FastQCFastQC Report
Fri 17 Jun 2016
SRR1526962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455802
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT16510.36221868267361707No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9390.20601050456119105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG454500.057.320031
AAGGGGG447200.057.2011382
AGGGGGG428250.056.611333
TAAGGGA4500.052.794091
CATATGC3550.049.78897533
ATTAAGC3250.049.12191451
GTACCTG4300.049.104719
ATATGCT3550.048.97276334
TAGCATA3800.048.7955630
GTTGATC4300.048.4639416
GTAGCAT3950.048.40420529
CAGTAGC4150.047.45193527
GGTACCT4550.047.176918
CCAGTAG4300.046.45990826
CTGGTTG4550.046.4372113
CGATTAA256.029784E-446.42209249
TGGTTGA4500.046.3099914
TACCTGG4500.046.27950710
ACCTGGT4600.045.93246511
TTGATCC4550.045.80108617
Produced by FastQC (version 0.11.5)