FastQCFastQC Report
Fri 17 Jun 2016
SRR1526973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97108
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC16681.7176751657947853No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9600.9885900234790131No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9490.9772624294599828No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6930.7136384231989126No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC5030.5179799810520245No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4860.5004736993862504No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4390.45207397948675704No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.44589529184001314No Hit
AGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG3990.41088272850846486No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC3360.34600650821765455No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT3120.32129175763067924No Hit
AGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG3060.3151130699839354No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.29554722576924664No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC2570.26465378753552743No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA2550.26259422498661283No Hit
AGATGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2540.26156444371215554No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2390.24611772459529596No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2190.22552209910614984No Hit
AGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2040.21007537998929027No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1870.1925690983235161No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT1860.19153931704905877No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1750.18021172303002841No Hit
AGATGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.18021172303002841No Hit
AGATGGGGGCTGGAGGCCAGTAAGGGACCTCATGGAGGGTAGTGGCAGATTTGGAACCATGTCA1620.16682456646208346No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC1570.16167566008979692No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.1554969724430531No Hit
AGATGGGGGGAGGGTGCAGCTTGCTGCTGCACAGAGTTGAAGTCTGGCCTCACTGCACATCCTG1500.15446719116859578No Hit
AGATGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.15137784734522386No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC1450.14931828479630926No Hit
AGATGGGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG1240.12769287803270585No Hit
AGATGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1230.12666309675824855No Hit
AGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.12254397166041933No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT1180.12151419038596203No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC1180.12151419038596203No Hit
AGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.12151419038596203No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTCC1170.12048440911150472No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCAAAGGTCC1100.11327594019030357No Hit
AGATGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.11224615891584629No Hit
AGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTGG1090.11224615891584629No Hit
AGATGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1070.11018659636693168No Hit
AGATGGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGCTCTTCCAGAGGTCC1030.10606747126910245No Hit
AGATGGGGGGGGCTGGCGAGATGGCTTAGTGGTTAAGAGCACCCGACTGCTCTTCTGAAGGTCC1020.10503768999464513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGG150.004606561758.214153
GTGGGTC201.9649946E-458.214157
TGGGTCC201.9649946E-458.214158
CTGAACA150.004606561758.214158
AAGCCCC201.9649946E-458.214158
TCTACTG150.004606561758.214158
TATCTGT150.004606561758.214157
GATATTT150.004606561758.214154
AAAGGAC150.004606561758.214156
GCGCTAG150.004606561758.214153
GAACCGA258.501853E-658.12370740
TTGATTC150.00463501158.12370347
GTTTGAT303.671048E-758.12370348
TAGAGTT150.00463501158.12370344
AACCGAC303.671048E-758.12370341
CAAACGT201.980162E-458.12370335
ATCTATG150.00463501158.12370349
CGTATAC201.980162E-458.12370339
CTACTAC150.00463501158.12370351
CACGGGA150.00463501158.12370336
Produced by FastQC (version 0.11.5)