FastQCFastQC Report
Fri 17 Jun 2016
SRR1526983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4180
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1844.401913875598086No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA661.5789473684210527No Hit
TTCCGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA431.028708133971292No Hit
TTCCGCGGGAAGCACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.8133971291866028No Hit
TTCCGCGGGAGGGTTTACTCTTAAATACAATTAGAAAAAAAAAGTTGGCAAAACCT180.43062200956937796No Hit
TTCCGCGGGAAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.4066985645933014No Hit
TTCCGCGGGCTGGTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.4066985645933014No Hit
TTCCGCGGGAAGCACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.3827751196172249No Hit
TTCCGCGGGTACACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.3827751196172249No Hit
TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.3349282296650718No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG130.31100478468899523Illumina Single End Adapter 2 (95% over 23bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACT130.31100478468899523No Hit
TTCCGCGGGGGCTGCCAGCTCGCGCTTACCCACAGCCAACCAAAGGAACAAAGACC110.2631578947368421No Hit
TTCCGCGGGAAGCACTGCACTCTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2631578947368421No Hit
TTCCGCGGGAAGTACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2631578947368421No Hit
TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2631578947368421No Hit
TTCCGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC100.23923444976076555No Hit
TTCCGCGGGAAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA100.23923444976076555Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.23923444976076555No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG100.23923444976076555Illumina Single End Adapter 2 (96% over 30bp)
TTCCGCGGGAAGCACTGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.23923444976076555No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT90.21531100478468898Illumina Single End Adapter 2 (100% over 29bp)
TTCCGCGGGAAGCATTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.21531100478468898No Hit
TTCCGCGGGAAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.21531100478468898No Hit
TTCCGCGGGAAGCACTGCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.21531100478468898No Hit
TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA90.21531100478468898No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC80.19138755980861244Illumina Single End Adapter 2 (96% over 28bp)
TTCCGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA80.19138755980861244No Hit
TTCCGCGGGAAGCATTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19138755980861244No Hit
TTCCGCGGGAGTGTTATCAACGCGTAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT80.19138755980861244No Hit
TTCCGCGGGAAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19138755980861244No Hit
TTCCGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.19138755980861244No Hit
TTCCGCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG80.19138755980861244No Hit
TTCCGCGGGGCCTTTAGTTCTTCCCATTTTACTTTTAGATCACTTTCTGAATCCTC80.19138755980861244No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG80.19138755980861244No Hit
TTCCGCGGGAAGCATTATAACGCAGGGTCTCTTTTTTTTTTTTTTTTTTTTTTTTT70.1674641148325359No Hit
TTCCGCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1674641148325359No Hit
TTCCGCGGGAATTGAGAAAAGTGGTCTGAAGCTTCTGAAATGTCATATTTTCAAGG70.1674641148325359No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTT70.1674641148325359Illumina Single End Adapter 2 (100% over 27bp)
TTCCGCGGGAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.1674641148325359No Hit
TTCCGCGGGAGATGGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1674641148325359No Hit
TTCCGCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA70.1674641148325359No Hit
TTCCGCGGGAGACAAGCTTGTCGTGGTGGACTTCTCTGCTACGTGGTGTGGACCTT70.1674641148325359No Hit
TTCCGCGGGAAGCACTGTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1674641148325359No Hit
TTCCGCGGGAAGGGAGCCAGTGGAGATTCCCAGGCCTTTTGGCTTCCAGTCAGGTT60.14354066985645933No Hit
TTCCGCGGGAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT60.14354066985645933No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14354066985645933No Hit
TTCCGCGGGGTTGTTCAAGGGCGCATGGTGGATGCCTTGGCATGCTGAGCCGATGA60.14354066985645933No Hit
TTCCGCGGGAGGAAACAATAACGTAATTATCCTGGCTACCTCTTGGCTCAGAGGTA60.14354066985645933No Hit
TTCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC60.14354066985645933No Hit
TTCCGCGGGGTTTTGTTTTTTCCTTCCAACAAAGACTCTTGAAAAATCAAGACAAT60.14354066985645933No Hit
TTCCGCGGGGCACCAACAAAGTATCGTAGAAAGTACTTTAAAGCTTGCATCGTATG60.14354066985645933No Hit
TTCCGCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA60.14354066985645933Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGAGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14354066985645933No Hit
TTCCGCGGGCTGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14354066985645933No Hit
TTCCGCGGGGGGAAAAAAATAAATAAATAAAACTCTGATTGCCAAAACCACGTGCC60.14354066985645933No Hit
TTCCGCGGGGGGTGTGTTGGTGGGCACCCTGTAATCTCTGCTACTTGGGAGTCTGA60.14354066985645933No Hit
TTCCGCGGGTTCAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGATGGTTCAACGCAGAGTCGACTTTCTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGAAGCAGTGGTAGCCTTTCTGCCCAGCTCGAGCCACAGAGAAGGCAAC50.11961722488038277No Hit
TTCCGCGGGAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11961722488038277No Hit
TTCCGCGGGAGCATATCAACGCAGTGTCGACTCTTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGAAGCCCTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11961722488038277No Hit
TTCCGCGGGACTCACCCCACAAAAGACACAAAGAGAATCGCACAGCACACAGCAGG50.11961722488038277No Hit
TTCCGCGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC50.11961722488038277No Hit
TTCCGCGGGAAGCACTGTACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11961722488038277No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50.11961722488038277Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGAAGCGTTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGATCTTTGGAGTTGTAGTTATCTGATGTGAACCTCTATTCTTCACCCA50.11961722488038277No Hit
TTCCGCGGGGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTT50.11961722488038277No Hit
TTCCGCGGGATGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGAGCTGGTTCAACGCAGAGTCGACTTCTTTCTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGAGCCCAGCTTGAGAATCGGGCGCCGTCGGCGTTCGAATTGTAGTCTG50.11961722488038277No Hit
TTCCGCGGGGTGGCGGTGCGGATGTCAAAGATGAATTGTAAGTCCTTCGAGTTGCT50.11961722488038277No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCGCTTCC50.11961722488038277No Hit
TTCCGCGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCGAAAAAAAAAAAA50.11961722488038277No Hit
TTCCGCGGGGCAGAGTGCAGTGCTTTCCGCGAGAAGCCTTTAGGGAACATTGCCAG50.11961722488038277No Hit
TTCCGCGGGAAGCTGGTATCAACGCAGAGTCTTTTTTTTTTTCTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCAAATAACAAAATACATGAGGCTGCTTGGCTCAAACACATGAGCAGGCATT50.11961722488038277No Hit
TTCCGCGGGGGATATGCCCGGAGCTCCCTGCTGGCATCATGGCCTACAGAAGAGTC50.11961722488038277No Hit
TTCCGCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGT50.11961722488038277Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGACTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit
TTCCGCGGGCTATCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11961722488038277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAC203.7303302E-449.9638565
TTGCACT150.00787737949.96385615
CATTGCA150.00787737949.96385613
TCCCAAA150.00787737949.96385621
CTCCCAA150.00787737949.96385620
CGGGGGG150.00787737949.9638566
AGTGGTA950.049.96385614
GAAGCAT308.199913E-749.9638569
GAAGCAG1100.049.9638569
ACTCCCA150.00787737949.96385619
TCTGCCA150.00787737949.96385621
ATTGCAC150.00787737949.96385614
CGGGAGT203.7303302E-449.9638566
TGCCAAA150.00787737949.96385623
CACTCCC150.00787737949.96385618
AGCATTG150.00787737949.96385611
CGCGGGT150.00787737949.9638564
CTGCCAA150.00787737949.96385622
GTGGTAT900.049.96385615
GGGAAGG203.7303302E-449.9638567