FastQCFastQC Report
Fri 17 Jun 2016
SRR1526976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131181
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6510.49626089144007135No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4250.3239798446421357No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3550.2706184584657839No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.2614707922641236No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.25003620951204825No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3100.2363147102095578No Hit
GCAGGAGGGGAGTCGGAGGCTTCGACGGCAGTACCGGCCTGGCATCCGTAGAAGCCTACAGCTA2280.17380565783154572No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2240.17075643576432564No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.16923182473071557No Hit
GCAGGAGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCCAGAGGTCC2160.16465799162988543No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT2140.16313338059627538No Hit
GCAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC2010.15322340887781005No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC1820.13873960405851457No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGTCTGCCCTTCCAGAGGTCCT1780.13569038199129446No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1780.13569038199129446No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1740.13264115992407435No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT1710.13035424337365928No Hit
GCAGGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGAAGGTCC1690.12882963234004927No Hit
GCAGGAGGGAACGTACACTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCTTACC1660.1265427157896342No Hit
GCAGGAGGGCCTGCTGGCATATGTGTCTGATCTGGTGTACTCCACTCGTCTGGTGTTCGTCAAG1530.11663274407116885No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1520.11587043855436382No Hit
GCAGGAGGGGGGCCAGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT1510.11510813303755879No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTCCGAAGGTC1500.11434582752075377No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA1400.10672277235270351No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCAGAGGTCCC1370.10443585580228844No Hit
GCAGGAGGGGTTCCATGCTCCATCTGTGGGTGTCTACAACAGCTTTCTTCTCCCTGATATTTAT1320.10062432821826331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA150.004517058458.51469826
TGTCGGC150.004517058458.51469826
CATTCTG150.00453091358.4694823
ATCTTAA150.00453091358.4694823
CAGGCCG258.2427705E-658.44689622
GTAACAC258.2427705E-658.44689644
GGTCATA351.5304977E-858.4468945
ATCCGTA351.5304977E-858.4468944
CGGTCAT303.5425364E-758.4468944
TCGACGG452.910383E-1158.4468922
ACCTTAT150.00453785258.4468945
CTAATCC150.00453785258.4468945
CTAATAC150.00453785258.4468922
TGAGTCT201.929089E-458.44688822
AGTCTAA150.00454479958.42432841
GTGTAAC303.5520134E-758.42432842
GGACGAC150.00454479958.42432840
GTATATG150.00454479958.42432839
GACGACA150.00454479958.42432841
AGCTAAT150.00454479958.42432843
Produced by FastQC (version 0.11.5)