FastQCFastQC Report
Fri 17 Jun 2016
SRR1526952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122581
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT63435.1745376526541635No Hit
ATTTGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG7070.5767614883220075No Hit
ATTTGCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT6880.5612615331902987No Hit
ATTTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4300.3507884582439366No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4090.33365692888783743No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3690.3010254444000294No Hit
ATTTGCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG3460.2822623408195397No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3420.27899919237075893No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2650.2161835847317284No Hit
ATTTGCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2560.20884150072197158No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2470.20149941671221477No Hit
ATTTGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2210.18028895179513954No Hit
ATTTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2180.17784159045855394No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2120.1729468677853827No Hit
ATTTGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1920.15663112554147868No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1890.15418376420489308No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1780.14521010597074588No Hit
ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1770.14439431885855067No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1740.14194695752196507No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1710.13949959618537947No Hit
ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1680.13705223484879384No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1570.12807857661464664No Hit
ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1490.12155227971708503No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1480.12073649260488982No Hit
ATTTGCGGGACACTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCTTACCTCGT1470.11992070549269464No Hit
ATTTGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1460.11910491838049943No Hit
ATTTGCGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA1450.11828913126830422No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1400.11421019570732821No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1400.11421019570732821No Hit
ATTTGCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGC1380.11257862148293782No Hit
ATTTGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC1340.109315473034157No Hit
ATTTGCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC1340.109315473034157No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1300.10605232458537621No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG1230.1003418148000098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC150.004544491458.4230341
CCCCAAA150.004544491458.4230326
ACAGTCG150.00455192658.3989325
CACGGAT150.00455192658.3989344
TCCCCAA150.00455192658.3989325
TTACACG150.00455192658.3989325
ATAGGCT201.936438E-458.39892624
GCAACGA406.675691E-1058.39892624
TGGCCTA150.00456682258.35078420
TGAACTA150.004672114758.01598458
GAAAGTG202.0045567E-457.99221854
TAAGAGA150.00467970457.99221850
GATACGT150.004687302757.96846837
CTTGCAT150.004687302757.96846836
AGCGCGT150.004687302757.96846837
GCGATAG150.004687302757.96846836
ATACGTG150.004687302757.96846838
CGATACG150.004687302757.96846836
GCGATAC150.004694910357.94474435
CTGGTAA202.0249501E-457.87367233
Produced by FastQC (version 0.11.5)