FastQCFastQC Report
Fri 17 Jun 2016
SRR1526969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971042
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT922939.504532244743276No Hit
GTGGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT56260.5793776170340726No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC55530.5718599195503388No Hit
GTGGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG51340.528710395636852No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC48090.4952411945106391No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC32580.3355158685206201No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC27270.2808323429882539No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC23240.23933053359174988No Hit
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTCCATCTTGCTAGG21900.22553092451201906No Hit
GTGGTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG19760.20349274284737429No Hit
GTGGTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG19030.1959750453636403No Hit
GTGGTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG18840.19401838437472324No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT15080.15529709322562774No Hit
GTGGTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT14180.14602869906759955No Hit
GTGGTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG14030.14448396670792818No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC13050.13439171529140861No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC11570.11915035600931782No Hit
GTGGTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG11530.11873842738007213No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT10700.11019090832322391No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA10390.10699846144656977No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC10220.10524776477227557No Hit
GTGGTAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC9950.1024672465248671No Hit
GTGGTAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT9930.10226128221024425No Hit
GTGGTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA9850.10143742495175286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG303.8060352E-757.9003525
TCGATCA150.0047296257.86755816
TAGGGTA119250.056.977835
AGGGTAC116400.056.955256
CATGCAT99150.056.42755558
GTAGGGT150100.056.3766634
TAGCATA106850.056.2590830
GTTGATC112600.056.19730816
ATATGCT105850.056.19238734
CATATGC107100.056.15963733
GTAGCAT108150.056.14329
ATTAAGC102650.056.140251
TATGCTT105450.056.08984435
GCATATG107300.056.08200532
GGTAGGG984800.056.051293
ACCTGGT113900.056.03855511
TAGGGGA145250.056.0268175
GCCAGTA110750.056.0181125
GTAGGGG604400.056.0131344
TGATCCT112450.055.98924318
Produced by FastQC (version 0.11.5)