FastQCFastQC Report
Fri 17 Jun 2016
SRR1526967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909597
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9942910.931104654039096No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC92761.0197922816368128No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC71310.7839735619180802No Hit
GTGGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC51270.563656212586453No Hit
GTGGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC44010.48384064591242054No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT19640.21591979744876028No Hit
GTGGTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC19290.2120719395512518No Hit
GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTCCATC17790.19558111999050126No Hit
GTGGTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT17300.19019411893398946No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC17200.18909473096327276No Hit
GTGGTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC16430.18062944358875416No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC14300.1572124798124884No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC13830.15204535635011987No Hit
GTGGTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT13470.14808755965553974No Hit
GTGGTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC13160.14467945694631798No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA13130.14434964055510296Illumina Single End Adapter 1 (95% over 21bp)
GTGGTAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC11290.12412090189391566No Hit
GTGGTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC10470.11510592053403869No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT10380.11411647136039367No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC10330.11356677737503532No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC10220.11235745060724696No Hit
GTGGTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA10210.11224751181017527No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT10080.11081830744824356No Hit
GTGGTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA9980.10971891947752685No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC9920.10905928669509683No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC9710.10675057195659178No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9230.1014735096971516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA251.9386767E-550.62750249
ATGCGTT204.1599874E-450.05695733
TAACATA150.00842928250.0017130
CCCGATA252.0996764E-549.9493425
AACGCAT150.00855312949.81752418
AGGGTAC129950.049.3683556
TAGGGTA133350.049.36145
GATTAAG117100.049.2090150
GTAGGGT159350.049.0936434
TATGCTT120400.048.956935
ATATGCT120450.048.9257634
AGATTAA117600.048.8839549
CATATGC121800.048.86512433
AAGATTA117950.048.8165648
GCATATG122300.048.7515132
ATGCTTG120650.048.7443536
TAGCATA121450.048.7254230
GTAGCAT122850.048.7034929
GGTACCT129100.048.6126868
GGGTACC129850.048.6005177
Produced by FastQC (version 0.11.5)