FastQCFastQC Report
Fri 17 Jun 2016
SRR1526982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372005
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10000.26881359121517184No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8890.23897528259028775No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.16209459550274863No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5440.14623459362105348No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3920.10537492775634735No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3850.10349323261784117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA150.004664703758.0627951
GTCACTA150.004664703758.0627954
CACGTAA150.004664703758.0627952
AACACGT150.004667198358.05494750
ACTACCG150.004674687558.03144542
CACGTTA351.6336344E-858.01578538
GTCGATC150.00468969357.98449335
GTAGCGA150.00469720957.96104432
CGATAAA150.00469720957.96104431
CCGTCGA258.7622975E-657.88302617
TTCGGTA150.004722327557.88302217
ACTAATG150.004747546357.80521419
ATTGTGG386300.054.6650922
TGTGGGG185100.054.593764
TTGTGGG375000.054.5612953
TATTGTG391900.054.411691
TGGGACA7050.054.400736
GTGGGAT37100.053.8025325
TGGGATT10600.053.7242166
GTGGGGG87850.053.5457425