FastQCFastQC Report
Fri 17 Jun 2016
SRR1526980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487414
Sequences flagged as poor quality0
Sequence length64
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC27570.5656382459264608No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC25950.5324016134128277No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC24380.500190802890356No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT19800.40622550849996103No Hit
TATTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC17830.36580812204819724No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT13920.28558884233936654No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC12880.26425174492320697No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9090.18649443799316393No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG8420.17274842331159956No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.1688502997451858No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7850.16105405261235828No Hit
TATTGTGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC7720.15838691543533834No Hit
TATTGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6870.14094794158559254No Hit
TATTGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT6040.12391929653231135No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5810.11920051537296836No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTC5310.10894229546135317No Hit
TATTGTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC5280.10832680226665627No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG4900.10053055513382872No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT4880.10012022633736413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT150.004537288658.4730742
TCGACAG201.9954826E-458.08046750
TATCGCT150.004659947558.08046751
CGATTAG258.5964275E-658.0744748
CGCAACT201.9965018E-458.07446747
ATCGGCC150.004663760358.06847455
TACCGTT150.004663760358.06847458
ACATCGG150.004667575558.0564853
CGGTAGG650.057.86528431
CGTGACG258.927926E-657.70494519
CCGTCGA303.9002225E-757.68718717
TCGGTAG150.004787002757.68718718
GTGGGAT49050.054.806245
TGTGGGG242700.054.3220754
ATTGTGG510750.054.26892
TTGTGGG494500.054.1700133
TATTGTG516900.054.1660651
GTGGGGG109250.053.4990125
TGGGATC14600.053.0600936
GTGGGGC53300.052.9308245
Produced by FastQC (version 0.11.5)