FastQCFastQC Report
Fri 17 Jun 2016
SRR1526961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708229
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC50280.7099398640835097No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT42550.6007943758304164No Hit
TAAGGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG23670.33421393362881213No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC17950.25344909626688544No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC16440.2321283087814817No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC15630.22069133006414593No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA12670.17889693870203No Hit
TAAGGGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG12170.17183707529626718No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT11990.16929552447019255No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC11270.15912932116589407No Hit
TAAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAAGAAGAA10680.15079868234709395No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTTCAAAGGTCC10260.14486839708625318No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT9270.13088986754284276No Hit
TAAGGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGGA8310.11733492980377815No Hit
TAAGGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8270.11677014073131714No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8250.11648774619508662No Hit
TAAGGGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT7180.10137963850675416No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGGTCC7100.10025006036183212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC150.00462688858.1877955
CCTAACG150.004638604458.15063551
CGTAATC150.00464121158.1423846
CGTACTC202.0030951E-458.03944836
CTATTAC258.711309E-657.94915827
GCGAATA150.00472388157.8836820
AGATACG150.004730488657.86324311
TAAGGGG706450.057.2898941
AAGGGGG694900.057.233832
AGGGGGG663650.056.9544453
TAAGGGA7750.054.1301351
CCGTTAA404.712274E-850.73421530
TTGATCC5600.050.11370517
TAGCATA5500.049.5482230
CATGCAT4950.049.37145658
GTAGCAT5750.048.89960529
TCTATAT302.5491732E-548.33539631
ACCTGGT6050.047.82086611
AGGGGGA21300.047.696263
ATATGCT5550.047.56841734
Produced by FastQC (version 0.11.5)