FastQCFastQC Report
Fri 17 Jun 2016
SRR1526977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132233
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC7210.5452496729258204No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4600.34787080380842905No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3670.2775404021688988No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3600.272246716023988No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.2707342342682992No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.2609031028563218No Hit
GCAGGAGGGGAGTCGGAGGCTTCGACGGCAGTACCGGCCTGGCATCCGTAGAAGCCTACAGCTA2550.1928414238503248No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2470.18679149682756951No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT2430.18376653331619186No Hit
GCAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC2280.17242292014852573No Hit
GCAGGAGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCCAGAGGTCC2230.16864171575930365No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2060.15578562083594866No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC2040.15427313908025986No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.14897945293534898No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT1880.14217328503474927No Hit
GCAGGAGGGCCTGCTGGCATATGTGTCTGATCTGGTGTACTCCACTCGTCTGGTGTTCGTCAAG1860.14066080327906044No Hit
GCAGGAGGGAACGTACACTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCTTACC1780.13461087625630516No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA1710.12931719011139428No Hit
GCAGGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGAAGGTCC1710.12931719011139428No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1700.12856094923354985No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1690.12780470835570545No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTCCGAAGGTC1650.12477974484432781No Hit
GCAGGAGGGGGGCCAGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT1610.12175478133295016No Hit
GCAGGAGGGGTTCCATGCTCCATCTGTGGGTGTCTACAACAGCTTTCTTCTCCCTGATATTTAT1570.11872981782157252No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1530.11570485431019488No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGTCTGCCCTTCCAGAGGTCCT1430.10814244553175077No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCAGAGGTCCC1380.10436124114252873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACC258.502522E-658.1425954
CGACTAA258.502522E-658.1425952
ATCTACC201.9796292E-458.14258647
CGAGTCC150.004632772458.14258648
TCACATC150.004632772458.14258646
TTGTCGC150.004632772458.14258655
ACCGAAG201.9796292E-458.14258655
CCTACAC150.004632772458.14258646
GGTCATA150.004632772458.14258645
ACTACTA150.004632772458.14258650
AATCTGT150.004632772458.14258656
TCGTCAA201.9796292E-458.14258657
GTACTCG150.004632772458.14258646
CTACACC150.004632772458.14258647
TACTACC150.004632772458.14258652
TGCTACT406.9667294E-1058.14258647
TCATAGC201.9796292E-458.14258647
CGACTAC150.004632772458.14258648
CGTCAAG201.9796292E-458.14258658
ATACCTA150.004632772458.14258654
Produced by FastQC (version 0.11.5)