##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526967_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 909597 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.726337048165284 38.0 38.0 39.0 35.0 39.0 2 37.13201230874772 38.0 38.0 39.0 34.0 39.0 3 37.57672463739436 38.0 38.0 39.0 35.0 39.0 4 37.523282288749854 38.0 38.0 39.0 35.0 39.0 5 35.419341752446414 36.0 35.0 39.0 30.0 39.0 6 37.808687803499794 38.0 38.0 39.0 35.0 40.0 7 38.081644948257306 38.0 38.0 39.0 36.0 40.0 8 38.071968135339056 38.0 38.0 39.0 36.0 40.0 9 38.05217475431427 38.0 38.0 39.0 36.0 40.0 10 37.12356351219276 38.0 37.0 39.0 33.0 39.0 11 36.99605649534904 39.0 36.0 39.0 33.0 39.0 12 36.73192743599638 38.0 35.0 39.0 32.0 39.0 13 36.55707197802983 38.0 35.0 39.0 32.0 40.0 14 36.62893567151167 38.0 35.0 39.0 32.0 40.0 15 36.59862444577104 38.0 35.0 39.0 32.0 39.0 16 36.308899435684154 38.0 35.0 39.0 32.0 39.0 17 35.90245570291019 38.0 35.0 39.0 31.0 39.0 18 35.92393554508205 38.0 35.0 39.0 30.0 39.0 19 35.60674232654681 38.0 35.0 39.0 30.0 39.0 20 35.498666992085504 38.0 35.0 39.0 30.0 39.0 21 35.19777769715599 38.0 34.0 39.0 29.0 39.0 22 35.13475308295872 38.0 34.0 39.0 29.0 39.0 23 35.10946606024426 38.0 34.0 39.0 29.0 39.0 24 34.76542688685209 37.0 33.0 39.0 29.0 39.0 25 34.44395155217091 36.0 33.0 38.0 28.0 39.0 26 33.87317900125 36.0 33.0 38.0 26.0 39.0 27 33.75057415536771 36.0 33.0 38.0 26.0 39.0 28 33.04320264908525 36.0 32.0 38.0 23.0 39.0 29 33.11537966813875 36.0 32.0 38.0 23.0 39.0 30 32.35767268361703 35.0 31.0 38.0 23.0 39.0 31 31.460666646877684 35.0 30.0 38.0 17.0 39.0 32 31.491531964155556 35.0 30.0 38.0 17.0 39.0 33 31.10777300276936 35.0 29.0 38.0 15.0 39.0 34 31.05660308906032 35.0 29.0 38.0 15.0 39.0 35 30.73068842575338 35.0 29.0 38.0 11.0 39.0 36 30.772225502063 35.0 30.0 38.0 4.0 39.0 37 30.386468952734013 35.0 29.0 38.0 4.0 39.0 38 30.357308786198722 35.0 29.0 38.0 4.0 39.0 39 30.146064685789423 35.0 29.0 38.0 4.0 39.0 40 29.614218164747683 34.0 28.0 38.0 4.0 39.0 41 29.28695675117662 35.0 27.0 38.0 4.0 39.0 42 28.963148515221576 34.0 27.0 38.0 4.0 39.0 43 28.610856236333234 34.0 26.0 38.0 4.0 39.0 44 28.570256937962636 34.0 26.0 38.0 4.0 39.0 45 27.943472768709658 33.0 24.0 37.0 4.0 39.0 46 27.289122545478932 33.0 21.0 37.0 4.0 39.0 47 26.744616571954392 33.0 19.0 36.0 4.0 39.0 48 26.585227303959886 33.0 17.0 37.0 4.0 39.0 49 26.245313034233842 33.0 13.0 36.0 4.0 39.0 50 25.764420946858884 32.0 4.0 36.0 4.0 39.0 51 24.449099986037773 32.0 4.0 36.0 4.0 38.0 52 24.216476087761944 31.0 4.0 36.0 4.0 39.0 53 24.091337152607142 31.0 4.0 36.0 4.0 39.0 54 23.583125274159876 31.0 4.0 36.0 4.0 39.0 55 23.225041419441798 31.0 4.0 36.0 4.0 39.0 56 13.59948306777617 16.0 4.0 21.0 4.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6118499832113997 1 2 -1.0601034995627785 1 3 -0.7691329499928159 1 4 -0.9384535451265492 1 5 -2.1293484539434715 1 6 -0.7130902212053627 1 7 -0.8354440409402386 1 8 -0.8095553827831523 1 9 -0.7664130731581906 1 10 -0.4472041555360846 1 11 -0.6462105923348531 1 12 -0.7504477380005596 1 13 -0.216530488465267 1 14 -0.5518324519446551 1 15 -0.6512967381875114 1 16 -0.12225328731863527 1 17 -0.04948994443407173 1 18 -0.39760285313049337 1 19 -0.17490795230678202 1 20 -0.31883351596091103 1 21 0.15250603004935925 1 22 -0.23994211846591895 1 23 -0.42608367569405914 1 24 -0.09563673683038587 1 25 -0.44222049442802813 1 26 0.17619377384429669 1 27 -0.49043317099364714 1 28 0.5956680103626795 1 29 0.5468980410113176 1 30 0.6089276616210171 1 31 0.32246971186982165 1 32 0.986680066523153 1 33 0.6564741369709388 1 34 -0.24981900956197123 1 35 -0.7700216936108539 1 36 0.8324957929204082 1 37 1.1722139298962908 1 38 1.2742828687821088 1 39 1.0710769576170165 1 40 1.1733210851247726 1 41 1.3344886448183892 1 42 1.2415830132280945 1 43 1.6956278480911955 1 44 1.5898240427182841 1 45 1.4825210727329257 1 46 1.5413691679195303 1 47 2.0077661847975463 1 48 1.4624428867642507 1 49 2.0763492473847727 1 50 1.8257666712775027 1 51 2.410070290492822 1 52 2.7032847145924066 1 53 2.4647930505796047 1 54 3.124009500284874 1 55 2.7832040359043546 1 56 2.2617006022443977 2 1 -0.3507211338852798 2 2 -0.7198515344063594 2 3 -0.4501551953159719 2 4 -0.49804157204980726 2 5 -0.9690740964138485 2 6 -0.38972280429227624 2 7 -0.214175071848409 2 8 -0.15718314524928445 2 9 -0.2304607930817255 2 10 -0.3512692885144588 2 11 -0.2881917786647321 2 12 -0.3121235843754846 2 13 -0.0498616378870409 2 14 -0.13006234794678306 2 15 -0.04178886081851374 2 16 -0.2417074259038401 2 17 -0.40628431168936885 2 18 -0.1539773269898035 2 19 -0.513674857602183 2 20 -0.3401892591387323 2 21 -0.5855483377009705 2 22 -0.40480414621324456 2 23 -0.18463186952640598 2 24 -0.36650728765656027 2 25 -0.7915910183192949 2 26 -0.13676657651335944 2 27 -0.19414916931058457 2 28 -0.3026053891577334 2 29 -0.12881875516130492 2 30 -0.18676013220608212 2 31 0.001398313977148291 2 32 0.2931774946033059 2 33 -0.14859282903720583 2 34 0.06327528737137911 2 35 0.6849147472830346 2 36 0.31045648542420423 2 37 0.6927259552413112 2 38 0.6974928984687452 2 39 0.600886622444829 2 40 0.7708611271432311 2 41 0.8233543357133719 2 42 0.470081311960989 2 43 0.7924464713242294 2 44 0.9585906131472477 2 45 0.7072091737635873 2 46 0.7690303005543022 2 47 1.0644241303773825 2 48 0.5926345905210155 2 49 0.9036848316029555 2 50 0.8674735583154067 2 51 1.0118820635232595 2 52 1.231687940375373 2 53 1.1103194533423988 2 54 1.1538286287294497 2 55 1.0557736699244558 2 56 0.5757339811486872 3 1 -0.47175982451099685 3 2 -0.9450090936201576 3 3 -0.5252519079246198 3 4 -0.6152354829234881 3 5 -1.9688759262778106 3 6 -0.43705755551898307 3 7 -0.257659461065721 3 8 -0.19447122880450962 3 9 -0.2612050754546331 3 10 -0.4391856551347928 3 11 -0.69868307313439 3 12 -0.32035880953583984 3 13 -0.28621162309405435 3 14 -0.5157572552534049 3 15 -0.245890145295931 3 16 -0.11453941670428236 3 17 -0.5514788254468428 3 18 -0.5510586437326666 3 19 -0.5712781712301478 3 20 -0.026611534578478313 3 21 -0.07265053208633532 3 22 0.41678551321323454 3 23 0.03392048367417999 3 24 0.20430643799781478 3 25 0.02248492270974367 3 26 0.3305297687827533 3 27 0.28990092586690963 3 28 0.40528903919120296 3 29 0.5282687578872256 3 30 0.3234163229488516 3 31 0.6516866375694441 3 32 0.6798841860551939 3 33 0.4311315749172664 3 34 0.3797306655972257 3 35 0.6915729847411001 3 36 0.6647707330196475 3 37 0.5718589190292072 3 38 0.8253991612032685 3 39 0.5301496528290564 3 40 0.24769615469167405 3 41 0.5522506117894928 3 42 0.6023598777674302 3 43 0.30024767597536695 3 44 0.2633399977033761 3 45 0.9108862066821573 3 46 0.49752884079400417 3 47 0.969217335653731 3 48 0.8585817042148491 3 49 0.95858493710762 3 50 0.9664455856238092 3 51 0.6881460261444658 3 52 1.2265815704615086 3 53 1.1464227027744052 3 54 1.3459015441749962 3 55 1.4372148971279124 3 56 0.581205988919189 4 1 -0.3542350249996389 4 2 -0.5893891906691024 4 3 -0.5129709184326003 4 4 -0.5753752876944915 4 5 -1.5946493203892729 4 6 -0.29961679722514134 4 7 -0.11696959917382799 4 8 -0.07488781535113276 4 9 -0.0658855944063177 4 10 -0.26373123307369184 4 11 0.023025123994862895 4 12 0.1228511577751803 4 13 9.084664895837591E-4 4 14 0.0434957619733467 4 15 0.25537669405300534 4 16 0.24006531810778853 4 17 0.02856393038118199 4 18 0.15210788908638762 4 19 0.27849956633050965 4 20 0.20688844845492582 4 21 0.13324479531246425 4 22 0.33687566240619304 4 23 0.21251372644358213 4 24 0.7884890438411105 4 25 0.36088655602761577 4 26 0.5284790725866628 4 27 0.6538033566149082 4 28 0.5955072296469268 4 29 0.7519450804335719 4 30 1.3601320419367369 4 31 1.280700651979739 4 32 1.5502622018680619 4 33 1.1753120316580947 4 34 1.2401181827052135 4 35 1.5030090376316423 4 36 1.1160539734137238 4 37 1.0530768945746836 4 38 1.4551257727473441 4 39 0.9902131079071559 4 40 1.3904163528615427 4 41 1.6543980175965913 4 42 1.740504749895834 4 43 1.9779897610572021 4 44 1.7507847148701785 4 45 1.6575522700919905 4 46 2.497760717639121 4 47 2.3174313183930444 4 48 2.124866810934755 4 49 2.305520769432384 4 50 2.8132626517596115 4 51 2.8387161038514854 4 52 2.3399438978263696 4 53 2.530410758289115 4 54 2.727624558800205 4 55 2.572551797217269 4 56 1.7456151554067638 5 1 -0.16890938703378566 5 2 -0.3332074621947072 5 3 -0.2769551069767502 5 4 -0.22123826272662228 5 5 -0.5816117538388781 5 6 -0.17461679722514134 5 7 -0.03194286427388704 5 8 -4.5464112678672564E-4 5 9 -0.05284515930443234 5 10 -0.07733275684824292 5 11 -0.18596319526344018 5 12 -0.2472579619237365 5 13 0.12269855736529678 5 14 -0.05625237920528292 5 15 -0.12554973333821806 5 16 -0.1646385326357418 5 17 0.38259768749519196 5 18 -0.5684779838711975 5 19 0.20784140587520739 5 20 0.07596775816669066 5 21 0.16542707454873806 5 22 -0.17086942663364368 5 23 0.1004136025221598 5 24 -0.33645071657746684 5 25 0.04671705905680312 5 26 0.2701487990415927 5 27 0.08425245703715945 5 28 0.6826997209265286 5 29 0.3454340422474118 5 30 0.6557907761023358 5 31 1.0221523685209597 5 32 0.4728572082936182 5 33 0.7463316296019187 5 34 0.7571459043868707 5 35 0.8417734951311822 5 36 1.150769183621179 5 37 0.446283590921297 5 38 0.8054734411886884 5 39 0.9472967089719653 5 40 1.1265411692742475 5 41 1.4863840879105261 5 42 1.0585267115260883 5 43 1.0621415877507943 5 44 1.1887695000730432 5 45 1.2659490940317752 5 46 1.5508805542464295 5 47 1.4194656951141766 5 48 1.3360983374631203 5 49 1.4278931762618292 5 50 1.8081107332723683 5 51 1.49145786537159 5 52 1.612393181835916 5 53 1.3862832569122077 5 54 1.549949347674815 5 55 2.076427210288685 5 56 1.098693684872524 6 1 -0.055079202200950306 6 2 0.004530091035334749 6 3 -0.07441903512751225 6 4 0.007258781307079687 6 5 0.17462437237295347 6 6 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1.5779694778449915 6 48 1.7398666282124182 6 49 1.5939086243002443 6 50 1.6531037114367244 6 51 1.432376763169902 6 52 1.654538352625078 6 53 1.6014855310809146 6 54 1.5649004779638425 6 55 1.5269161905369408 6 56 1.1564171378035741 7 1 0.01579726816108007 7 2 0.14913865677096538 7 3 -0.004301691015442088 7 4 0.028426012689621416 7 5 0.6216585814787692 7 6 0.23424051837340443 7 7 0.13517967083912907 7 8 0.1752557579058589 7 9 0.20826717908269643 7 10 0.3134390318225755 7 11 -0.07948618149728048 7 12 0.1643185774679523 7 13 0.06753944425953762 7 14 -0.18945365117998136 7 15 0.015102880372133143 7 16 -0.12628890236879187 7 17 0.10980474962894249 7 18 -0.08142736067335932 7 19 0.01073183467253358 7 20 0.04542408781044571 7 21 -0.03936056148511824 7 22 0.18655662675673312 7 23 0.13412994286259305 7 24 0.42886617254912807 7 25 0.18731153864010253 7 26 0.7059803495950305 7 27 0.49408351926393124 7 28 1.1813568225914892 7 29 0.9402987641337504 7 30 1.2178242497326934 7 31 0.9652564983693459 7 32 0.9520335639814625 7 33 1.0604332248023667 7 34 1.241496197382876 7 35 1.3489123659338986 7 36 0.9406637283146964 7 37 1.1461094792107573 7 38 0.9028045034154566 7 39 1.112992051265703 7 40 1.4835268566521655 7 41 1.3950946148382535 7 42 1.8621365501457525 7 43 1.4332814817141468 7 44 1.7986357334466838 7 45 1.613829523759243 7 46 1.6872312425544926 7 47 1.8782573191491494 7 48 1.9868561158093208 7 49 2.0312265871073336 7 50 2.1602698347513005 7 51 2.318742827235603 7 52 2.2443082362494593 7 53 2.1672396795705673 7 54 2.411001517240436 7 55 2.325568278561402 7 56 1.875275514469772 8 1 -0.33738233357876624 8 2 -0.26319035006955005 8 3 -0.3691364955034757 8 4 -0.4348749967553047 8 5 0.3797433635014116 8 6 -0.08905617141027733 8 7 -0.09095932450856736 8 8 -0.1620419944514282 8 9 -0.1320905960710519 8 10 0.30231196283885 8 11 0.35475958830891585 8 12 0.3457790328611452 8 13 0.648101914601277 8 14 0.2774544330502593 8 15 0.20842190942580174 8 16 0.541094029293852 8 17 0.7544400929710662 8 18 0.5961651060896855 8 19 0.43062497823505197 8 20 0.512245463512194 8 21 0.7121391019281376 8 22 0.8078626463780907 8 23 0.5404193065638765 8 24 0.6647512064994601 8 25 0.7723119091219957 8 26 1.3244186817013102 8 27 1.1782469159680602 8 28 1.4741762528691638 8 29 1.3368168649331977 8 30 2.021399478840273 8 31 2.142507648833867 8 32 1.8413383034696302 8 33 2.03173743142721 8 34 2.5997143528875277 8 35 3.1993354247270105 8 36 2.7102949332952306 8 37 2.6168548099564966 8 38 2.8320787215015955 8 39 2.4667108224009056 8 40 3.2490354652325237 8 41 2.8521557632690637 8 42 2.8754880870150075 8 43 2.693334547333585 8 44 2.795903626539797 8 45 3.136956264827081 8 46 3.149193787623222 8 47 2.8086035699512024 8 48 2.979249413082414 8 49 3.1051259011640475 8 50 3.237136157001185 8 51 3.954483452073024 8 52 4.306941747676856 8 53 3.648424717146888 8 54 3.468035234245871 8 55 3.5816627057254493 8 56 3.65675268096118 9 1 0.12685681710106422 9 2 0.42790668848511615 9 3 0.0024410794639280198 9 4 0.1186731500131799 9 5 0.6919057773587411 9 6 0.12290136258115325 9 7 0.13664358106044006 9 8 0.18401872449064882 9 9 0.1916195920459174 9 10 0.1819916052303583 9 11 0.19765398205076679 9 12 0.3615175316504846 9 13 0.7377834195370241 9 14 0.23117020879993788 9 15 0.4565128015844877 9 16 0.6451171248423719 9 17 0.560176729216181 9 18 0.5915087455011232 9 19 0.5332511656070054 9 20 0.6876971304892834 9 21 0.7101834304809387 9 22 0.8890695750426403 9 23 0.7227623872120432 9 24 1.0501116088090114 9 25 0.9083707655198339 9 26 0.9620607006992614 9 27 1.0996475492330973 9 28 1.133144403362742 9 29 1.3664313182764687 9 30 1.5768101698441868 9 31 1.855489582154778 9 32 1.4705988734081679 9 33 1.8292788730364506 9 34 2.0758737866740766 9 35 1.8670575331270882 9 36 2.0806618545096143 9 37 2.310093774381901 9 38 2.325559816677604 9 39 2.2984299507302026 9 40 2.232924457596095 9 41 2.4527331519820486 9 42 2.2286264740516977 9 43 2.441704821127587 9 44 2.547160843898709 9 45 2.792385581273347 9 46 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2.1398717113442274 120 44 1.9461510947972656 120 45 2.2536243384516226 120 46 2.351234501857572 120 47 2.3245397128230856 120 48 2.070306163509972 120 49 1.9900242406410946 120 50 2.183537315083843 120 51 2.616558746205385 120 52 2.634650376080522 120 53 2.6791604935844084 120 54 2.9395943624322385 120 55 2.697031688244781 120 56 2.1487017553392285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 420.0 5 75.0 6 163.0 7 408.0 8 702.0 9 977.0 10 969.0 11 1987.0 12 2223.0 13 2931.0 14 4272.0 15 5461.0 16 6562.0 17 8833.0 18 10734.0 19 11095.0 20 13080.0 21 14561.0 22 14124.0 23 17022.0 24 21925.0 25 18627.0 26 20472.0 27 22941.0 28 25938.0 29 29565.0 30 33802.0 31 40145.0 32 48612.0 33 60909.0 34 77957.0 35 97184.0 36 109316.0 37 101484.0 38 59752.0 39 24369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.57552630450628 0.33069590159158396 0.06332474711328204 0.030453046788852644 8 99.38361278786273 0.40425323672018215 0.08555740428952713 0.1265765711275652 9 98.99559914995322 0.5451864946784125 0.18414748509504758 0.27506687027331883 10 59.96193918845378 16.123184223342864 17.43992119587026 6.474955392333088 11 38.978360746572385 34.23900914360975 12.957606500461194 13.825023609356672 12 33.21108139098964 13.318425632450415 18.400456465885444 35.070036510674505 13 25.178073366556834 13.09953748748072 21.87386282056779 39.84852632539465 14 23.30878399994723 14.592946106902287 40.19164531105533 21.906624582095148 15 39.293005583791505 15.333383905179987 24.18620553937623 21.18740497165228 16 44.965187879907255 14.416384398805185 20.13671988803833 20.48170783324923 17 30.662150380882963 15.340419988192574 34.215592179833486 19.78183745109098 18 29.287805478690014 16.09580946287202 34.430082772920315 20.18630228551765 19 42.03246052922338 19.387377047197827 18.346586455320324 20.23357596825847 20 29.05151875953038 33.300901174264034 17.17991932637856 20.46766073982702 21 26.525373324670156 19.314157808348092 34.483293150703005 19.677175716278747 22 26.082429911268395 16.359442698249886 23.17718725985244 34.38094013062928 23 28.623775144377124 16.394403235718674 20.44663735698337 34.53518426292083 24 30.025474387630684 16.548456859009107 31.029167459190955 22.396901294169258 25 40.80605752715485 15.673389677308686 17.87634674636634 25.644206049170126 26 23.188837835548597 16.00832058222075 22.125994559280848 38.6768470229498 27 23.246616663769753 15.672475891460417 20.456780643642453 40.624126801127375 28 24.100732882892796 31.839359992957284 19.329401148843452 24.730505975306468 29 41.60943688537858 19.048185328695492 18.00676296905573 21.335614816870205 30 28.232452244671265 15.903676063819235 34.53822740506789 21.32564428644161 31 29.05085216685667 27.93329966857115 21.98643412989453 21.029414034677647 32 45.70269647485829 15.815893953576577 18.32901690079041 20.15239267077472 33 31.037779647578162 16.350485382290245 20.21314254017651 32.39859242995509 34 25.548312375909994 29.91981028016766 25.73770130156629 18.794176042356057 35 24.451406161376962 22.46305005594631 36.158309295915146 16.92723448676158 36 25.947454285758436 35.954143367102525 19.477368608119175 18.62103373901986 37 27.257422441639417 21.001198895161806 31.93344475919429 19.80793390400449 38 40.55777543641091 21.99195099916727 17.7598199356345 19.690453628787328 39 29.4777385057503 18.372458658487677 17.61252835375319 34.53727448200883 40 26.61450196419233 18.002018922143243 31.375111532733406 24.008367580931026 41 24.007935630272172 19.582738013615312 31.88606133525605 24.523265020856464 42 34.55550197107323 17.55969630871741 21.473858392436036 26.410943327773328 43 21.770924963901013 15.666572615668898 35.407766399451184 27.154736020978902 44 23.585067456247213 15.214141635436409 21.5872162306602 39.613574677656175 45 24.0939879582079 16.832388879050825 33.976447671329915 25.097175491411367 46 23.0141393653616 17.969427262148994 21.644710110349273 37.37172326214014 47 21.629129170704694 29.926180257510733 23.861110341963172 24.583580229821404 48 23.72361657024001 28.9387453416562 24.342845238266737 22.994792849837047 49 26.165280759711873 28.106104416068987 22.167310321295552 23.561304502923587 50 36.551249408355844 15.706420350563711 21.710209090092857 26.032121150987585 51 23.657289002557544 29.621088884653403 23.840129074009226 22.881493038779823 52 22.68176079435932 17.6305559650661 35.4384638662057 24.249219374368877 53 24.110873907366397 14.738444076446111 36.98150475848447 24.169177257703026 54 24.025580875781948 27.720285969615727 24.019772117962464 24.234361036639857 55 23.954583463643036 28.20977755301664 21.48335695543297 26.352282027907346 56 35.29033467018083 16.742268589923416 19.257478602863152 28.7099181370326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 8.0 6 5.0 7 2.0 8 6.5 9 11.0 10 63.5 11 116.0 12 217.0 13 377.0 14 436.0 15 440.0 16 444.0 17 710.0 18 976.0 19 1143.0 20 1310.0 21 1454.5 22 1986.0 23 2373.0 24 2721.0 25 3069.0 26 3815.5 27 4562.0 28 5529.0 29 7131.0 30 7766.0 31 7860.5 32 7955.0 33 7623.0 34 7291.0 35 6983.0 36 6675.0 37 6506.5 38 7013.0 39 7688.0 40 7978.5 41 8269.0 42 8895.0 43 9521.0 44 13478.0 45 17435.0 46 70750.5 47 75138.5 48 26211.0 49 25363.5 50 24516.0 51 28900.0 52 33284.0 53 32431.0 54 35495.5 55 39413.0 56 43470.5 57 47528.0 58 53327.0 59 59126.0 60 70738.0 61 82350.0 62 84916.0 63 74173.0 64 60864.0 65 55258.5 66 49653.0 67 43594.5 68 37536.0 69 33704.5 70 29873.0 71 26897.5 72 21367.5 73 18813.0 74 15814.0 75 12815.0 76 10424.5 77 8034.0 78 6480.5 79 4103.5 80 3280.0 81 2613.0 82 1946.0 83 1442.5 84 939.0 85 674.5 86 410.0 87 305.0 88 145.0 89 90.0 90 56.5 91 23.0 92 22.0 93 21.0 94 30.0 95 39.0 96 19.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.029243720021064276 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 6.596327824300212E-4 21 0.0 22 0.0 23 0.0 24 0.006156572636013531 25 0.020118799864115647 26 0.05782780725969853 27 0.06409431869278373 28 0.09421754909042136 29 0.155673336653485 30 0.23735786287773594 31 0.2874899543424176 32 0.2856209947921992 33 0.30628948864167316 34 0.3294865748237956 35 0.37060368492860024 36 0.40050703773209456 37 0.41369969338069496 38 0.45547643626792966 39 0.49626372998151935 40 0.5666245601073882 41 0.5849843392183571 42 0.6069720986326912 43 0.6411630645219806 44 0.6405034317395506 45 0.6680980698045398 46 0.7007498925348259 47 0.7390085939157671 48 0.7898003181628788 49 0.7628653128803196 50 0.819044038183943 51 0.7881512362068037 52 0.8175048950249395 53 0.8156359354747212 54 1.5827888614408359 55 0.8684065580691229 56 0.8491672685815805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 909597.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.82239411807227 #Duplication Level Percentage of deduplicated Percentage of total 1 70.59669108080921 22.46555727005308 2 12.303911790050142 7.830798603539433 3 5.520161257190778 5.269942413649146 4 3.0277826275618884 3.854051683125074 5 1.960955948337968 3.1201156518094497 6 1.2786098474054297 2.4413055892430298 7 0.9099787601845821 2.027039192196798 8 0.6826029515292581 1.7377648119778748 9 0.4940229048346723 1.414889242290347 >10 2.8118887486075947 16.64667182584022 >50 0.2348670066486093 5.099821087340444 >100 0.1623293538919484 9.468934496501527 >500 0.00809886147400154 1.7907416563741938 >1k 0.006690363826349097 3.277927893993761 >5k 0.0010563732357393313 2.4129798403330134 >10k+ 3.521244119131104E-4 11.141458741732665 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 99429 10.931104654039096 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 9276 1.0197922816368128 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 7131 0.7839735619180802 No Hit GTGGTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 5127 0.563656212586453 No Hit GTGGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 4401 0.48384064591242054 No Hit GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1964 0.21591979744876028 No Hit GTGGTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC 1929 0.2120719395512518 No Hit GTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTCCATC 1779 0.19558111999050126 No Hit GTGGTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT 1730 0.19019411893398946 No Hit GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 1720 0.18909473096327276 No Hit GTGGTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 1643 0.18062944358875416 No Hit GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 1430 0.1572124798124884 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 1383 0.15204535635011987 No Hit GTGGTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 1347 0.14808755965553974 No Hit GTGGTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 1316 0.14467945694631798 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 1313 0.14434964055510296 Illumina Single End Adapter 1 (95% over 21bp) GTGGTAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC 1129 0.12412090189391566 No Hit GTGGTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC 1047 0.11510592053403869 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 1038 0.11411647136039367 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 1033 0.11356677737503532 No Hit GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 1022 0.11235745060724696 No Hit GTGGTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 1021 0.11224751181017527 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT 1008 0.11081830744824356 No Hit GTGGTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 998 0.10971891947752685 No Hit GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 992 0.10905928669509683 No Hit GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC 971 0.10675057195659178 No Hit GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 923 0.1014735096971516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0029683475209350955 0.0 0.0 0.0 0.0 7 0.003078286318006766 0.0 0.0 0.0 0.0 8 0.003298163912150106 0.0 0.0 0.0 0.0 9 0.003847857897508457 0.0 0.0 0.0 0.0 10 0.006486389027228542 0.0 0.0 0.0 0.0 11 0.01110381850423869 0.0 0.0 0.0 0.0 12 0.012313145272027063 0.0 0.0 0.0 0.0 13 0.013192655648600424 0.0 0.0 0.0 0.0 14 0.014841737604675477 0.0 0.0 0.0 0.0 15 0.016051064372463848 0.0 0.0 0.0 0.0 16 0.01638088076367886 0.0 0.0 0.0 0.0 17 0.017260391140252223 0.0 0.0 0.0 0.0 18 0.018139901516825582 0.0 0.0 0.0 0.0 19 0.020118799864115647 0.0 0.0 0.0 0.0 20 0.02231757580554905 0.0 0.0 0.0 0.0 21 0.02352690257333742 0.0 0.0 0.0 2.198775941433404E-4 22 0.025066045732340805 0.0 0.0 0.0 2.198775941433404E-4 23 0.02638531129720085 0.0 0.0 0.0 2.198775941433404E-4 24 0.028584087238634252 0.0 0.0 0.0 2.198775941433404E-4 25 0.029353658818135942 0.0 0.0 0.0 2.198775941433404E-4 26 0.030672924382995986 0.0 0.0 0.0 2.198775941433404E-4 27 0.03199218994785603 0.0 0.0 0.0 2.198775941433404E-4 28 0.036279803033651165 0.0 0.0 0.0 2.198775941433404E-4 29 0.03759906859851121 0.0 0.0 0.0 2.198775941433404E-4 30 0.038698456569227914 0.0 0.0 0.0 2.198775941433404E-4 31 0.040127660931159624 0.0 0.0 0.0 2.198775941433404E-4 32 0.041666804090163004 0.0 0.0 0.0 2.198775941433404E-4 33 0.04342582484330973 0.0 0.0 0.0 2.198775941433404E-4 34 0.046504111161316496 0.0 0.0 0.0 2.198775941433404E-4 35 0.04958239747932326 0.0 0.0 0.0 2.198775941433404E-4 36 0.05244080620318669 0.0 0.0 0.0 2.198775941433404E-4 37 0.05376007176804673 0.0 0.0 0.0 2.198775941433404E-4 38 0.05518927612997844 0.0 0.0 0.0 2.198775941433404E-4 39 0.057168174477268505 0.0 0.0 0.0 2.198775941433404E-4 40 0.05936695041870191 1.099387970716702E-4 0.0 0.0 2.198775941433404E-4 41 0.06255517553378034 1.099387970716702E-4 0.0 0.0 2.198775941433404E-4 42 0.06519370666350043 1.099387970716702E-4 0.0 0.0 2.198775941433404E-4 43 0.06629309463421713 1.099387970716702E-4 0.0 0.0 2.198775941433404E-4 44 0.06783223779322052 1.099387970716702E-4 0.0 0.0 2.198775941433404E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTAA 25 1.9386767E-5 50.627502 49 ATGCGTT 20 4.1599874E-4 50.056957 33 TAACATA 15 0.008429282 50.00171 30 CCCGATA 25 2.0996764E-5 49.94934 25 AACGCAT 15 0.008553129 49.817524 18 AGGGTAC 12995 0.0 49.368355 6 TAGGGTA 13335 0.0 49.3614 5 GATTAAG 11710 0.0 49.20901 50 GTAGGGT 15935 0.0 49.093643 4 TATGCTT 12040 0.0 48.9569 35 ATATGCT 12045 0.0 48.92576 34 AGATTAA 11760 0.0 48.88395 49 CATATGC 12180 0.0 48.865124 33 AAGATTA 11795 0.0 48.81656 48 GCATATG 12230 0.0 48.75151 32 ATGCTTG 12065 0.0 48.74435 36 TAGCATA 12145 0.0 48.72542 30 GTAGCAT 12285 0.0 48.70349 29 GGTACCT 12910 0.0 48.612686 8 GGGTACC 12985 0.0 48.600517 7 >>END_MODULE