FastQCFastQC Report
Fri 17 Jun 2016
SRR1526966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144061
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7130.494929231367268No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4910.3408278437606292No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3140.21796322391209277No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2760.19158550891636184No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.15201893642276537No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1940.13466517655715285No Hit
ATACAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.12217046945391188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT650.058.58043358
AGTGTGA150.00449793658.5804358
AGATACC150.00450421158.5598256
AACACCC150.004650337658.08970355
TTGATTA150.004656770358.06943544
AGGTACA150.004656770358.06943545
ACGATAA201.992593E-458.06943543
TGAGGTA150.004656770358.06943543
AGCTAAC201.992593E-458.06943543
GCTAACT201.992593E-458.06943544
TGTGCTA150.00466320958.04918349
CACGATA201.9960343E-458.0491842
ATAACCC201.9960343E-458.0491847
CACAATA201.9960343E-458.0491841
GATTTGC258.605644E-658.02894237
CTTGTGT150.004689031757.96830731
TAGCGAC150.004689031757.96830730
TAGCATA1200.057.96830730
GTTAAGT150.004689031757.96830731
GCATATG1200.057.96830732
Produced by FastQC (version 0.11.5)