FastQCFastQC Report
Fri 17 Jun 2016
SRR1526955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573961
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5776710.064621115371951No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC39980.6965630068941966No Hit
AGTTTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC35120.6118882641851973No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC32850.5723385386811995No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC29550.5148433430145951No Hit
AGTTTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC28280.4927164040762351No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT26400.4599615653328362No Hit
AGTTTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC13120.22858695974116708No Hit
AGTTTAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG13040.22719313681591607No Hit
AGTTTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT12580.21917865499572273No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC12210.21273222396643673No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA12190.21238376823512398Illumina Single End Adapter 1 (95% over 21bp)
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA11770.20506619787755614No Hit
AGTTTAGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT11510.20053627337049035No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11270.19635480459473728No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC9980.1738794099250646No Hit
AGTTTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC9920.17283404273112632No Hit
AGTTTAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG9790.17056908047759342No Hit
AGTTTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC9610.16743297889577863No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC9370.1632515101200256No Hit
AGTTTAGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA8460.14739677434529525No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC7790.13572350734631797No Hit
AGTTTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC7180.12509560754127894No Hit
AGTTTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC7090.12352755675037154No Hit
AGTTTAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC6790.11830072078068021No Hit
AGTTTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA6780.11812649291502385No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT6220.10836973243826672No Hit
AGTTTAGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCA6180.1076728209756412No Hit
AGTTTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6160.10732436524432845No Hit
AGTTTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG5880.10244598500594988No Hit
AGTTTAGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT5750.10018102275241697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA150.00799109950.6791449
CAACCGT150.008257695550.26015546
GTACGAC204.1315146E-450.12349735
CGTAGCA301.0519107E-649.96572528
ATACATC355.4002157E-849.8610923
GATACAT355.4151315E-849.84369722
TTTAGCG204.2673683E-449.795923
AGGTATG150.00857505749.78290610
TCGCAAT204.2728754E-449.78290615
GACCGTT150.00857505749.78290613
TTATCGC150.00857505749.78290612
CGTCGCA150.00857505749.78290611
AGGGTAC75700.049.2038966
TTAGGGT94800.049.1655924
TAGGGTA78350.049.1285865
CATATGC68700.048.94968433
TATGCTT68150.048.90994335
ATATGCT68300.048.8954534
TAGCATA69350.048.8281530
GCATATG69050.048.812432
Produced by FastQC (version 0.11.5)