FastQCFastQC Report
Fri 17 Jun 2016
SRR1526974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72877
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2990.4102803353595785No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.3951864099784569No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.3732316094240981No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2450.3361828834886178No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1840.2524802063751252No Hit
AGATGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.22640888071682425No Hit
AGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.16054447905374808No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1070.1468227287072739No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1060.1454505536726265No Hit
AGATGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.13447315339544713No Hit
AGATGGGGGGAGGGTGCAGCTTGCTGCTGCACAGAGTTGAAGTCTGGCCTCACTGCACATCCTG860.11800705297967809No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT840.11526270291038325No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.11251835284108841No Hit
AGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.10428530263320387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTTC150.00452268558.46985657
GTGGACG150.00452268558.46985658
GTCCCGA150.00452268558.46985658
CAAAGTT150.00452268558.46985656
ATGGGGC650.058.107443
GGGTGAT150.00463551258.107447
GGGAATA150.00463551258.107447
GATGGAG700.058.107442
GGATGGA150.00466086658.027518
GGCAACT150.00466086658.027518
GGACGCA258.564428E-658.027518
GGATTCT150.00466086658.027518
GTTCTTA150.004673582557.98763349
CATTGGA150.004673582557.98763323
TGTTGGA150.004673582557.98763353
GCATTGG150.004673582557.98763322
TCAATGG150.004673582557.98763326
AGACACG150.004673582557.98763355
CAAGCGA150.004673582557.98763350
GCTCAAT150.004673582557.98763324
Produced by FastQC (version 0.11.5)