TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 1.2218243100905837 No Hit TTCCGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 0.9058352643775016 No Hit TTCCGCGGGAAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.7794396460922688 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC 22 0.4634506003791869 Illumina Single End Adapter 2 (96% over 31bp) TTCCGCGGGAAGCACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.33705498209395407 No Hit TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.31598904571308195 No Hit TTCCGCGGGAAGCACTGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.2949231093322098 No Hit TTCCGCGGGTACACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.2949231093322098 No Hit TTCCGCGGGAAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.27385717295133766 No Hit TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 12 0.25279123657046554 No Hit TTCCGCGGGAGGGTTTACTCTTAAATACAATTAGAAAAAAAAAGTTGGCAAAACCTATTTTTTA 12 0.25279123657046554 No Hit TTCCGCGGGAAGCATTATAACGCAGGGTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.25279123657046554 No Hit TTCCGCGGGAAGCACTGTACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.25279123657046554 No Hit TTCCGCGGGAGTGTTATCAACGCGTAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.25279123657046554 No Hit TTCCGCGGGAAGCACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.23172530018959345 No Hit TTCCGCGGGGTGGCGGTGCGGATGTCAAAGATGAATTGTAAGTCCTTCGAGTTGCTCAAACACA 11 0.23172530018959345 No Hit TTCCGCGGGAGGTATCAACGGCATGGTCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.23172530018959345 No Hit TTCCGCGGGGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA 11 0.23172530018959345 No Hit TTCCGCGGGAATTGAGAAAAGTGGTCTGAAGCTTCTGAAATGTCATATTTTCAAGGTAAAATTT 11 0.23172530018959345 No Hit TTCCGCGGGAAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.23172530018959345 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA 10 0.21065936380872127 Illumina Single End Adapter 2 (97% over 34bp) TTCCGCGGGTGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.21065936380872127 No Hit TTCCGCGAGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.21065936380872127 No Hit TTCCGCGGGAAGCACTGCACTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.18959342742784915 No Hit TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.18959342742784915 No Hit TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.18959342742784915 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA 9 0.18959342742784915 Illumina Single End Adapter 2 (100% over 34bp) TTCCGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.16852749104697703 No Hit TTCCGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.16852749104697703 No Hit TTCCGCGGGAAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.16852749104697703 No Hit TTCCGCGGGCTGGTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.16852749104697703 No Hit TTCCGCGGGAAGCAGTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.16852749104697703 No Hit TTCCGCGGGAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.1474615546661049 No Hit TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.1474615546661049 No Hit TTCCGCGGGAAGTACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.1474615546661049 No Hit TTCCGCGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTATTAGCT 7 0.1474615546661049 No Hit TTCCGCGGGAAGCACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.1474615546661049 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC 7 0.1474615546661049 No Hit TTCCGCGGGAAGCAGTGGTATCAAGTTTTTATTCAGTGGGTCTCTGTGTCTAGAAGAAGGTGTT 7 0.1474615546661049 No Hit TTCCGCGGGGTTGTTCAAGGGCGCATGGTGGATGCCTTGGCATGCTGAGCCGATGAAGGACGTG 7 0.1474615546661049 No Hit TTCCGCGGGGTTTTGTTTTTTCCTTCCAACAAAGACTCTTGAAAAATCAAGACAATACGCTCTT 6 0.12639561828523277 No Hit TTCCGCGGGAGACAAGCTTGTCGTGGTGGACTTCTCTGCTACGTGGTGTGGACCTTGCAAAATG 6 0.12639561828523277 No Hit TTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.12639561828523277 No Hit TTCCGCGGCCTGGTGCCCATGGAACACTGCATAACTCGCTTCTTTGAGGAGTGTGACCCCAACA 6 0.12639561828523277 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT 6 0.12639561828523277 Illumina Single End Adapter 2 (96% over 33bp) TTCCGCGGGAAGCACTGCATTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.12639561828523277 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTGCACTCTG 6 0.12639561828523277 No Hit TTCCGCGGGAAGGACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.12639561828523277 No Hit TTCCGCGGGGATGAGAGACTCAAATGAATTGACGGATCGTAGTGGTCCTGAGTCGGAGACACGC 6 0.12639561828523277 No Hit TTCCGCGGGAAGCATTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.12639561828523277 No Hit TTCCGCGGGAAGCATGTTACCATGGTGGTATTCAGTACTGGTAGCCTGGCATGGTGGGTACTAC 6 0.12639561828523277 No Hit TTCCGCGGGACTCACCCCACAAAAGACACAAAGAGAATCGCACAGCACACAGCAGGCGCAAAAA 6 0.12639561828523277 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG 6 0.12639561828523277 Illumina Single End Adapter 2 (100% over 30bp) TTCCGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.12639561828523277 No Hit TTCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCC 6 0.12639561828523277 No Hit TTCCGCGGGACTGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGAAGCACTGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.10532968190436064 No Hit TTCCGCGGGAAGCACTGCACTCTGACAAAATATCATAAATAACACAAATGAGAAATTTTCAAGT 5 0.10532968190436064 No Hit TTCCGCGGGAAGCACTGTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.10532968190436064 No Hit TTCCGCGGGACTGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGGTCTGCTCACAGAAATCCATGGGGGTGCATGCAGCACCAGCAGCGGGAGGGCTGA 5 0.10532968190436064 No Hit TTCCGCGGGAAGCTGGTATCAACGCAGAGTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGTCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGGGGGGTTTACGCGGGGAAGTGAAACATCTCAGTACCCGTAGGAGGAGAAAATAAA 5 0.10532968190436064 No Hit TTCCGCGGGACTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA 5 0.10532968190436064 No Hit TTCCGCGGGGTGACAATTTATCACAACATCTAAAAACTCACAAAAATCACGGTGATTTTAGGGA 5 0.10532968190436064 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGTCTTCT 5 0.10532968190436064 Illumina Single End Adapter 2 (96% over 29bp) TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC 5 0.10532968190436064 No Hit TTCCGCGGGAGCTGGTTCAACGCAGAGTCGACTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGAGGGTATTTCAAGAATATAAATACATAGAAATGGTGCATCTTAACATTTGTTTGTA 5 0.10532968190436064 No Hit TTCCGCGGGAAGCAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT 5 0.10532968190436064 No Hit TTCCGCGGGACATGTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCAAATAACAAAATACATGAGGCTGCTTGGCTCAAACACATGAGCAGGCATTCCCGCAAA 5 0.10532968190436064 No Hit TTCCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGC 5 0.10532968190436064 No Hit TTCCGCTAGGGGGGGAAAATATTTATATGCAGGTTTAGCTGGTCCATTCTCCACGCTGTAAATA 5 0.10532968190436064 No Hit TTCCGCGGGAAGCTCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.10532968190436064 No Hit TTCCGCGGGGAGATTTAGAGCGGTTAAAACTGGAGATCGAATCTGGATTGAGAATCAGCACTGG 5 0.10532968190436064 No Hit TTCCGCGGTTCTGGAAATGTCTGTCAGTTTGCAGGAAACTGGAGTGTGGGTTCGCAGCCCTCTC 5 0.10532968190436064 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTG 5 0.10532968190436064 No Hit TTCCGCGGGAAGCACTGCATTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.10532968190436064 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.02106593638087213 0.0 0.0 0.0 0.0 9 0.04213187276174426 0.0 0.0 0.0 0.0 10 0.37918685485569836 0.0 0.0 0.0 0.0 11 0.6530440278070361 0.0 0.0 0.0 0.0 12 0.6951759005687803 0.0 0.0 0.0 0.0 13 0.7373077733305246 0.0 0.0 0.0 0.0 14 0.8637033916157573 0.0 0.0 0.0 0.0 15 0.9479671371392459 0.0 0.0 0.0 0.0 16 0.9479671371392459 0.0 0.0 0.0 0.0 17 0.9479671371392459 0.0 0.0 0.0 0.0 18 0.9479671371392459 0.0 0.0 0.0 0.0 19 0.9900990099009901 0.0 0.0 0.0 0.0 20 1.0743627554244786 0.0 0.0 0.0 0.0 21 1.0954286918053509 0.0 0.0 0.0 0.0 22 1.0954286918053509 0.0 0.0 0.0 0.0 23 1.1796924373288393 0.0 0.0 0.0 0.0 24 1.2850221192331999 0.0 0.0 0.0 0.0 25 1.2850221192331999 0.0 0.0 0.0 0.0 26 1.2850221192331999 0.0 0.0 0.0 0.0 27 1.3060880556140722 0.0 0.0 0.0 0.0 28 1.4324836738993048 0.0 0.0 0.0 0.0 29 1.5378133558036655 0.0 0.0 0.0 0.0 30 1.9170002106593638 0.0 0.0 0.0 0.0 31 1.9170002106593638 0.0 0.0 0.0 0.0 32 1.9170002106593638 0.0 0.0 0.0 0.0 33 1.9170002106593638 0.0 0.0 0.0 0.0 34 1.9591320834211081 0.0 0.0 0.0 0.0 35 2.148725510848957 0.0 0.0 0.0 0.0 36 2.169791447229829 0.0 0.0 0.0 0.0 37 2.2119233199915738 0.0 0.0 0.0 0.0 38 2.232989256372446 0.0 0.0 0.0 0.0 39 2.254055192753318 0.0 0.0 0.0 0.0 40 2.254055192753318 0.0 0.0 0.0 0.0 41 2.317253001895934 0.0 0.0 0.0 0.0 42 2.317253001895934 0.0 0.0 0.0 0.0 43 2.317253001895934 0.0 0.0 0.0 0.0 44 2.3383189382768066 0.0 0.0 0.0 0.0 45 2.3383189382768066 0.0 0.0 0.0 0.0 46 2.3593848746576787 0.0 0.0 0.0 0.0 47 2.3804508110385507 0.0 0.0 0.0 0.0 48 2.3804508110385507 0.0 0.0 0.0 0.0 49 2.401516747419423 0.0 0.0 0.0 0.0 50 2.422582683800295 0.0 0.0 0.0 0.0 51 2.422582683800295 0.0 0.0 0.0 0.0 52 2.422582683800295 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCAAA 15 0.004238676 58.54838 21 CTCCCAA 15 0.004238676 58.54838 20 CGGGGTG 35 1.2334567E-8 57.925533 6 AAGCAGT 85 0.0 57.925533 10 GCGGGGT 50 0.0 57.92553 5 GCGGGGG 45 2.0008883E-11 57.92553 5 GCGGGCA 20 1.8221354E-4 57.92553 5 GCGGGAG 45 2.0008883E-11 57.92553 5 GCGGGAC 20 1.8221354E-4 57.92553 5 GGAGACA 15 0.004425113 57.92553 55 CGGAGAC 15 0.004425113 57.92553 54 CGGGGGG 15 0.004425113 57.92553 6 CGGGGGC 15 0.004425113 57.92553 6 CGGGGAT 15 0.004425113 57.92553 6 GAAGCAG 90 0.0 57.92553 9 ACTCCCA 15 0.004425113 57.92553 19 TCGGAGA 15 0.004425113 57.92553 53 GGGAGTG 20 1.8221354E-4 57.92553 7 CGGGAGT 20 1.8221354E-4 57.92553 6 CGGGAAA 25 7.463137E-6 57.92553 6 >>END_MODULE