FastQCFastQC Report
Fri 17 Jun 2016
SRR1526956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631625
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT499027.900573916485257No Hit
AGTTTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT40320.6383534533940234No Hit
AGTTTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG32900.520878685929151No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT24980.3954878290124679No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC22550.3570156342766673No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC18830.29811992875519494No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT17460.27642984365723333No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC16890.26740550168216903No Hit
AGTTTAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC16110.25505640213734415No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC15450.24460716406095387No Hit
AGTTTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG15020.2377993271323966No Hit
AGTTTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG14210.2249752622204631No Hit
AGTTTAGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT13210.2091430833168415No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC12620.19980209776370472No Hit
AGTTTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC10970.17367900257272909No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT10470.16576291312091826No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.16354640807441123No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10210.16164654660597663No Hit
AGTTTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT9900.15673857114585396No Hit
AGTTTAGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA9160.14502275875717396No Hit
AGTTTAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT8950.14169800118741344No Hit
AGTTTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG8590.13599841678210964No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC8340.13204037205620422No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8280.13109044132198694No Hit
AGTTTAGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGC7740.12254106471403126No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT6760.1070255293884821No Hit
AGTTTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATT6690.10591727686522859No Hit
AGTTTAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT6520.1032258064516129No Hit
AGTTTAGGGAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT6420.10164258856125075No Hit
AGTTTAGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG6390.10116762319414209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTC150.004545466558.44852442
ATAGGTC201.9476513E-458.36890825
ATACGGT150.004642990458.1359957
CGCATAA150.004651799358.10816655
ACGTTAA150.004736105457.8451528
GGCATAA150.00476595557.7534268
GATACGC150.004794440757.6665610
TAGGGTA70800.057.2867055
AGGGTAC67950.057.1386266
TTAGGGT89500.056.7757384
GCCAGTA63650.056.7641125
GGTACCT67150.056.721358
GTTTAGG639300.056.6195372
CCAGTAG63550.056.60056326
TTTAGGG635100.056.5664023
AGTTTAG642450.056.5400051
TTAGGGG342100.056.526574
TAGGGGC54950.056.462045
TTGATCC66050.056.45319717
GTACCTG67500.056.4277959
Produced by FastQC (version 0.11.5)