##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526975_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115600 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.347292387543256 39.0 38.0 40.0 36.0 40.0 2 37.965207612456744 39.0 38.0 40.0 35.0 40.0 3 37.837335640138406 39.0 38.0 40.0 35.0 40.0 4 38.15647923875432 39.0 38.0 40.0 35.0 40.0 5 38.024134948096886 39.0 38.0 40.0 35.0 40.0 6 36.883901384083046 39.0 36.0 39.0 32.0 40.0 7 38.55051903114187 39.0 38.0 40.0 37.0 40.0 8 38.53689446366782 39.0 38.0 40.0 37.0 40.0 9 37.96044982698962 39.0 38.0 40.0 35.0 40.0 10 37.15006055363322 39.0 35.0 40.0 32.0 40.0 11 36.97969723183391 39.0 35.0 40.0 32.0 40.0 12 37.18498269896194 39.0 36.0 40.0 32.0 40.0 13 37.02798442906575 39.0 36.0 40.0 32.0 40.0 14 37.33569204152249 39.0 37.0 40.0 33.0 40.0 15 37.3211937716263 39.0 37.0 40.0 33.0 40.0 16 37.28036332179931 39.0 37.0 40.0 33.0 40.0 17 37.046271626297575 38.0 36.0 39.0 33.0 40.0 18 36.926764705882356 38.0 36.0 39.0 33.0 40.0 19 36.787058823529414 38.0 36.0 39.0 32.0 40.0 20 36.80833044982699 38.0 36.0 39.0 32.0 40.0 21 36.57242214532872 38.0 36.0 39.0 32.0 40.0 22 36.37150519031142 38.0 35.0 39.0 31.0 40.0 23 36.2732785467128 38.0 35.0 39.0 31.0 40.0 24 36.18377162629758 38.0 35.0 39.0 31.0 40.0 25 36.04794117647059 38.0 35.0 39.0 30.0 40.0 26 35.579195501730105 38.0 35.0 39.0 29.0 40.0 27 35.315960207612456 38.0 35.0 39.0 29.0 40.0 28 35.09838235294118 38.0 34.0 39.0 28.0 40.0 29 34.96174740484429 38.0 34.0 39.0 28.0 40.0 30 34.67176470588235 38.0 33.0 39.0 27.0 40.0 31 33.94871107266436 38.0 33.0 39.0 25.0 40.0 32 33.851029411764706 38.0 33.0 39.0 24.0 40.0 33 33.66384948096886 37.0 33.0 39.0 23.0 40.0 34 33.77464532871972 37.0 33.0 39.0 24.0 40.0 35 33.46660034602076 37.0 32.0 39.0 23.0 40.0 36 33.532681660899655 38.0 33.0 39.0 22.0 40.0 37 33.41217993079585 37.0 33.0 39.0 22.0 40.0 38 33.088901384083044 36.0 32.0 39.0 20.0 40.0 39 32.94795847750865 36.0 32.0 39.0 18.0 40.0 40 32.70792387543253 36.0 32.0 39.0 17.0 40.0 41 32.38652249134948 36.0 32.0 39.0 9.0 40.0 42 32.19961937716263 36.0 31.0 39.0 4.0 40.0 43 32.01735294117647 36.0 31.0 39.0 4.0 40.0 44 31.772897923875433 36.0 31.0 39.0 4.0 40.0 45 31.34756920415225 36.0 30.0 39.0 4.0 40.0 46 30.61120242214533 35.0 30.0 39.0 4.0 40.0 47 30.324152249134947 35.0 29.0 39.0 4.0 40.0 48 29.966167820069206 35.0 29.0 39.0 4.0 40.0 49 29.730544982698962 35.0 28.0 39.0 4.0 40.0 50 29.28794982698962 35.0 27.0 39.0 4.0 40.0 51 28.401522491349482 35.0 25.0 38.0 4.0 39.0 52 28.114282006920416 34.0 24.0 38.0 4.0 39.0 53 27.773460207612455 34.0 23.0 38.0 4.0 39.0 54 27.28113321799308 34.0 17.0 38.0 4.0 39.0 55 26.917854671280278 34.0 4.0 38.0 4.0 39.0 56 15.836946366782007 19.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.4121097829729692 1 2 -0.9031916921456116 1 3 -0.5840626107447946 1 4 -0.764069753525348 1 5 -1.4583787184675145 1 6 -0.605690430634418 1 7 -0.4583002207950173 1 8 -0.5115727458749006 1 9 -0.9042529035421296 1 10 -0.8370492410060066 1 11 -0.6777700770636059 1 12 -0.88825228323131 1 13 -0.9299916817034628 1 14 -0.8912172712337991 1 15 -0.7695087023598219 1 16 -0.5498357784273225 1 17 -0.4213477718730161 1 18 -0.6512912994282942 1 19 -0.42774346600611324 1 20 -1.2031309543377375 1 21 -0.7345442998498086 1 22 -1.439001077751385 1 23 -0.6645518884847945 1 24 -0.2590660858628766 1 25 -1.228805184213968 1 26 0.7406640004783327 1 27 0.04766151751018555 1 28 0.49850553068628756 1 29 0.5799630371678077 1 30 0.48437019232400047 1 31 0.4373969204529615 1 32 1.2490731466482075 1 33 0.7557936368369056 1 34 0.20391635005824327 1 35 0.796927351798189 1 36 0.6024886463431827 1 37 1.8184510383827899 1 38 0.9403828369778466 1 39 0.9860255275827257 1 40 0.8132749848610743 1 41 1.3876471764810532 1 42 1.273616307240843 1 43 1.58895592429824 1 44 1.8378198900452105 1 45 1.733666423232446 1 46 1.5807704545850463 1 47 1.8137270838616786 1 48 1.3318440339064566 1 49 2.2581215810583366 1 50 1.7397491256986584 1 51 2.308242687940279 1 52 2.2662056271777473 1 53 2.02117627603047 1 54 1.6513399711040506 1 55 2.040824738712889 1 56 1.7886137644299716 2 1 -0.37409808706653536 2 2 -0.22385446017680266 2 3 -0.30774682127110964 2 4 -0.16221790167349326 2 5 -0.19083485881839124 2 6 -0.3600763955466988 2 7 0.010510695384319035 2 8 -0.014009393048389995 2 9 -0.5402178158228352 2 10 -0.77613306166878 2 11 -0.7840078938277415 2 12 -1.0632035502878452 2 13 -0.4382762820933266 2 14 -0.6188001172376971 2 15 -0.582861528870545 2 16 -0.2316096575696207 2 17 -0.5266109297677488 2 18 -0.525560305276251 2 19 -0.48329902156166327 2 20 -0.5262303695424109 2 21 -0.29838445579522244 2 22 -0.7991375299931036 2 23 -0.6460333699662755 2 24 -0.8282668655899741 2 25 -0.7229572309975936 2 26 -0.5941313211590966 2 27 -0.3651065136788958 2 28 -0.6121182509901288 2 29 0.3192417896044546 2 30 -0.5633880922763908 2 31 -0.13521516531702105 2 32 0.5848431271550325 2 33 0.028210790833007593 2 34 -0.5670388156337651 2 35 0.20384742977090298 2 36 0.50014946505663 2 37 0.717573845400338 2 38 1.1859968720655658 2 39 0.5084426815788277 2 40 1.0116180647831001 2 41 1.9773157924654612 2 42 1.4485675742973783 2 43 1.9042580685477475 2 44 2.369496303300572 2 45 2.2083252926281567 2 46 1.70114082495542 2 47 2.0008615867856534 2 48 0.79675631460821 2 49 1.4506167077639915 2 50 2.069671152989109 2 51 1.645474656751194 2 52 1.8787787265929516 2 53 1.6449579524437254 2 54 1.3555310042424509 2 55 1.9779592416368672 2 56 0.7471907624806526 3 1 -0.02322089408407635 3 2 -0.19398248293640563 3 3 -0.30253107921326006 3 4 -0.15320889266448745 3 5 -0.12279313288193094 3 6 -0.038122863066853085 3 7 -0.06440632690112835 3 8 -0.03534651964867663 3 9 0.057458808169577935 3 10 -0.06252471648148372 3 11 -0.6512435505370817 3 12 -0.08620023117926223 3 13 -0.006318008029786881 3 14 -0.7513273813439056 3 15 -0.2817709646220905 3 16 -0.17471065330219915 3 17 -0.24116759169283597 3 18 -0.6317717799087745 3 19 -0.3211368593995019 3 20 0.10392610271932057 3 21 0.35879904349354064 3 22 0.7769648882145788 3 23 0.13775041381751407 3 24 0.7414344146376237 3 25 0.4138374584286808 3 26 1.037210547024877 3 27 0.6825464023950687 3 28 1.193950976131731 3 29 1.5541872613920305 3 30 1.2706564786102845 3 31 1.0262357592918008 3 32 0.4935676411427039 3 33 1.4046925357358049 3 34 0.6413537874956816 3 35 1.7040845089553471 3 36 0.7571433009978392 3 37 0.6900726600044109 3 38 0.917860314455325 3 39 0.875915417472612 3 40 0.8385502601363513 3 41 1.2445040333379112 3 42 1.300393084017621 3 43 0.7733903587326694 3 44 0.1513834536087728 3 45 1.0765092660752877 3 46 0.2732128970274914 3 47 0.22063399076858303 3 48 0.6967088987713197 3 49 0.6497632226999812 3 50 0.39115052710005926 3 51 1.012947392644982 3 52 1.0807701917423103 3 53 1.1117668666210605 3 54 1.1616002313643108 3 55 1.0445784924666448 3 56 0.22680195261816039 4 1 -0.14746331832650128 4 2 -0.059420648374569396 4 3 -0.31588079256297874 4 4 -0.6258542653098544 4 5 -0.25989386998266895 4 6 -0.4753874003313925 4 7 0.07637991388511267 4 8 0.12394913964698162 4 9 -0.27849532778455455 4 10 -0.6796418335986019 4 11 -0.23871283800637144 4 12 0.46040091542188577 4 13 0.3762372745254936 4 14 0.31804198802546324 4 15 0.20103001817889776 4 16 0.4267635481720049 4 17 0.21198556146031677 4 18 0.443658195521202 4 19 0.3924585541959118 4 20 0.5488892476824674 4 21 0.10400788870238387 4 22 -0.09371488246519277 4 23 0.2691181451852387 4 24 0.5139978872011 4 25 0.5277604723516944 4 26 0.5340983439126745 4 27 1.0565840764327419 4 28 1.1239264061071594 4 29 1.1686835758883447 4 30 0.8345385424923535 4 31 1.7911006241566625 4 32 1.2347633823384427 4 33 0.5892953203385929 4 34 0.392131838273734 4 35 1.3344694393402818 4 36 1.170165414019948 4 37 0.6017843717161284 4 38 0.9373525339475464 4 39 1.1846787262359229 4 40 1.0545207761068696 4 41 2.315593304427182 4 42 1.8431449267694617 4 43 1.6099996953420117 4 44 1.9790656812910044 4 45 1.0915788811449012 4 46 2.4375044613190546 4 47 2.7723129424475346 4 48 2.0045713066337285 4 49 2.4821956551324114 4 50 2.4173585533080875 4 51 3.027771307468896 4 52 2.8838823948545134 4 53 2.9487857036398992 4 54 2.5582423280064077 4 55 3.1645621124502625 4 56 2.0106676364838414 5 1 -0.2747360455992336 5 2 -0.5230570120109306 5 3 -0.3976989743811572 5 4 -0.20767244712804 5 5 -0.23262114270993806 5 6 -0.9481146730586616 5 7 -0.1690746315694298 5 8 -0.439687223989381 5 9 -0.6966771459663761 5 10 -0.82509637905315 5 11 -1.2023492016427326 5 12 -0.8759627209417502 5 13 -0.4146718163835956 5 14 -0.3819580119745396 5 15 -0.5171518000029209 5 16 -0.42778190637345404 5 17 0.08471283418759157 5 18 -1.6654327135697073 5 19 -0.25299599125863637 5 20 -0.33292893413571534 5 21 0.07673516142965298 5 22 0.13355784480753385 5 23 0.050936327003427095 5 24 0.25945243265564244 5 25 -0.5995122549210379 5 26 0.5795528893672213 5 27 0.8384022582509232 5 28 0.25119913337988464 5 29 -0.16768006047529127 5 30 0.052720360674172184 5 31 0.47291880597484237 5 32 -0.3743275267524666 5 33 -0.23797740693413516 5 34 0.0830409291828218 5 35 0.11628762115846314 5 36 0.25198359583813357 5 37 -1.2073065373747838 5 38 0.037352533947547784 5 39 -0.1153212737640743 5 40 0.15452077610686388 5 41 -0.8389521501182706 5 42 -0.47503689141235483 5 43 -0.6354548501125379 5 44 -0.5663888641635424 5 45 -0.2720574824914621 5 46 0.29204991586451 5 47 0.20867657881117196 5 48 0.7772985793610019 5 49 0.9276502005869567 5 50 0.1446312805808141 5 51 -0.1358650561674679 5 52 0.22933694030906082 5 53 -1.2330324781782842 5 54 -0.4417576719935923 5 55 0.019107566995717917 5 56 0.35612218193838885 6 1 -0.026278998059616754 6 2 -0.22514208290334636 6 3 0.05584564613594267 6 4 -0.19891514937991417 6 5 -0.014189116021036341 6 6 -0.20507880983931415 6 7 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48 1.5015688045486577 6 49 1.1647644624718616 6 50 0.8752401212813972 6 51 1.5658030005464632 6 52 2.0044828952715292 6 53 1.0947406661086205 6 54 1.8337218943116618 6 55 1.1032610282133994 6 56 0.6804758099617416 7 1 0.22835143295994698 7 2 0.3061025077146269 7 3 0.38291852133068005 7 4 0.1679707775717887 7 5 0.14696719348216902 7 6 0.6647498209379066 7 7 0.29816379038597063 7 8 0.3100554861306861 7 9 0.36147036689811785 7 10 0.35809401545800057 7 11 0.6590573163675586 7 12 0.27823968043045966 7 13 0.0541103448513951 7 14 -0.23101461574812276 7 15 0.18559262538300914 7 16 0.049919637365825054 7 17 0.49689122183767864 7 18 0.06149696052977305 7 19 0.31544311680311665 7 20 0.20514996809413333 7 21 0.39526003278471933 7 22 0.7592868328006759 7 23 -0.18062456493482415 7 24 0.3537920552971485 7 25 0.8728719646329921 7 26 1.3323830780464618 7 27 1.2648173525905477 7 28 1.9532574524193365 7 29 2.280347383778569 7 30 2.158723791205901 7 31 2.0043081713264783 7 32 1.6978851662149452 7 33 1.7304617011276164 7 34 2.48973046606104 7 35 1.890044053405461 7 36 2.5228240761125704 7 37 2.505557956621786 7 38 2.1416406986130667 7 39 2.289309944074688 7 40 2.298774635455068 7 41 2.669967232386014 7 42 2.6825960416922783 7 43 2.3124010675547026 7 44 2.634640295356185 7 45 2.5886629291723473 7 46 2.946423843823343 7 47 3.356704023065035 7 48 1.822410071642306 7 49 2.7991767872078874 7 50 2.6120412291228376 7 51 2.8257129884294443 7 52 2.728993887135818 7 53 2.992524983228236 7 54 3.153611110167642 7 55 3.299210482947693 7 56 2.265727670789161 8 1 -0.5074976236438289 8 2 -0.9014446620967007 8 3 -1.1642512899900765 8 4 -0.46410469412632693 8 5 -0.6737875234989659 8 6 -1.6654388583073754 8 7 -0.36221356810459326 8 8 -0.18994451386931388 8 9 -0.4970201991396195 8 10 0.027905336212718623 8 11 -0.4541502308022558 8 12 0.18390005778895357 8 13 -0.1723047494882266 8 14 0.0708721767047038 8 15 -0.4370488840509523 8 16 0.1536932222714853 8 17 0.1195327312716401 8 18 0.08979884732222843 8 19 -0.36380216621575556 8 20 -0.153340597943604 8 21 0.2914864478790591 8 22 0.22155098374407345 8 23 0.4231490199708361 8 24 0.7688863949197966 8 25 0.7974002665197872 8 26 1.5021943988011799 8 27 1.0667041450433814 8 28 1.3589178297778304 8 29 1.2803473837785688 8 30 0.5455162440360937 8 31 0.5797798694396832 8 32 1.348828562441355 8 33 -0.2789722611365377 8 34 1.1218059377591558 8 35 1.6164591477450827 8 36 1.7869750195088017 8 37 1.024425881150087 8 38 1.6039048495564643 8 39 1.5723288119992134 8 40 1.6006614279078946 8 41 2.1039294965369564 8 42 2.229765853013035 8 43 1.8312689920830039 8 44 1.81388557837505 8 45 1.220738400870463 8 46 0.9558578060874972 8 47 1.6774587400461627 8 48 1.6903345999441939 8 49 1.4878560324909031 8 50 1.6214751913869883 8 51 2.1653356299388804 8 52 1.710125962607517 8 53 2.303845737945217 8 54 2.7196488460166997 8 55 2.7426067093627857 8 56 2.294029557581613 9 1 0.1532496941512136 9 2 0.18068630349709025 9 3 0.03848640886305077 9 4 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-1.4293366457121088 120 43 -1.1004835151412031 120 44 -1.5423102400849196 120 45 -1.4910583014922807 120 46 -1.88894926513467 120 47 -2.4550416849070906 120 48 -1.8168046147421926 120 49 -1.8727592998225404 120 50 -2.743984608035074 120 51 -1.8699354902379 120 52 -1.6309415199694008 120 53 -1.5729178180636225 120 54 -1.4600213902573103 120 55 -1.5410070931189424 120 56 -0.034459308643102204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 37.0 5 17.0 6 16.0 7 40.0 8 67.0 9 72.0 10 68.0 11 125.0 12 163.0 13 243.0 14 358.0 15 399.0 16 490.0 17 627.0 18 899.0 19 935.0 20 973.0 21 1179.0 22 1109.0 23 1200.0 24 1611.0 25 1647.0 26 1722.0 27 2111.0 28 2332.0 29 2645.0 30 3125.0 31 3702.0 32 4846.0 33 5896.0 34 7640.0 35 10104.0 36 13536.0 37 18297.0 38 18045.0 39 9324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.33737024221453 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31.436258099118504 23.57373333679357 22.24673223816815 22.743276325919776 21 29.15051903114187 24.389273356401382 23.8780276816609 22.58217993079585 22 27.42820069204152 21.922145328719722 28.507785467128027 22.141868512110726 23 31.639273356401382 19.80795847750865 26.878892733564015 21.673875432525953 24 33.75523410734678 20.203654358583936 25.06921133681697 20.97190019725231 25 29.925674681802843 19.37822847897 23.344552793472523 27.351544045754633 26 25.063402896192365 18.246185006621598 30.15900494239641 26.531407154789623 27 22.911599484121144 16.7487514173685 29.77988591806528 30.559763180445078 28 22.67826990518249 21.84959085595532 28.401091050785816 27.071048188076375 29 27.480135520375715 21.254343324061796 27.810271474000707 23.455249681561778 30 26.78230702515178 18.777970511708585 31.383347788378146 23.056374674761493 31 28.440645609163482 16.868274904547032 32.96858729607775 21.72249219021173 32 31.507978515709738 16.132307131639003 32.46158251407846 19.898131838572805 33 30.627158950858373 16.82896769601972 35.01102258327692 17.53285076984499 34 24.76211973885262 17.536289762467007 40.73829698569246 16.963293512987914 35 21.393272479351044 22.85237842955037 38.93641598415828 16.81793310694031 36 21.45122194903695 27.417182870993805 34.73671407348201 16.394881106487233 37 25.913857970787824 24.069198620198282 33.36113790197152 16.655805507042377 38 25.53049072021559 24.4090928847742 33.26118137958013 16.799235015430085 39 27.445541110483063 22.507065524588025 32.72924909778686 17.31814426714205 40 24.687780127585874 19.30149778508829 32.51873319234485 23.491988894980985 41 21.32580142572396 22.211874069754284 33.82482221970772 22.637502284814037 42 19.928968122704084 20.503490659656332 33.9194623861836 25.64807883145598 43 19.9512301328108 18.25908991944263 36.36403222294796 25.425647724798605 44 21.933697327516395 16.96230309046736 35.95008577374888 25.15391380826737 45 23.51511455701716 19.440717832563813 35.14069169788309 21.903475912535935 46 22.40632435871735 20.177632897821862 34.47803993689587 22.93800280656492 47 20.144211067807102 18.94361469313733 36.92204406546171 23.990130173593855 48 22.621363457931697 18.51615998604266 37.72582544598072 21.136651110044923 49 24.65076126196829 17.70697082265125 36.03219449240483 21.61007342297564 50 21.228806534088427 18.196493861202978 36.64080838401731 23.93389122069128 51 19.32028333158868 18.856205729439267 37.55626504762902 24.267245891343034 52 19.96160725971816 18.618733912132978 37.81772173988918 23.601937088259675 53 19.734568870739754 18.523462994956635 39.635795682599515 22.106172451704097 54 20.117412358178 18.798280998708112 38.03685834058372 23.047448302530167 55 20.021650690552267 19.116337541249802 34.74935833638887 26.112653431809058 56 20.353688767850283 21.910024091337593 31.694074927551412 26.04221221326071 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 1.5 9 1.0 10 1.5 11 2.0 12 9.5 13 37.0 14 57.0 15 82.5 16 108.0 17 146.0 18 184.0 19 428.0 20 672.0 21 895.5 22 1186.5 23 1254.0 24 1385.5 25 1517.0 26 1716.0 27 1915.0 28 2355.5 29 3155.0 30 3514.0 31 3518.5 32 3523.0 33 3585.0 34 3647.0 35 3085.0 36 2523.0 37 2439.5 38 2131.0 39 1906.0 40 1720.5 41 1535.0 42 1631.0 43 1727.0 44 1782.0 45 1837.0 46 1982.0 47 2489.5 48 2852.0 49 2662.0 50 2472.0 51 2659.5 52 2847.0 53 3109.0 54 3826.5 55 4282.0 56 5028.0 57 5774.0 58 6583.5 59 7393.0 60 8147.0 61 8901.0 62 9202.5 63 9651.0 64 9798.0 65 8266.0 66 6734.0 67 5883.0 68 5032.0 69 4419.0 70 3806.0 71 3165.0 72 2244.0 73 1964.0 74 1697.0 75 1430.0 76 1181.5 77 933.0 78 802.5 79 546.5 80 421.0 81 333.5 82 246.0 83 191.0 84 136.0 85 116.0 86 96.0 87 66.0 88 27.0 89 18.0 90 13.5 91 9.0 92 8.0 93 7.0 94 4.5 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.04238754325259516 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 8.650519031141869E-4 21 0.0 22 0.0 23 0.0 24 0.010380622837370242 25 0.023356401384083045 26 0.05968858131487889 27 0.05968858131487889 28 0.0994809688581315 29 0.16695501730103807 30 0.25951557093425603 31 0.31141868512110726 32 0.305363321799308 33 0.3304498269896194 34 0.35986159169550175 35 0.39878892733564014 36 0.4299307958477509 37 0.4420415224913494 38 0.4887543252595156 39 0.5233564013840831 40 0.6029411764705882 41 0.6150519031141869 42 0.6245674740484428 43 0.6704152249134948 44 0.6600346020761246 45 0.7006920415224913 46 0.7517301038062284 47 0.7846020761245674 48 0.8347750865051903 49 0.7975778546712803 50 0.865916955017301 51 0.8339100346020761 52 0.860726643598616 53 0.8598615916955018 54 1.568339100346021 55 0.9117647058823529 56 0.8961937716262975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 115600.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.740484429065745 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15661627314421 28.174740484429066 2 12.196364871751317 9.937716262975778 3 5.97078308136572 7.297577854671281 4 3.3357397655851875 5.43598615916955 5 2.174282316969594 4.429065743944636 6 1.4799558348904367 3.6176470588235294 7 1.0446747069814846 2.9792387543252596 8 0.7835060302361134 2.5536332179930796 9 0.5754204178698828 2.1098615916955015 >10 3.0193647018855105 21.61764705882353 >50 0.17411245116358076 4.846885813148789 >100 0.08493290300662477 5.33044982698962 >500 0.002123322575165619 0.6773356401384083 >1k 0.002123322575165619 0.9922145328719724 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1147 0.9922145328719724 No Hit GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 783 0.6773356401384083 No Hit GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 334 0.2889273356401384 No Hit GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 318 0.2750865051903114 No Hit GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 273 0.23615916955017302 No Hit GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 265 0.2292387543252595 No Hit GCAGGAGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCC 210 0.18166089965397925 No Hit GCAGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 204 0.17647058823529413 No Hit GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 198 0.171280276816609 No Hit GCAGGAGGGGAGTCGGAGGCTTCGACGGCAGTACCGGCCTGGCATCCGTAGAAGCC 187 0.16176470588235295 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 166 0.14359861591695502 No Hit GCAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 165 0.14273356401384082 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA 164 0.14186851211072665 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 159 0.1375432525951557 No Hit GCAGGAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCT 156 0.13494809688581313 No Hit GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 151 0.13062283737024222 No Hit GCAGGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTCTTC 149 0.12889273356401384 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCA 145 0.12543252595155707 No Hit GCAGGAGGGGAGCCCGCTCCCCGAGCAGCCTTAGCCTTCCCTAGCTAATCCAACTG 142 0.12283737024221453 No Hit GCAGGAGGGGGGCCAGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA 142 0.12283737024221453 No Hit GCAGGAGGGCCTGCTGGCATATGTGTCTGATCTGGTGTACTCCACTCGTCTGGTGT 142 0.12283737024221453 No Hit GCAGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 139 0.12024221453287197 No Hit GCAGGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 135 0.11678200692041521 No Hit GCAGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 133 0.11505190311418687 No Hit GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.11418685121107268 No Hit GCAGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 132 0.11418685121107268 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT 129 0.11159169550173012 No Hit GCAGGAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 128 0.11072664359861592 No Hit GCAGGAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT 125 0.10813148788927336 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGTCTGCCCTTCCA 123 0.10640138408304499 No Hit GCAGGAGGGGTTCCATGCTCCATCTGTGGGTGTCTACAACAGCTTTCTTCTCCCTG 123 0.10640138408304499 No Hit GCAGGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 116 0.10034602076124569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0034602076124567475 0.0 0.0 0.0 0.0 7 0.0034602076124567475 0.0 0.0 0.0 0.0 8 0.0034602076124567475 0.0 0.0 0.0 0.0 9 0.004325259515570935 0.0 0.0 0.0 0.0 10 0.009515570934256055 0.0 0.0 0.0 0.0 11 0.012110726643598616 0.0 0.0 0.0 0.0 12 0.014705882352941176 0.0 0.0 0.0 0.0 13 0.015570934256055362 0.0 0.0 0.0 0.0 14 0.015570934256055362 0.0 0.0 0.0 0.0 15 0.018166089965397925 0.0 0.0 0.0 0.0 16 0.01903114186851211 0.0 0.0 0.0 0.0 17 0.01903114186851211 0.0 0.0 0.0 0.0 18 0.020761245674740483 0.0 0.0 0.0 0.0 19 0.023356401384083045 0.0 0.0 0.0 0.0 20 0.028546712802768166 0.0 0.0 0.0 0.0 21 0.029411764705882353 0.0 0.0 0.0 0.0 22 0.03633217993079585 0.0 0.0 0.0 0.0 23 0.037197231833910036 0.0 0.0 0.0 0.0 24 0.037197231833910036 0.0 0.0 0.0 0.0 25 0.03806228373702422 0.0 0.0 0.0 0.0 26 0.039792387543252594 0.0 0.0 0.0 0.0 27 0.04065743944636678 0.0 0.0 0.0 0.0 28 0.04325259515570934 0.0 0.0 0.0 0.0 29 0.04411764705882353 0.0 0.0 0.0 0.0 30 0.045847750865051905 0.0 0.0 0.0 0.0 31 0.045847750865051905 0.0 0.0 0.0 0.0 32 0.04844290657439446 0.0 0.0 0.0 0.0 33 0.050173010380622836 0.0 0.0 0.0 0.0 34 0.054498269896193774 0.0 0.0 0.0 0.0 35 0.05709342560553633 0.0 0.0 0.0 0.0 36 0.06055363321799308 0.0 0.0 0.0 0.0 37 0.06401384083044982 0.0 0.0 0.0 0.0 38 0.06660899653979238 0.0 0.0 0.0 0.0 39 0.07006920415224914 0.0 0.0 0.0 0.0 40 0.0726643598615917 0.0 0.0 0.0 0.0 41 0.0726643598615917 0.0 0.0 0.0 0.0 42 0.07698961937716263 0.0 0.0 0.0 0.0 43 0.078719723183391 0.0 0.0 0.0 0.0 44 0.08044982698961937 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATATT 15 0.00799178 50.651848 50 TACACCT 15 0.00799178 50.651848 48 ATGATCC 15 0.008230917 50.275665 47 CCTACAC 15 0.008230917 50.275665 46 TATGATC 15 0.008230917 50.275665 46 ATCCGTA 35 5.0116796E-8 50.275665 44 GCCTACA 15 0.008230917 50.275665 45 AGCCTAC 15 0.008230917 50.275665 44 ATAAACT 15 0.008230917 50.275665 46 GCTCCCA 15 0.008230917 50.275665 44 ATCCTCT 25 2.0143894E-5 50.25371 42 TAGCCTA 15 0.008245148 50.25371 43 CCACTCG 20 4.0652114E-4 50.25371 42 CCTGTAT 15 0.008302254 50.166084 41 GGCATCC 35 5.0986273E-8 50.166084 41 TACCTGT 15 0.0083165765 50.144226 39 GTACTCC 20 4.11808E-4 50.122387 37 GTTTGGT 15 0.008345276 50.100567 36 GCAAAAG 15 0.008345276 50.100567 36 GTAGGAT 15 0.008345276 50.100567 33 >>END_MODULE