FastQCFastQC Report
Fri 17 Jun 2016
SRR1526978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96141
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.32348321735783897No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2220.23091084968951853No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.22362987695156072No Hit
GCAGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.11753570276989006No Hit
GCAGGAGGGCCTGCTGGCATATGTGTCTGATCTGGTGTACTCCACTCGTCTGGTGTTCGTCAAG1120.11649556380732466No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1090.11337514691962845No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.11129486899449767No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1060.11025473003193227No Hit
GCAGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10713431314423606No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1000.10401389625653988No Hit
GCAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.10297375729397447No Hit
GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.10297375729397447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTA150.00449991658.5587856
TACCACT150.00449991658.5587856
GCCATAT150.00449991658.5587858
TCGTCCA150.00449991658.5587857
TCGTCAA351.5017577E-858.5587857
CGTCAAG351.5017577E-858.5587858
ATGGTAG201.9083197E-458.55877756
CTATTAT201.9083197E-458.55877757
GGTAGAC201.9083197E-458.55877758
AGGGTGC406.9303496E-1058.1297766
TTTACTA150.004652180758.06953
CGAGTCC150.004652180758.06948
TATGAAA150.004652180758.06950
CTACACG150.004652180758.06946
AAAATTC150.004652180758.06947
CTGATAT201.9893002E-458.06954
GGAGATA201.9944509E-458.0386628
CGCGCTA150.00466182358.03866241
ATAGCCA150.00466182358.03866242
ACTTCAT201.9944509E-458.03866243
Produced by FastQC (version 0.11.5)