SRR1526971_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82439
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1684	2.042722497846893	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	958	1.1620713497252515	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	789	0.9570712890743458	No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	711	0.8624558764662357	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	513	0.6222782906148788	No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	475	0.5761836024211842	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	472	0.572544548090103	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	445	0.5397930591103726	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC	292	0.3542012882252332	No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	286	0.34692317956307084	No Hit
AGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	263	0.31902376302478197	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	242	0.2935503827072138	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	228	0.2765681291621684	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	211	0.2559468212860418	No Hit
AGATGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	180	0.21834325986486977	No Hit
AGATGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.20014798820946395	No Hit
AGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	163	0.19772195198874318	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC	149	0.18073969844369778	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	146	0.1771006441126166	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC	141	0.17103555356081465	No Hit
AGATGGGGGGAGGGTGCAGCTTGCTGCTGCACAGAGTTGAAGTCTGGCCTCACTGC	140	0.16982253545045428	No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	137	0.16618348111937312	No Hit
AGATGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	134	0.16254442678829195	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGGGCGCTTGCCTAGCAAGCGC	128	0.15526631812612962	No Hit
AGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125	0.15162726379504846	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA	124	0.15041424568468806	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGGGCGCTTGCCCAGCAAGCGC	119	0.14434915513288613	No Hit
AGATGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	114	0.1382840645810842	No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGGGCGCCTGCCCAGCAAGCGC	114	0.1382840645810842	No Hit
AGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	112	0.13585802836036343	No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	109	0.13221897402928226	No Hit
AGATGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.12736690158784073	No Hit
AGATGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	102	0.12372784725675953	No Hit
AGATGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	97	0.11766275670495761	No Hit
AGATGGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGCTCTTCC	92	0.11159766615315567	No Hit
AGATGGGGGGTTGCCTTGTGGGGTTCCTGTTGCTGAGACTCCTTGGATGGAGCCAC	92	0.11159766615315567	No Hit
AGATGGGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA	90	0.10917162993243489	No Hit
AGATGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	90	0.10917162993243489	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC	89	0.1079586118220745	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTTCC	89	0.1079586118220745	No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC	86	0.10431955749099335	No Hit
AGATGGGGGGGACCACTGGGCCTTAGTGGGGCTTACACGGGAGGGACTGTCCGAGG	85	0.10310653938063297	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAGC	30	9.599335E-7	50.554462	48
CTGCTCG	15	0.008282194	50.183872	47
GATTGTT	15	0.008302227	50.153236	45
TGCCCTG	15	0.008302227	50.153236	43
TGCCCCG	30	1.0142594E-6	50.153236	43
TGCCTCG	35	5.084803E-8	50.153236	43
GTCCCAA	15	0.008302227	50.153236	44
TCCCAAG	20	4.098646E-4	50.153233	45
CGCCCGA	20	4.111021E-4	50.122635	40
CGTGCCC	20	4.111021E-4	50.122635	41
GCCCGAC	20	4.111021E-4	50.122635	41
CTCGTGG	15	0.0083424	50.09207	38
GGCTCGT	15	0.008362541	50.061546	36
AGGCAAC	15	0.008362541	50.061546	34
AACAGCT	15	0.008362541	50.061546	35
TAAAGAG	15	0.008362541	50.061546	34
ACAGCTG	15	0.008362541	50.061546	36
CGGACCC	15	0.008402931	50.00061	31
CGGAAGG	15	0.008402931	50.00061	31
CCCGGAA	15	0.00842318	49.970196	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content