FastQCFastQC Report
Fri 17 Jun 2016
SRR1526960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705986
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC47400.6714014158920999No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT39640.5614842220667265No Hit
TAAGGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG22410.31742839093126496No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC16910.2395231633488483No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC15540.22011767938740992No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC14800.20963588513086664No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA11850.16785035397302497No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT11550.16360097792307496No Hit
TAAGGGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG11160.15807678905813996No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC10470.14830322414325497No Hit
TAAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAAGAAGAA9870.13980447204335497No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTTCAAAGGTCC9750.13810472162337498No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT8610.12195709263356498No Hit
TAAGGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGGA7950.11260846532367497No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC7440.10538452603875997No Hit
TAAGGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7400.10481794256543332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATA150.004584877858.32195721
TATCGTT150.004776143557.7239
TAAGGGG705650.057.117541
AAGGGGG694450.057.0722662
AGGGGGG666700.056.868193
CATATGC6000.051.13714233
CAGTAGC6350.050.5331327
CATGCAT5750.050.49432458
GTAGCAT6100.050.25242629
ATTAAGC5900.050.16618751
ATATGCT6150.049.89344434
GGTACCT7150.049.309068
TAGCATA6250.049.0463730
TATGCTT6200.049.04508235
CCAGTAG6500.048.9392426
TAAGGGA6550.048.9207231
GCATATG6300.048.6847532
CTACGTT302.4676958E-548.6016321
GTTGATC6950.048.58698316
GTACCTG7200.048.5033579
Produced by FastQC (version 0.11.5)