FastQCFastQC Report
Fri 17 Jun 2016
SRR1526954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80491
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT22272.7667689555354014No Hit
ATTTGCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1810.224869861226721No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT1740.21617323675938924No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1320.16399348995539872No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT1110.13790361655340347No Hit
ATTTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.10932899330359917No Hit
ATTTGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG860.10684424345579009No Hit
ATTTGCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG820.10187474376017194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAG150.00451134158.51255858
GATAATA150.00452258158.4758357
AGATAAT150.00452258158.4758356
CCAGGCA150.00452258158.4758357
CGGGATC1250.058.0748146
CAGGTAC150.004647603758.074816
CGGGTAT150.004647603758.074816
GGTAACA303.7164136E-758.0024878
CAACGAT150.00467060958.00248746
TGGCACG150.00467060958.00248752
CATAGTT150.00467060958.00248745
AAGCATA201.9986558E-458.00248742
TTAAGCA201.9986558E-458.00248752
GAGCATT150.00467060958.00248737
CAAGATA201.9986558E-458.00248754
ATGCAAG201.9986558E-458.00248738
CCTTAGT150.004682142857.96639613
CAGTCGT150.004682142857.96639613
ATAGTAG150.004682142857.96639611
AGCACGA150.004682142857.96639611
Produced by FastQC (version 0.11.5)