FastQCFastQC Report
Fri 17 Jun 2016
SRR1526958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446949
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT224845.03055158418522No Hit
AGTTTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT15100.3378461524692974No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT10530.23559735003322527No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC7380.16511951027969635No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.15147142067663202No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT6760.15124768150281126No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6630.14833907224314183No Hit
AGTTTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG5280.11813428377734371No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT5010.1120933260841841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG150.00467441458.03424550
CGTAGAA202.0031753E-458.03424551
CGATTAA1250.058.02121449
AACGATT1300.058.02121447
ATTATCC150.004680651258.014745
GTCTAAA150.004682731358.0081942
ATTATAC150.004682731358.0081942
TAGTAAC150.004682731358.0081943
GTACGTA303.7615246E-757.98866739
CGACACT150.00470148557.94966532
AGGGTAC49800.057.4188426
TAGGGTA51950.057.3359455
TTAGGGT66850.057.0323264
TAGGGAT27500.056.9566885
TTTAGGG448350.056.8551863
GTTTAGG451650.056.851552
TTAGGGG230150.056.8438074
AGTTTAG454850.056.682071
TAGGGGG115850.056.5638775
ATATGCT43000.056.54729534