SRR1526975_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	115600
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1147	0.9922145328719724	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	783	0.6773356401384083	No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	334	0.2889273356401384	No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	318	0.2750865051903114	No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	273	0.23615916955017302	No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	265	0.2292387543252595	No Hit
GCAGGAGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCC	210	0.18166089965397925	No Hit
GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	204	0.17647058823529413	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC	198	0.171280276816609	No Hit
GCAGGAGGGGAGTCGGAGGCTTCGACGGCAGTACCGGCCTGGCATCCGTAGAAGCC	187	0.16176470588235295	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	166	0.14359861591695502	No Hit
GCAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	165	0.14273356401384082	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA	164	0.14186851211072665	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	159	0.1375432525951557	No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCT	156	0.13494809688581313	No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	151	0.13062283737024222	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTC	149	0.12889273356401384	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCA	145	0.12543252595155707	No Hit
GCAGGAGGGGAGCCCGCTCCCCGAGCAGCCTTAGCCTTCCCTAGCTAATCCAACTG	142	0.12283737024221453	No Hit
GCAGGAGGGGGGCCAGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA	142	0.12283737024221453	No Hit
GCAGGAGGGCCTGCTGGCATATGTGTCTGATCTGGTGTACTCCACTCGTCTGGTGT	142	0.12283737024221453	No Hit
GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	139	0.12024221453287197	No Hit
GCAGGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	135	0.11678200692041521	No Hit
GCAGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133	0.11505190311418687	No Hit
GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.11418685121107268	No Hit
GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	132	0.11418685121107268	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	129	0.11159169550173012	No Hit
GCAGGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	128	0.11072664359861592	No Hit
GCAGGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT	125	0.10813148788927336	No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGTCTGCCCTTCCA	123	0.10640138408304499	No Hit
GCAGGAGGGGTTCCATGCTCCATCTGTGGGTGTCTACAACAGCTTTCTTCTCCCTG	123	0.10640138408304499	No Hit
GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT	116	0.10034602076124569	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATATT	15	0.00799178	50.651848	50
TACACCT	15	0.00799178	50.651848	48
ATGATCC	15	0.008230917	50.275665	47
CCTACAC	15	0.008230917	50.275665	46
TATGATC	15	0.008230917	50.275665	46
ATCCGTA	35	5.0116796E-8	50.275665	44
GCCTACA	15	0.008230917	50.275665	45
AGCCTAC	15	0.008230917	50.275665	44
ATAAACT	15	0.008230917	50.275665	46
GCTCCCA	15	0.008230917	50.275665	44
ATCCTCT	25	2.0143894E-5	50.25371	42
TAGCCTA	15	0.008245148	50.25371	43
CCACTCG	20	4.0652114E-4	50.25371	42
CCTGTAT	15	0.008302254	50.166084	41
GGCATCC	35	5.0986273E-8	50.166084	41
TACCTGT	15	0.0083165765	50.144226	39
GTACTCC	20	4.11808E-4	50.122387	37
GTTTGGT	15	0.008345276	50.100567	36
GCAAAAG	15	0.008345276	50.100567	36
GTAGGAT	15	0.008345276	50.100567	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content