FastQCFastQC Report
Fri 17 Jun 2016
SRR1526979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433198
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC47521.0969579730284997No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC30180.6966791167087568No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC24500.5655612445117475No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT18540.4279798152346041No Hit
TATTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC15350.3543414327859316No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC11360.2622357443940185No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9170.2116814943743969No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8120.18744315532389347No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC7470.17243846924501036No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC7440.17174594527213882No Hit
TATTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6860.1583571484632893No Hit
TATTGTGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC6830.1576646244904178No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6180.14265993841153468No Hit
TATTGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6020.13896647722288655No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5970.137812270601434No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC5850.13504217470994787No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5550.1281169349812326No Hit
TATTGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5050.11657486876670714No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA4930.11380477287522103No Hit
TATTGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4770.11011131168657289No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA4700.10849542241653932No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT4650.10734121579508678No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC4640.10711037447079627No Hit
TATTGTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT4510.10410943725501964No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC4380.10110850003924303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA150.008330394550.1467337
TTCGATC150.00834188650.12924235
TACTAGC150.00837258850.08267633
CTATCGT150.008384122550.06523532
ATCGAAT252.0835143E-550.0071929
ATTCGCT204.2356824E-449.86842323
GTCGCGA150.00857029349.78783417
GATCGTA150.00857029349.78783410
CTGCGTA252.138412E-549.78783417
TCGTCAC150.00857029349.78783412
GTGGGAT44800.047.5761455
TGTGGGG218950.047.126844
TGGGATC12800.047.0759286
TTGTGGG438350.047.0557373
ATTGTGG451650.047.0372962
TATTGTG456600.047.001851
TGGGATT14500.046.880066
GTGGGGC47700.046.7194945
GTGGGGG98350.046.40685
GTGGGGT37250.045.855495
Produced by FastQC (version 0.11.5)