FastQCFastQC Report
Fri 17 Jun 2016
SRR1526951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111826
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG69456.210541376781786No Hit
ATTTGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC5900.5276053869404252No Hit
ATTTGCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC5790.5177686763364513No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5270.4712678625722104No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3860.3451791175576342No Hit
ATTTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA3220.2879473467708762No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3200.28615885393379No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3000.2682739255629281No Hit
ATTTGCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC2820.2521774900291524No Hit
ATTTGCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT2610.2333983152397475No Hit
ATTTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2140.19136873356822207No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1880.16811832668610163No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1820.16275284817484306No Hit
ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1820.16275284817484306No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1780.1591758625006707No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG1770.1582816160821276No Hit
ATTTGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1720.1538103839894121No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1670.14933915189669666No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1640.14665641264106738No Hit
ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1610.1439736733854381No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1590.1421851805483519No Hit
ATTTGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1480.13234846994437788No Hit
ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC1460.1305599771072917No Hit
ATTTGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1440.1287714842702055No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1410.1260887450145762No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1380.12340600575894693No Hit
ATTTGCGGGACACTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCT1350.12072326650331765No Hit
ATTTGCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC1330.11893477366623145No Hit
ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1320.11804052724768838No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC1280.114463541573516No Hit
ATTTGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1270.1135692951549729No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA1250.11178080231788672Illumina Single End Adapter 1 (95% over 21bp)
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1200.10730957022517125No Hit
ATTTGCGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT1160.10373258455099887No Hit
ATTTGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1130.10104984529536959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAA150.00799954150.63830650
TATGACC251.9250509E-550.63830650
CTATGAC251.9302435E-550.6152849
AGATTTT150.00801394650.61527649
CCTTGAA150.00801394650.61527648
ATAAGTC150.00826180250.22698647
AATAAGT150.00826180250.22698646
ACCTATG252.0203111E-550.22698247
TTAGACT150.00827655750.2043345
TGAAATA150.00827655750.2043343
CCTAAAC252.0257157E-550.2043342
ATGAAAT150.00827655750.2043342
GATTCTG150.00827655750.2043342
CGCGGTC150.00829133150.18169441
CGATTCT150.00829133150.18169441
TATCATG150.00830612550.1590838
TATGCAA204.102637E-450.1590837
ATCCGAG150.00830612550.1590840
CACTCGC150.00830612550.1590836
ACTCGCG150.00830612550.1590837