FastQCFastQC Report
Fri 17 Jun 2016
SRR1526970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614980
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT331915.3970860841002954No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT21930.3565969625028456No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC14880.24195908810042602No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT14510.23594263228072457No Hit
GTGGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT12060.19610393833945822No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9630.15659045822628379No Hit
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTCCATCTTGCTAGG9530.1549643890858239No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC8070.1312237796351101No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT7990.1299229243227422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA150.004700709657.95469331
CACATAT150.004720455557.89336426
GCACATA150.004723498657.8839425
TAGGGTA89950.057.4778635
AGGGTAC87300.057.4602366
GTAGGGT110150.057.136374
GGTACCT86650.056.6020288
GGGTACC87300.056.5291677
TGATCCT84200.056.4241418
TGGTAGG621550.056.4147642
GTACCTG86900.056.363489
GTTGATC85700.056.33963816
TTGATCC85450.056.33508717
ACCTGGT86900.056.3277811
GTACGCT1900.056.3056910
TACCTGG87000.056.29869510
ATGCTTG74100.056.25452836
TATGCTT73600.056.2452735
ATATGCT73500.056.23374634
GGTAGGG618200.056.2321133