FastQCFastQC Report
Fri 17 Jun 2016
SRR1526968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970114
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT863268.898541820858167No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC51030.5260206532428148No Hit
GTGGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT50880.5244744432097671No Hit
GTGGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG47400.4886023704430613No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC44760.4613890738614224No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC30140.31068513597371034No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC24360.2511045093669404No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC21260.21914950201728867No Hit
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTCCATCTTGCTAGG21160.21811869532859024No Hit
GTGGTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG18970.19554402884609437No Hit
GTGGTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG18450.19018383406486247No Hit
GTGGTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG17580.18121581587318605No Hit
GTGGTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG13930.14359137173569292No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT13310.13720037026576257No Hit
GTGGTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT13190.13596340223932446No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC12450.12833543274295597No Hit
GTGGTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG11170.11514110712761592No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC10890.11225484839926028No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT10810.11143020304830153No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC10000.10308066886984416No Hit
GTGGTAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC9840.10143137816792666No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA9760.1006067328169679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA351.6329068E-858.0359348
CACGATA150.004675525758.03592746
TAGGATA150.004759571557.775345
TAGGGTA122350.057.2322965
AGGGTAC118500.057.0927626
GTAGGGT155200.056.658554
CATGCAT102850.056.38109658
CTCAAAG105950.056.17331344
CCAGTAG111450.056.1053726
TGCCAGT111850.056.06994624
GCCAGTA111650.056.06578425
TAGGGGT68750.056.052585
GTCTCAA106100.056.0517142
TCAAAGA105800.056.0059145
GGTAGGG979650.055.991333
GGTACCT117900.055.9722638
TCTCAAA106000.055.96674343
GTACCTG117700.055.957669
ATTAAGC106700.055.94764751
TAGCATA109750.055.91702730
Produced by FastQC (version 0.11.5)