FastQCFastQC Report
Fri 17 Jun 2016
SRR1526981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489824
Sequences flagged as poor quality0
Sequence length64
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC30610.6249183380152871No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC27640.5642843143659764No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC26760.5463186777291436No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT22050.45016169072973156No Hit
TATTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC18840.38462794799764816No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC14630.2986787090873457No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT14300.29194159534853337No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9810.20027601750832952No Hit
TATTGTGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC8850.18067714117723918No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.17679819690337753No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG8630.17618573201803098No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC8160.166590448814268No Hit
TATTGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG7330.1496455869863461No Hit
TATTGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT6770.1382129091265434No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6350.1296384007316914No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG5920.12085973737505716No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTC5780.11800156791010649No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT5250.10718135493565037No Hit
TATTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA5160.10534396027961064No Hit
TATTGTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC5120.10452734043248187No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT5120.10452734043248187No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGAAGGTCA4920.10044424119683805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA150.004644783758.12837651
CGATTAG201.98839E-458.12241448
ACCGTAC150.004657997358.0866841
TACCGTA150.00467502758.0331735
TCGACAC202.0045669E-458.02722534
CTATCGT150.004678817558.0212932
ACGTTAC150.004724484457.87912817
GTGGGAT49100.054.8899965
ATTGTGG514950.054.327142
TTGTGGG501400.054.2710843
TATTGTG519800.054.254751
TGTGGGG246450.054.126334
GTGGGGC48150.053.628245
TGTGGGC53950.053.174964
GTGGGGT42550.052.7943085
GTGGGGG115950.052.7537965
GTGGGAC30500.052.202085
GTGGGGA45950.052.100875
TGGGATG15800.052.0338826
TGTGGGA178700.052.000254
Produced by FastQC (version 0.11.5)