##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526963_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 189214 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.551291130677434 39.0 38.0 40.0 36.0 40.0 2 38.54778187660533 39.0 38.0 40.0 36.0 40.0 3 38.44487722895769 39.0 38.0 40.0 36.0 40.0 4 38.41616370881647 39.0 38.0 40.0 36.0 40.0 5 38.16029469278172 39.0 38.0 40.0 35.0 40.0 6 38.30577547115964 39.0 38.0 40.0 35.0 40.0 7 38.69204710010887 39.0 39.0 40.0 38.0 40.0 8 38.65944908939085 39.0 39.0 40.0 38.0 40.0 9 38.65231959580158 39.0 39.0 40.0 37.0 40.0 10 38.010184235838786 39.0 38.0 40.0 35.0 40.0 11 37.56857843499952 39.0 37.0 40.0 33.0 40.0 12 37.53529337152642 39.0 37.0 40.0 33.0 40.0 13 37.36808587102434 39.0 37.0 40.0 33.0 40.0 14 37.63227351041677 39.0 38.0 40.0 33.0 40.0 15 37.62726859534707 39.0 38.0 40.0 33.0 40.0 16 37.37226632278796 39.0 37.0 40.0 33.0 40.0 17 37.12040335281745 39.0 36.0 40.0 33.0 40.0 18 37.018730115107765 39.0 36.0 40.0 33.0 40.0 19 36.88849133785026 39.0 36.0 40.0 32.0 40.0 20 36.86014776919255 39.0 36.0 40.0 32.0 40.0 21 36.60768759182724 38.0 36.0 40.0 32.0 40.0 22 36.43795385119494 38.0 35.0 40.0 31.0 40.0 23 36.3559990275561 38.0 35.0 40.0 31.0 40.0 24 36.277098946166774 38.0 35.0 40.0 31.0 40.0 25 36.108929571807586 38.0 35.0 39.0 31.0 40.0 26 35.59609754035114 38.0 35.0 39.0 29.0 40.0 27 35.31640893379983 38.0 35.0 39.0 29.0 40.0 28 35.00308116735548 38.0 34.0 39.0 28.0 40.0 29 34.85812360607566 38.0 34.0 39.0 27.0 40.0 30 34.4482332174152 37.0 33.0 39.0 27.0 40.0 31 33.5644085532783 37.0 33.0 39.0 23.0 40.0 32 33.50751001511516 37.0 33.0 39.0 23.0 40.0 33 33.229491475260815 36.0 32.0 38.0 23.0 40.0 34 33.18828945004069 36.0 32.0 39.0 23.0 40.0 35 32.90196814189225 36.0 32.0 39.0 21.0 40.0 36 32.799877387508324 36.0 32.0 39.0 17.0 40.0 37 32.519047216379334 36.0 32.0 39.0 16.0 40.0 38 32.204683585781176 36.0 31.0 39.0 14.0 40.0 39 32.079307028021184 36.0 31.0 39.0 10.0 40.0 40 31.732947879120996 36.0 31.0 39.0 4.0 40.0 41 31.35803376071538 36.0 30.0 39.0 4.0 40.0 42 31.13354191550308 36.0 30.0 39.0 4.0 40.0 43 30.960658302239793 36.0 30.0 39.0 4.0 40.0 44 30.740632299935523 35.0 30.0 39.0 4.0 40.0 45 30.295897766550045 35.0 29.0 38.0 4.0 40.0 46 29.416163708816473 35.0 28.0 38.0 4.0 40.0 47 29.082097519211054 34.0 27.0 38.0 4.0 40.0 48 28.7690022936992 34.0 26.0 38.0 4.0 39.0 49 28.536545921549145 34.0 26.0 38.0 4.0 39.0 50 28.076088450114685 34.0 24.0 38.0 4.0 39.0 51 27.010649317703763 33.0 18.0 37.0 4.0 39.0 52 26.68012409229761 33.0 15.0 37.0 4.0 39.0 53 26.44364053399854 33.0 4.0 37.0 4.0 39.0 54 26.068467449554472 33.0 4.0 37.0 4.0 39.0 55 25.643826566744533 33.0 4.0 37.0 4.0 39.0 56 15.066395721246842 18.0 4.0 22.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6941861522583821 1 2 -0.5468184177872004 1 3 -0.5493773668643627 1 4 -0.6957808606064546 1 5 -0.6771408959822978 1 6 -0.7671667795161596 1 7 -1.1946903181938993 1 8 -0.7993851468306161 1 9 -0.8639070050152782 1 10 -0.7166230093885986 1 11 -0.6931070551095146 1 12 -0.6830088701422383 1 13 -0.5356764906830804 1 14 -0.8160623472288648 1 15 -0.8952881351323967 1 16 -0.6835117706153966 1 17 -0.7459843770539223 1 18 -0.5299252060901196 1 19 -0.3952899618901924 1 20 -0.9091279110727157 1 21 -0.2618164657367288 1 22 -0.8720665484064583 1 23 -0.389148128574071 1 24 -0.34732344640708135 1 25 -0.9955249855909756 1 26 0.12582291079697683 1 27 -0.48640734297854493 1 28 0.4578889917488027 1 29 -0.10197730585147013 1 30 -0.1375096774539486 1 31 0.4178472859554887 1 32 0.9180598740526591 1 33 0.026844435588046167 1 34 0.04349661636800306 1 35 -0.04775260516461799 1 36 1.2020586976335181 1 37 1.3626849852265863 1 38 1.4450141149652609 1 39 1.216355025282148 1 40 1.4067203117806066 1 41 1.4582813372903196 1 42 1.649940227704409 1 43 1.963361188023839 1 44 1.953523210360924 1 45 1.8629699807687956 1 46 2.24880505394772 1 47 2.5528822520284855 1 48 2.568878459615604 1 49 2.7902931868383334 1 50 2.0830862145700237 1 51 2.942823813978883 1 52 2.37823375809122 1 53 1.8198924919813493 1 54 1.6052602994473233 1 55 2.2807549653778416 1 56 1.8172751250308359 2 1 -0.5821532282970523 2 2 -0.6419320838441678 2 3 -0.5342218926025382 2 4 -0.5955064942620609 2 5 -1.1254032424677973 2 6 -0.4703938503288043 2 7 -0.15458057165614036 2 8 -0.20381420353299973 2 9 -0.24390439200247727 2 10 -0.27678762919523336 2 11 -0.8908467990342643 2 12 -0.5213940282295155 2 13 -0.5648769087651289 2 14 -0.39366882750061905 2 15 -0.2285779182523342 2 16 -0.15548720829503537 2 17 -1.0545158638582066 2 18 -0.19630879636971343 2 19 -0.4781877930895675 2 20 -1.1694493116475755 2 21 -1.709425559021284 2 22 -1.3627903529530982 2 23 -1.6328115725249503 2 24 -1.4768635561536172 2 25 -1.7625095688154317 2 26 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2.5350887280334824 3 52 2.6632438693146767 3 53 1.9441341502219949 3 54 1.741129864664714 3 55 1.788338382971368 3 56 0.4013246700257813 4 1 -0.34595257747124464 4 2 -0.2565019862202931 4 3 -0.3620600457308001 4 4 -0.6226140033183611 4 5 -0.5466287597515205 4 6 -0.3727392791984556 4 7 -0.20148914904915216 4 8 -0.15537065960225505 4 9 -0.1562885020288789 4 10 -0.580741702996427 4 11 -0.15501454017750405 4 12 -0.07422757645816347 4 13 -0.008512937499702389 4 14 -0.1703004969302242 4 15 0.18706032083405688 4 16 0.019468277675365186 4 17 -0.09307422328596715 4 18 -0.08072581607868301 4 19 -0.2644283530453606 4 20 0.27783993744679236 4 21 -0.4097386921949848 4 22 0.30749883474260997 4 23 0.17451942765924855 4 24 0.15391559286093326 4 25 -0.044482921796337394 4 26 0.6525416806533784 4 27 0.47152886238427527 4 28 0.3183223377902351 4 29 0.9315402948601843 4 30 0.5687744755515141 4 31 0.8526865285530292 4 32 0.9175515502503941 4 33 0.49339037535167307 4 34 1.191831855915595 4 35 1.0788835620739121 4 36 1.3978586722173247 4 37 0.8462597723659897 4 38 0.9663954848979088 4 39 1.1497010667105414 4 40 0.8867686025418244 4 41 0.9487184957602643 4 42 0.9864505848018759 4 43 1.0332557108348688 4 44 0.5900081512682824 4 45 0.9670175090443074 4 46 2.4830152458399546 4 47 2.110195760733532 4 48 1.6518623203348852 4 49 2.1864998204639505 4 50 2.207943248500637 4 51 2.1662956655483327 4 52 1.5120499990367016 4 53 1.9626361697040586 4 54 2.155806747534754 4 55 1.7686187345988351 4 56 1.0814446510188915 5 1 -0.1981289483022195 5 2 -0.15013303954609114 5 3 -0.24344312468312523 5 4 -0.02557236020549425 5 5 -0.22846407160378845 5 6 0.019855407064966357 5 7 0.15615170265614609 5 8 0.20829996410228802 5 9 0.16168775574387695 5 10 0.1536656834742729 5 11 0.0038322659276417426 5 12 -0.1585501692601241 5 13 3.4329012418510274E-4 5 14 -0.2620660895437581 5 15 -0.44955170102387143 5 16 -0.07050722637588791 5 17 -0.03701618673047591 5 18 -0.9314889590973365 5 19 -0.06148883919572512 5 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7 31 1.36282823458248 7 32 1.2691306926047545 7 33 1.4890373143161995 7 34 0.37206710644815644 7 35 0.6301478021405984 7 36 1.0290525223608853 7 37 1.05270600743129 7 38 0.6766853802392063 7 39 0.326787294366369 7 40 1.0930296009673093 7 41 0.7252304898326933 7 42 0.8060300976295132 7 43 0.9679597953027752 7 44 0.9480705760158976 7 45 0.4311279100816314 7 46 0.5098745586120224 7 47 0.8368790986977785 7 48 1.013629477878645 7 49 1.2132665149161141 7 50 1.181824882287799 7 51 0.6400383718556384 7 52 1.0209876669074056 7 53 0.9424453759650575 7 54 0.7083676440800915 7 55 0.8151131311904791 7 56 1.1992551539363667 8 1 -0.5659450402562456 8 2 -0.2535813154081623 8 3 -0.2032132396256543 8 4 0.11810580071404786 8 5 -0.03880889918999486 8 6 -0.22152390327985927 8 7 0.02971492104694562 8 8 0.19105858479193927 8 9 0.1444463764335282 8 10 0.21688407427887313 8 11 0.41187824293913167 8 12 0.47938086522263745 8 13 0.6899984625379787 8 14 0.2609224162033712 8 15 0.47573565529796724 8 16 0.28006748626778943 8 17 0.7733286408557305 8 18 0.3443731098681795 8 19 0.501729551608868 8 20 -0.13199612812697836 8 21 0.7866999737179654 8 22 0.6042766753305102 8 23 0.887916827320069 8 24 0.717699274790462 8 25 0.7908289304742482 8 26 1.497180836485299 8 27 1.4700214193844587 8 28 1.6370523170628886 8 29 1.6829064150787616 8 30 2.03777767886789 8 31 2.2747705685002657 8 32 2.132173747348567 8 33 2.1850240667027165 8 34 2.599029896239699 8 35 2.477508020336586 8 36 2.202927449304191 8 37 2.141153309204128 8 38 2.3174412734790266 8 39 2.15554240833481 8 40 2.7905748961163503 8 41 2.3692008775202034 8 42 1.7456365655819788 8 43 1.5399449891465338 8 44 2.366247080983751 8 45 2.6608181497075805 8 46 3.1849183926272175 8 47 3.6155660264203604 8 48 3.801570253253672 8 49 3.9371122191826267 8 50 3.133607465572428 8 51 3.9782421513218402 8 52 4.567354314092288 8 53 4.276655389602304 8 54 4.41847869025192 8 55 4.5407121960648205 8 56 3.9221267689763053 9 1 -0.22686458048612934 9 2 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120 35 -0.391514650648368 120 36 -0.2416969235922366 120 37 -0.31664817679320123 120 38 -0.09304961367408282 120 39 -0.16274043919170467 120 40 -0.13439493940787983 120 41 -0.7502821986212815 120 42 -1.195749095178801 120 43 -0.5478812634730161 120 44 -0.5455773935193271 120 45 0.07410321987967805 120 46 -0.4861300610464987 120 47 0.03399493351377458 120 48 -0.4493664330992857 120 49 -0.43645099920818 120 50 -0.39173069407702954 120 51 -0.1746758648082185 120 52 0.23779947825054037 120 53 0.044452430308471236 120 54 0.02100615580091514 120 55 0.04344287492428833 120 56 0.7349482587838878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 67.0 5 15.0 6 43.0 7 30.0 8 95.0 9 117.0 10 142.0 11 243.0 12 297.0 13 430.0 14 543.0 15 685.0 16 898.0 17 1117.0 18 1457.0 19 1649.0 20 1692.0 21 2176.0 22 2251.0 23 2298.0 24 3106.0 25 3611.0 26 3211.0 27 3649.0 28 4158.0 29 4584.0 30 5327.0 31 6283.0 32 7605.0 33 9406.0 34 12203.0 35 16945.0 36 23350.0 37 29379.0 38 27672.0 39 12480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.204075808344 0.7224623970742122 0.040694663185599375 0.0327671313961969 8 99.28465686792852 0.600613302315745 0.045997673680871314 0.06873215607486519 9 98.2115488283108 1.0326931410994957 0.4619108522625176 0.2938471783271851 10 70.54816239813123 17.976999587768347 5.059350787996659 6.415487226103776 11 45.473379348251186 22.81913600473538 13.032333759658377 18.67515088735506 12 41.02074899320346 12.859513566649403 22.197617512446225 23.922119927700912 13 28.687095035251094 13.893263711987485 24.83748559831725 32.58215565444417 14 24.90883338442187 15.966577525975879 33.80405255425074 25.320536535351508 15 32.86596129250478 14.943397423023667 29.261576838923126 22.929064445548427 16 38.46174173158434 16.307461392920185 23.108755166108217 22.122041709387254 17 33.792425507626284 18.837929540097456 24.243449216231358 23.126195736044902 18 35.09042671261112 19.359561131840138 22.258923758284272 23.291088397264474 19 33.56516959633008 24.64141131205936 19.31305294534231 22.480366146268246 20 33.74698088377526 23.811512015686194 21.230795249747636 21.21071185079092 21 31.495555297176743 23.45545255636475 24.386673290559894 20.66231885589861 22 29.052289999682902 20.234232139270876 28.048664475144548 22.664813385901677 23 34.46256619489044 17.9156933419303 24.384559282082723 23.237181181096535 24 36.264125413587884 18.02926035158934 22.05784416325754 23.648770071565238 25 31.46311549075376 17.301938062360566 20.280929170323223 30.95401727656245 26 25.043633709552875 16.902905740609498 26.5462199985191 31.50724055131853 27 24.830088486214027 16.44478999730257 24.24035669517478 34.48476482130862 28 24.301987820170265 24.035322564430874 21.486129703017447 30.176559912381418 29 32.55951845244083 21.441995648283932 21.05976462616006 24.938721273115185 30 30.52759293010639 17.331411859449837 27.263913025049803 24.877082185393974 31 34.66557825437043 17.70489891547489 23.651764606104297 23.977758224050376 32 39.65280538549204 15.958760700750046 23.119980917547906 21.268452996210012 33 36.41308669858286 17.603740874461216 25.173497897878793 20.809674529077135 34 27.838790931989926 20.855362587829777 33.09134296698926 18.214503513191037 35 25.05145997793057 27.55071725659961 29.028202189966894 18.36962057550293 36 26.76704418804598 31.605120421182242 22.557344683741814 19.070490707029965 37 31.214670523606063 27.88620259547252 21.831692364852312 19.067434516069106 38 31.53654987839718 26.658630614173894 21.513078940940325 20.2917405664886 39 33.78399196782882 22.44279999787509 21.42602912193282 22.347178912363276 40 29.42846509649636 19.769259397097134 22.629060556116755 28.173214950289754 41 24.353397851749442 22.65978942890567 23.32872487503988 29.658087844305008 42 23.33106411947411 20.32825596732332 23.97991745734587 32.3607624558567 43 23.03538175046555 17.652567172120246 27.93189678105879 31.38015429635541 44 25.717098087206363 16.882066562026125 24.936550586607783 32.46428476415973 45 27.14368589965352 18.80791318278345 25.420327959593163 28.628072957969863 46 25.536831456486436 21.636184543300587 24.421904533716692 28.405079466496286 47 23.64831395441738 20.704458659792166 27.62440944462495 28.022817941165506 48 26.688231407072667 18.75439643595592 30.438790953467056 24.11858120350436 49 29.93255266326411 17.46598331397641 27.05725032231942 25.54421370044006 50 25.425011861416014 16.75684896819008 28.040387455153187 29.77775171524072 51 22.56720172241052 19.162882905928246 30.748651702159407 27.52126366950182 52 22.234246721398872 18.504638021110992 30.77833457724704 28.482780680243096 53 24.21633436400469 17.067917688452926 32.56317304616697 26.152574901375413 54 23.5948657056431 18.029668871325644 29.924081645811057 28.451383777220197 55 23.75590710773071 18.648859114813906 25.645648208399564 31.94958556905582 56 25.00773275595422 20.380878227758995 22.74656825623687 31.864820760049916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 9.5 7 17.0 8 81.0 9 145.0 10 132.0 11 119.0 12 156.0 13 226.0 14 259.0 15 310.5 16 362.0 17 475.0 18 588.0 19 585.5 20 583.0 21 665.0 22 902.5 23 1058.0 24 1294.0 25 1530.0 26 1842.5 27 2155.0 28 2272.0 29 2426.5 30 2464.0 31 2112.5 32 1761.0 33 1840.0 34 1919.0 35 1830.0 36 1741.0 37 1708.5 38 1710.5 39 1745.0 40 1850.5 41 1956.0 42 2384.5 43 2813.0 44 3744.0 45 4675.0 46 4105.5 47 3960.0 48 4384.0 49 5014.0 50 5644.0 51 6416.0 52 7188.0 53 8711.0 54 11820.5 55 13407.0 56 15330.0 57 17253.0 58 19172.0 59 21091.0 60 22024.5 61 22958.0 62 19011.0 63 12704.5 64 10345.0 65 9218.5 66 8092.0 67 6911.0 68 5730.0 69 5009.0 70 4288.0 71 3769.5 72 2857.5 73 2464.0 74 1947.0 75 1430.0 76 1145.0 77 860.0 78 686.5 79 407.5 80 302.0 81 229.5 82 157.0 83 118.5 84 80.0 85 55.0 86 30.0 87 19.5 88 6.0 89 3.0 90 3.0 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03910915682771888 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.001585506357880495 21 0.0 22 0.0 23 0.0 24 0.008456033908695974 25 0.02959611868043591 26 0.07399029670108978 27 0.07663280729755727 28 0.11257095140951516 29 0.17070618453179998 30 0.2483959960679442 31 0.2959611868043591 32 0.29543268468506556 33 0.31445876097963155 34 0.33771285422854547 35 0.3794645216527318 36 0.4185736784804507 37 0.42755821450844017 38 0.47353789888697456 39 0.5131755578339869 40 0.5940363820858922 41 0.6098914456646971 42 0.6289175219592631 43 0.668026678786982 44 0.6706691893834494 45 0.7002653080638853 46 0.7393744648916042 47 0.7753126090035621 48 0.8255203103364445 49 0.7990952043717695 50 0.8619869565676959 51 0.8297483272907925 52 0.8635724629255763 53 0.8635724629255763 54 1.6346570549747905 55 0.9132516621391652 56 0.8984536027989471 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 189214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.78480450706607 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33996149535932 25.506569281342816 2 12.035573690411196 8.854524506643271 3 5.762765437774776 6.359466001458666 4 3.31599666676245 4.879131565317577 5 2.127812419183357 3.9135581933683556 6 1.449670986465906 3.199551830202839 7 1.0387632539294847 2.6747492257443954 8 0.7241171230711761 2.1309205449913855 9 0.6034309358926467 1.997738010929424 >10 3.2326657279963222 21.788028369993764 >50 0.23562541306284301 6.116355026583657 >100 0.12499640814919112 8.15637320705656 >500 0.004310220970661763 0.9766719164543849 >1k 0.004310220970661763 3.446362319912903 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2907 1.5363556607861997 No Hit ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1844 0.974557907977211 No Hit ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1770 0.9354487511494921 No Hit ATACAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 692 0.3657234665511009 No Hit ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 585 0.30917373978669654 No Hit ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 571 0.3017747101165875 No Hit ATACAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 495 0.2616085490502817 No Hit ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 452 0.23888295792066128 No Hit ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 431 0.22778441341549782 No Hit ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT 415 0.2193283795068018 No Hit ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 359 0.1897322608263659 No Hit ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 332 0.17546270360544147 No Hit ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 315 0.16647816757745199 No Hit ATACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 306 0.16172164850381052 No Hit ATACAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 290 0.15326561459511454 No Hit ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 281 0.14850909552147304 No Hit ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 272 0.14375257644783154 No Hit ATACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 266 0.14058156373207056 No Hit ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 255 0.13476804041984208 No Hit ATACAGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTGTCC 252 0.1331825340619616 No Hit ATACAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 248 0.1310685255847876 No Hit ATACAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 243 0.1284260149883201 No Hit ATACAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 226 0.11944147896033064 No Hit ATACAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 225 0.11891297684103713 No Hit ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 213 0.11257095140951516 No Hit ATACAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC 213 0.11257095140951516 No Hit ATACAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 212 0.11204244929022165 No Hit ATACAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 212 0.11204244929022165 No Hit ATACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 206 0.10887143657446066 No Hit ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 206 0.10887143657446066 No Hit ATACAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 196 0.10358641538152569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0021140084771739936 0.0 0.0 0.0 0.0 6 0.0021140084771739936 0.0 0.0 0.0 0.0 7 0.0021140084771739936 0.0 0.0 0.0 0.0 8 0.002642510596467492 0.0 0.0 0.0 0.0 9 0.0031710127157609904 0.0 0.0 0.0 0.0 10 0.005813523312228482 0.0 0.0 0.0 0.0 11 0.005813523312228482 0.0 0.0 0.0 0.0 12 0.006870527550815479 0.0 0.0 0.0 0.0 13 0.006870527550815479 0.0 0.0 0.0 0.0 14 0.008456033908695974 0.0 0.0 0.0 0.0 15 0.010041540266576469 0.0 0.0 0.0 0.0 16 0.011098544505163465 0.0 0.0 0.0 0.0 17 0.013212552982337459 0.0 0.0 0.0 0.0 18 0.014269557220924456 0.0 0.0 0.0 0.0 19 0.01691206781739195 0.0 0.0 0.0 0.0 20 0.01902607629456594 0.0 0.0 0.0 0.0 21 0.01902607629456594 0.0 0.0 0.0 0.0 22 0.021668586891033435 0.0 0.0 0.0 0.0 23 0.02272559112962043 0.0 0.0 0.0 0.0 24 0.023782595368207426 0.0 0.0 0.0 0.0 25 0.024311097487500925 0.0 0.0 0.0 0.0 26 0.025368101726087923 0.0 0.0 0.0 0.0 27 0.02589660384538142 0.0 0.0 0.0 0.0 28 0.02906761656114241 0.0 0.0 0.0 0.0 29 0.030124620799729405 0.0 0.0 0.0 0.0 30 0.034881139873370895 0.0 0.0 0.0 0.0 31 0.03699514835054488 0.0 0.0 0.0 0.0 32 0.03805215258913188 0.0 0.0 0.0 0.0 33 0.03963765894701238 0.0 0.0 0.0 0.0 34 0.04228016954347987 0.0 0.0 0.0 0.0 35 0.04333717378206687 0.0 0.0 0.0 0.0 36 0.04439417802065386 0.0 0.0 0.0 0.0 37 0.04545118225924086 0.0 0.0 0.0 0.0 38 0.047036688617121354 0.0 0.0 0.0 0.0 39 0.05020770133288235 0.0 0.0 0.0 0.0 40 0.05602122464511083 0.0 0.0 0.0 0.0 41 0.05919223736087182 0.0 0.0 0.0 0.0 42 0.06183474795733931 0.0 0.0 0.0 0.0 43 0.0634202543152198 0.0 0.0 0.0 0.0 44 0.0655342627923938 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAAGT 15 0.007965021 50.707596 49 AATAAAC 20 4.0529273E-4 50.30128 47 ATGTATA 15 0.008239857 50.274426 44 TAACCAA 25 2.0144409E-5 50.274426 45 ACTATGT 15 0.008248557 50.26101 43 CCCAATT 20 4.0689838E-4 50.26101 42 GTATAGT 15 0.008248557 50.26101 42 TACCGAC 15 0.008248557 50.26101 42 CAAGTTA 25 2.0336138E-5 50.19403 40 AGTACCG 15 0.008292164 50.19403 40 GCAAACT 20 4.1120494E-4 50.153927 37 CCGTCAT 15 0.008327171 50.14058 36 TATTATT 20 4.122872E-4 50.12723 35 TATTCGC 15 0.0083535 50.10056 34 TAGGATT 15 0.0083535 50.10056 34 GTATAGG 15 0.008371086 50.073917 33 ATATTCG 15 0.008371086 50.073917 33 ATGGTTA 15 0.0083887 50.047306 31 GTGCAAT 15 0.008432856 49.980896 28 GCAAATG 15 0.008432856 49.980896 28 >>END_MODULE