FastQCFastQC Report
Fri 17 Jun 2016
SRR1526959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635252
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC86641.3638681971878877No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG42550.6698129246346332No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC26140.41149024324205197No Hit
TAAGGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC19800.3116873303822735No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC17970.2828798649984573No Hit
TAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG11250.17709507408083722No Hit
TAAGGGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT11120.17504864211368087No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT9890.15568624734750933No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT9460.14891728007153068No Hit
TAAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAG9360.14734310163525657No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC8270.13018455667986878No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTTC8230.12955488530535914No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC6610.10405319463771857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATA150.00834049750.13408336
CCCGTTA252.0729392E-550.0547532
TAGCGTA252.07974E-550.02704230
ATCGTTA150.00842967449.99936729
ATATCGT150.00846131849.95199227
AATATCG150.00846924349.94016326
CTACGTT252.1248934E-549.8457321
TAAGGGG633450.049.37811
AAGGGGG624100.049.31172
AGGGGGG598200.049.0113453
TAAGGGA6100.045.305041
GATAATA401.5499245E-743.60814320
GTTGATC6450.042.84662616
TTGATCC6450.042.84662617
TATGCTT5900.042.04498735
GCCAGTA6300.042.0098425
GGTACCT6650.041.9423078
AGCGTAT306.0991384E-541.69579731
GCATATG6250.041.64555432
CATATGC6200.041.60407333
Produced by FastQC (version 0.11.5)