##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526951_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 111826 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.4570940568383 39.0 38.0 40.0 36.0 40.0 2 38.44625579024556 39.0 39.0 40.0 36.0 40.0 3 38.53898020138429 39.0 39.0 40.0 36.0 40.0 4 38.477563357358754 39.0 39.0 40.0 36.0 40.0 5 37.95798830325685 39.0 38.0 40.0 35.0 40.0 6 38.21621089907535 39.0 38.0 40.0 35.0 40.0 7 38.637696063527265 39.0 39.0 40.0 38.0 40.0 8 38.599806842773596 39.0 39.0 40.0 38.0 40.0 9 38.62963890329619 39.0 39.0 40.0 38.0 40.0 10 37.7339795754118 39.0 38.0 40.0 35.0 40.0 11 37.41754153774614 39.0 37.0 40.0 33.0 40.0 12 37.431509666803784 39.0 37.0 40.0 33.0 40.0 13 37.38149446461467 39.0 37.0 40.0 33.0 40.0 14 37.56291023554451 39.0 37.0 40.0 33.0 40.0 15 37.50474844848247 39.0 37.0 40.0 33.0 40.0 16 37.418525208806535 39.0 37.0 40.0 33.0 40.0 17 37.1942660919643 39.0 37.0 40.0 33.0 40.0 18 37.194766869958684 39.0 37.0 40.0 33.0 40.0 19 36.96338060916066 38.0 36.0 39.0 33.0 40.0 20 36.882290343927174 38.0 36.0 39.0 33.0 40.0 21 36.791730009121316 38.0 36.0 39.0 32.0 40.0 22 36.69730652978735 38.0 36.0 39.0 32.0 40.0 23 36.60449269400676 38.0 36.0 39.0 31.0 40.0 24 36.34929265108293 38.0 35.0 39.0 31.0 40.0 25 36.16112531969309 38.0 35.0 39.0 31.0 40.0 26 35.73985477438163 38.0 35.0 39.0 30.0 40.0 27 35.58290558546313 38.0 35.0 39.0 29.0 40.0 28 35.07999928460286 38.0 34.0 39.0 28.0 40.0 29 35.014084381092054 38.0 34.0 39.0 28.0 40.0 30 34.5992524099941 38.0 33.0 39.0 27.0 40.0 31 33.847432618532366 37.0 33.0 39.0 25.0 40.0 32 33.73896947042727 37.0 33.0 39.0 24.0 40.0 33 33.400506143472896 36.0 33.0 38.0 23.0 40.0 34 33.69052814193479 37.0 33.0 39.0 24.0 40.0 35 33.3337864181854 37.0 32.0 39.0 23.0 40.0 36 33.10008405916334 37.0 33.0 39.0 19.0 40.0 37 32.704988106522634 36.0 32.0 39.0 17.0 40.0 38 32.547556024538125 36.0 32.0 39.0 16.0 40.0 39 32.35278915457943 36.0 31.0 39.0 13.0 40.0 40 31.949224688355123 36.0 31.0 39.0 9.0 40.0 41 31.5383631713555 36.0 31.0 39.0 4.0 40.0 42 31.203602024573893 36.0 30.0 39.0 4.0 40.0 43 30.90994938565271 35.0 30.0 39.0 4.0 40.0 44 30.845751435265502 36.0 30.0 39.0 4.0 40.0 45 30.30860443903922 35.0 29.0 38.0 4.0 40.0 46 29.65291613757087 35.0 28.0 38.0 4.0 40.0 47 29.24117825908107 35.0 27.0 38.0 4.0 39.0 48 29.027846833473433 35.0 27.0 38.0 4.0 39.0 49 28.7132330585016 34.0 26.0 38.0 4.0 39.0 50 28.224151807272012 34.0 24.0 38.0 4.0 39.0 51 27.16434460679985 33.0 18.0 38.0 4.0 39.0 52 26.840967216926295 33.0 16.0 38.0 4.0 39.0 53 26.663128431670632 33.0 10.0 38.0 4.0 39.0 54 26.062445227406865 33.0 4.0 37.0 4.0 39.0 55 25.692423944342103 33.0 4.0 37.0 4.0 39.0 56 15.292686852789155 19.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.7575859232667739 1 2 -0.7712465924170004 1 3 -1.0929330103940416 1 4 -0.9201624861526412 1 5 -1.567778923761189 1 6 -1.0198841173459385 1 7 -0.5918355176545376 1 8 -0.9147266776008536 1 9 -1.0067083720735397 1 10 -0.9607655592202065 1 11 -1.8060321616489858 1 12 -1.4330364335764187 1 13 -1.377577212925594 1 14 -1.0500310700569457 1 15 -1.3863985016703353 1 16 -0.8599073141920428 1 17 -0.6661170897557867 1 18 -0.9445843337379003 1 19 -0.7640032143755136 1 20 -0.118732881904684 1 21 -0.7409070615636892 1 22 -0.6916078862823767 1 23 -0.9474141878540934 1 24 -0.957538432187512 1 25 -1.393836915055644 1 26 -0.5443144734847607 1 27 -0.9147169275488878 1 28 -0.30720262052907543 1 29 -0.43383454549964995 1 30 -0.7050083259806144 1 31 -0.06967257142424188 1 32 -0.6472090002277113 1 33 -0.7125103217216306 1 34 -0.5315328978512639 1 35 -1.3512488236766238 1 36 -0.09791902487248905 1 37 0.49761637671986847 1 38 -0.45005484583546007 1 39 -0.28454640221413285 1 40 0.4429469752173496 1 41 0.3169470444208571 1 42 0.18845278540751664 1 43 0.424055415651992 1 44 -0.6274564108520373 1 45 -0.07960027760275779 1 46 1.051374562043378 1 47 1.2340508405978596 1 48 0.8931048076137955 1 49 1.858824936875088 1 50 1.412475662954769 1 51 2.0797304558429808 1 52 2.5476627159579373 1 53 1.734653026763329 1 54 1.6747462508579716 1 55 2.135370329624312 1 56 2.2873740069265143 2 1 -0.4319847917250428 2 2 -0.2829863378201125 2 3 -0.17694998351992552 2 4 -0.1409074177886609 2 5 -0.7447709086739636 2 6 -0.4640142446443818 2 7 -0.30838431539145716 2 8 -0.6074188039563495 2 9 -0.52608602412446 2 10 -0.8858009198991326 2 11 -0.4096153771133899 2 12 -0.46160786214785077 2 13 0.10738271163829438 2 14 -0.06087501159395714 2 15 -0.26673796418801743 2 16 -0.12978001574791165 2 17 -0.24914396387177362 2 18 -0.6492519433560062 2 19 -0.38107063540332575 2 20 -0.4891242067514554 2 21 -0.5112040912666558 2 22 -0.28859044168077475 2 23 -0.6374660501831855 2 24 -0.801197083766958 2 25 -1.0781367736129255 2 26 -1.0246539360024443 2 27 -0.9183001430132904 2 28 -0.5039022904960717 2 29 -0.17801182036999563 2 30 0.16920195219005763 2 31 0.054042657241481606 2 32 -0.37978797241064655 2 33 0.48428364338916197 2 34 -0.34407415197667746 2 35 0.18661067655911268 2 36 0.08303335607989482 2 37 0.7022368387660762 2 38 0.5572058802371487 2 39 0.05680201834221066 2 40 -0.31160276547100096 2 41 0.7142124852506555 2 42 0.21259234222033996 2 43 0.5742675797255394 2 44 1.0918269460550825 2 45 0.32502018444344927 2 46 0.6016810212607275 2 47 -0.19480347340496706 2 48 -0.7195307416554186 2 49 -0.4926130640678643 2 50 -0.6608859589217069 2 51 -0.39910028437389755 2 52 -0.23357255042584413 2 53 0.23036259772042555 2 54 0.4246755295001208 2 55 -0.1287503681597535 2 56 0.33254138080675943 3 1 -0.04471463613806037 3 2 -0.22669213697145807 3 3 -0.41966568366137125 3 4 -0.28649911981600695 3 5 -0.7756997158403962 3 6 -0.4654286718013836 3 7 -0.3245087849812691 3 8 -0.162251430076104 3 9 -0.1453222334596802 3 10 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0.9273271481032843 3 51 0.7628987726746637 3 52 1.8545934090272453 3 53 0.45742530399105163 3 54 1.6549442706599535 3 55 1.4918059731886686 3 56 -0.24727945842002086 4 1 -0.3764440707637675 4 2 -0.3048821989683077 4 3 -0.6418364988737295 4 4 -0.49275432477575265 4 5 -1.0012017052916775 4 6 0.055344900882985826 4 7 -0.08114800030467251 4 8 0.009581641081481962 4 9 -0.042240897288678525 4 10 -0.5092989172289037 4 11 0.3288803950272481 4 12 0.22116917390021484 4 13 0.20075164799788325 4 14 -0.15477799334740894 4 15 -0.028019673133279355 4 16 -0.07023395433269286 4 17 -0.1644977689452034 4 18 0.21910586705787694 4 19 0.3183508154199899 4 20 0.37344811189562677 4 21 -0.6084003529488982 4 22 0.4156374176872717 4 23 -0.15006062072168191 4 24 0.3567023376838705 4 25 0.3512357230492853 4 26 0.8794849157999636 4 27 0.9304760029591179 4 28 0.3939170241456722 4 29 0.7978671293407302 4 30 1.184600261046306 4 31 1.2619643305837158 4 32 1.8492284939889956 4 33 0.9089387390722976 4 34 0.6976703963099311 4 35 1.6367219360161656 4 36 0.2572211620434004 4 37 0.7146978979561069 4 38 0.6494177182433774 4 39 1.1992141233711422 4 40 1.5565770298115957 4 41 1.822267669941354 4 42 1.3828638152955541 4 43 1.2760032272555826 4 44 1.066815820109376 4 45 0.6795373183998343 4 46 2.1698163127605063 4 47 1.7242886494254712 4 48 1.4913281813530368 4 49 1.7574091878235478 4 50 2.005424677482388 4 51 1.9076197868321536 4 52 1.9444425808603185 4 53 1.5437582363497064 4 54 2.1851561703545954 4 55 1.2465945361571151 4 56 1.5454474778250056 5 1 -0.2181319778722326 5 2 -0.09077854561231646 5 3 -0.305954312524257 5 4 -0.05057552745677185 5 5 0.2786057147026568 5 6 -0.057087837717972434 5 7 0.005324198317055107 5 8 0.14957975304221094 5 9 0.278820180781743 5 10 0.08533905124084384 5 11 -0.10716227466028272 5 12 -0.5239455244855122 5 13 0.1480753523309346 5 14 -0.5644825152014619 5 15 -0.01046090791096077 5 16 -0.8380051239730193 5 17 -0.8165321312599403 5 18 -1.5254424195464864 5 19 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1.542867296840111 6 44 1.2933356683558799 6 45 0.3956472471497179 6 46 0.9622656511522898 6 47 0.5409815336671677 6 48 1.30894639177221 6 49 1.0964486992513258 6 50 0.8580202174102141 6 51 0.5054730301004042 6 52 1.1021181615024922 6 53 0.2396035218128354 6 54 -0.14703592735984827 6 55 -0.4982930367123224 6 56 -0.9898537158457632 7 1 -0.1240177587580149 7 2 -0.08903029386407013 7 3 0.07662144505150081 7 4 -0.14740924929049015 7 5 -0.4638185277215854 7 6 0.2640077333775963 7 7 0.0987585417514012 7 8 0.16172039018285034 7 9 0.061551338512906284 7 10 -0.012174546272753162 7 11 0.013369970871963233 7 12 0.21119433565434775 7 13 0.23723619149177466 7 14 0.42493084921190416 7 15 0.456613684163635 7 16 -0.26137342234132177 7 17 -0.1532648429926482 7 18 -0.4036475477516106 7 19 -0.2793307086634016 7 20 0.1488794562522031 7 21 0.3657189856564216 7 22 0.1760615729712427 7 23 -0.15742942014655625 7 24 0.4733450407751718 7 25 -0.3182466637228174 7 26 1.5486977123492096 7 27 0.7123775126643679 7 28 1.4236618120680262 7 29 0.6949735276153532 7 30 1.5122422452303468 7 31 2.120159873358702 7 32 0.8725929799703067 7 33 1.232177416282937 7 34 1.12964379674127 7 35 1.1425630575114951 7 36 1.1424655905121242 7 37 1.1958265823558207 7 38 0.8078469058781721 7 39 0.8970034835436778 7 40 0.6264008975326583 7 41 1.6778571738953403 7 42 1.860577690205691 7 43 1.5402016456596037 7 44 2.0133470925752306 7 45 1.012840697263961 7 46 1.48730373188344 7 47 1.5727789441774505 7 48 2.2152677931431164 7 49 1.6335774121226123 7 50 2.1598100117742653 7 51 1.6450389097652938 7 52 2.155621588768298 7 53 2.304721572079398 7 54 1.2161590459836376 7 55 1.2598943204849355 7 56 0.924940648206027 8 1 -0.4504552101954644 8 2 -0.5352229900567664 8 3 -0.0029240094939524397 8 4 -0.31320179008303484 8 5 -0.5395761034791633 8 6 -0.12779490842504515 8 7 -0.07548388249102089 8 8 -0.24435964089717999 8 9 -0.53936163740007 8 10 0.42877339467518283 8 11 -0.17786934536735544 8 12 0.2639332633932767 8 13 0.057166261421841114 8 14 0.2840023332833894 8 15 0.32287242542237493 8 16 0.10138881542091838 8 17 0.5875082727804681 8 18 0.5149616208575551 8 19 0.07838102404833336 8 20 -0.0738283914556419 8 21 0.9958666158040472 8 22 0.6300242769339448 8 23 0.9437749310577956 8 24 0.5454118628419877 8 25 1.3478372523610958 8 26 1.4477847364362333 8 27 1.4912236665105212 8 28 2.1001381135443324 8 29 2.2234311810730034 8 30 2.513213496201601 8 31 2.7334077366065657 8 32 3.0291586365359606 8 33 2.915452474557995 8 34 3.0963298884273627 8 35 3.6968559868044224 8 36 2.4980405710871096 8 37 3.591125727654962 8 38 2.975387698418963 8 39 2.9485769101171044 8 40 3.1663193124510762 8 41 3.000215371253539 8 42 2.8928232224512236 8 43 2.753488358946317 8 44 2.59716605139419 8 45 2.9027979622212285 8 46 4.193888813468519 8 47 2.9059180273165346 8 48 4.172532750408077 8 49 3.9694359979812006 8 50 4.471581573545826 8 51 3.9195144342408206 8 52 5.87609555924227 8 53 4.658645426003254 8 54 4.595238300062892 8 55 3.836040396631013 8 56 3.692714540979919 9 1 -0.14627050601075808 9 2 -0.1591768140105856 9 3 -0.1674261739961196 9 4 0.02099734411610399 9 5 0.5028481389450832 9 6 -0.05449043512057017 9 7 -0.0036224106295534853 9 8 0.13543833890079782 9 9 0.016771118732684442 9 10 0.33324669914848926 9 11 0.06657509907709169 9 12 -0.11875071929070202 9 13 0.6883350925906768 9 14 0.3010297503108035 9 15 0.07611917866912421 9 16 0.165458079490179 9 17 0.5222846075567986 9 18 0.3507480566439938 9 19 0.3427388884061955 9 20 0.8251615075342542 9 21 0.8030816230190538 9 22 0.17807622498589382 9 23 1.3244387117215766 9 24 1.0845172019473281 9 25 0.8932917978156425 9 26 1.2705841592356535 9 27 1.2150331903200424 9 28 0.9151453285515458 9 29 1.4791310367728627 9 30 0.7311067140948211 9 31 1.758804562003391 9 32 1.9916405990179271 9 33 1.8176169767224977 9 34 1.6368782289757036 9 35 1.5294678194162543 9 36 2.5116047846513183 9 37 2.1308082673374997 9 38 1.8143487373800014 9 39 1.8472782088184019 9 40 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0.10218969900736852 120 39 0.7535195403024559 120 40 0.5340745345752289 120 41 1.2233202060088573 120 42 1.0162909090257024 120 43 1.3015907420001618 120 44 0.9818870477656674 120 45 1.4712985015955518 120 46 1.6231327087318341 120 47 1.2775959718100118 120 48 0.42064494026783805 120 49 1.4709560528993109 120 50 1.685762214910934 120 51 0.6414914863149583 120 52 2.018392313189505 120 53 0.9339224682705876 120 54 1.722040300651301 120 55 1.1221049254139892 120 56 0.2278719402149889 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 34.0 5 5.0 6 18.0 7 23.0 8 45.0 9 52.0 10 54.0 11 82.0 12 132.0 13 172.0 14 293.0 15 378.0 16 488.0 17 629.0 18 802.0 19 922.0 20 992.0 21 1193.0 22 1164.0 23 1364.0 24 1756.0 25 2269.0 26 2190.0 27 2205.0 28 2507.0 29 2741.0 30 3228.0 31 3753.0 32 4458.0 33 5538.0 34 7203.0 35 9868.0 36 13442.0 37 17285.0 38 16660.0 39 7881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.07534920322644 0.8280721835709048 0.04918355301987016 0.04739506018278397 8 99.66096843159882 0.26299546467004803 0.03399261107980212 0.04204349265133421 9 99.32931518609269 0.3711122636953839 0.09300162752848175 0.20657092268345467 10 63.0318530574285 18.305224187577128 11.586750845062866 7.0761719099315 11 40.50131454223526 30.72362420188507 13.106075510167582 15.668985745712089 12 33.27759197324415 13.799116484538478 21.04340672115608 31.879884821061292 13 25.862500670684813 14.118362455958364 25.047842183392056 34.971294689964765 14 24.14733603991916 15.711909573802158 36.1257668163039 24.01498756997478 15 32.35920090140039 17.422602972475097 26.63334108346896 23.584855042655555 16 38.38731600879938 15.887181871836603 21.97074025718527 23.754761862178743 17 30.47502369753009 16.892314846279042 28.9977286140969 23.634932842093967 18 29.652316992470446 17.3707366801996 28.83765850517769 24.139287822152273 19 36.926117360899966 19.373848657736126 19.52050507037719 24.17952891098671 20 29.183359565030763 27.23565603090571 20.88371011589641 22.69727428816712 21 28.508575823153826 18.971437769391734 30.13342156564663 22.386564841807807 22 26.962423765492822 17.00409564859693 24.030189759090014 32.00329082682024 23 28.283225725680968 18.516266342353298 22.25689911111906 30.943608820846674 24 28.764207721130713 19.102510217039427 27.98350965364907 24.149772408180787 25 36.0763513090457 16.942012003685186 20.23542249930679 26.746214187962323 26 25.92784796535557 17.16384524533839 22.485773594359543 34.42253319494649 27 25.829426291113418 16.501735800436634 21.75566371998139 35.91317418846856 28 26.62382414277659 25.985661478425087 20.013067566479005 27.377446812319313 29 36.01776886150319 19.8726445511213 19.688149315755535 24.421437271619975 30 28.051272857655075 16.590175690211545 29.632484761563283 25.7260666905701 31 28.81612813545338 24.531195350964513 22.853273785502257 23.79940272807985 32 41.37191950353338 16.88937116619435 19.624600925494136 22.114108404778133 33 31.9395113550748 17.44340401105012 21.120797904782407 29.49628672909267 34 27.2240364602024 26.03441469891624 25.620828249479654 21.12072059140171 35 26.905056815121974 22.309584073814783 31.620801694580575 19.16455741648267 36 28.98791459407043 30.0689568480974 20.19322283477293 20.749905723059243 37 29.102762113429836 21.319277324808734 27.474767429330843 22.103193132430587 38 36.98181001571974 22.37951942510667 19.346957107567935 21.29171345160566 39 30.791770405457637 19.530456510601578 19.395633533170948 30.282139550769838 40 28.185939411383753 19.044577966467582 27.904403828164355 24.865078793984313 41 26.621022231419083 19.00062078831119 28.159497611314542 26.21885936895519 42 31.586761688802508 17.72576755569953 22.64874203649714 28.038728719000826 43 23.57749927974647 16.58653846153846 33.02182368193604 26.81413857677903 44 26.588580585915693 16.540324468372436 22.744296595062753 34.12679835064912 45 24.696494830505422 18.126553550199937 30.609892287186135 26.567059332108506 46 24.377477477477477 18.5036036036036 23.440540540540542 33.678378378378376 47 24.5720775533382 24.557655733124218 26.4604346376067 24.409832075930886 48 26.513423091143558 24.14533190249885 24.78560027414308 24.555644732214517 49 27.134396437489293 24.5373333453526 23.35553892890303 24.972731288255073 50 31.883535078382913 17.93426298414302 23.816138401313296 26.366063536160773 51 24.991433260591194 26.418922574710983 23.54861399173986 25.041030172957964 52 25.50015333826421 19.1059477206718 30.816481157433294 24.57741778363069 53 27.247634425090876 16.711016498137308 30.995571030389407 25.045778046382406 54 26.536157991838667 24.649416982486756 22.97897865147095 25.835446374203634 55 26.51899305242263 24.39411711630425 22.234954434719842 26.851935396553277 56 31.151830098970596 18.57706083488962 21.372957660071634 28.898151406068152 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 12.0 9 21.0 10 17.5 11 14.0 12 26.5 13 49.5 14 60.0 15 93.0 16 126.0 17 163.5 18 201.0 19 209.0 20 217.0 21 261.5 22 344.5 23 383.0 24 506.0 25 629.0 26 772.5 27 916.0 28 1021.5 29 1181.0 30 1235.0 31 1057.5 32 880.0 33 878.5 34 877.0 35 805.0 36 733.0 37 746.0 38 830.0 39 901.0 40 1062.5 41 1224.0 42 1764.0 43 2304.0 44 6252.5 45 10201.0 46 6485.0 47 2933.0 48 3097.0 49 3636.5 50 4176.0 51 4155.5 52 4135.0 53 4705.5 54 5799.0 55 6322.0 56 7248.0 57 8174.0 58 9075.5 59 9977.0 60 10003.0 61 10029.0 62 9262.0 63 7741.5 64 6988.0 65 6057.0 66 5126.0 67 4682.5 68 4239.0 69 3631.5 70 3024.0 71 2763.0 72 2160.5 73 1819.0 74 1416.5 75 1014.0 76 835.0 77 656.0 78 549.5 79 330.5 80 218.0 81 166.5 82 115.0 83 78.0 84 41.0 85 31.0 86 21.0 87 15.5 88 5.5 89 1.0 90 1.5 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03308711748609447 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0017884928370861875 21 0.0 22 0.0 23 0.0 24 0.002682739255629281 25 0.02414465330066353 26 0.05544327794967181 27 0.05544327794967181 28 0.08853039543576628 29 0.15202189115232592 30 0.2378695473324629 31 0.2852646075152469 32 0.2843703610967038 33 0.29867830379339333 34 0.3237172035125999 35 0.36842952443975463 36 0.40419938118147836 37 0.41314184536690934 38 0.44801745569008994 39 0.5088262121510203 40 0.581260172053011 41 0.6054048253536745 42 0.6206070144689071 43 0.6742617995814927 44 0.6724733067444065 45 0.706454670649044 46 0.7386475417165954 47 0.7896195875735517 48 0.8361204013377926 49 0.7985620517589828 50 0.85847656180137 51 0.8334376620821633 52 0.85847656180137 53 0.8629477938940854 54 1.605172321284853 55 0.8906694328689214 56 0.8808327222649472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 111826.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.716863698960886 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33924352760663 25.45919553592188 2 11.973014442631335 8.792230787115697 3 5.728342141795952 6.309802729240069 4 3.426776102681507 5.032818843560531 5 2.18466109744514 4.010695187165775 6 1.53437735940963 3.3802514620928945 7 0.9985630434253148 2.566487221218679 8 0.7598821208504835 2.232039060683562 9 0.5406853552205363 1.786704344249101 >10 3.1832241408704545 21.09527301343158 >50 0.21919676562994714 5.485307531343337 >100 0.102291823960642 6.12201098134602 >500 0.007306558854331572 1.516641925849087 >1k 0.0 0.0 >5k 0.002435519618110524 6.210541376781786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 6945 6.210541376781786 No Hit ATTTGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 590 0.5276053869404252 No Hit ATTTGCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 579 0.5177686763364513 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 527 0.4712678625722104 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 386 0.3451791175576342 No Hit ATTTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 322 0.2879473467708762 No Hit ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 320 0.28615885393379 No Hit ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 300 0.2682739255629281 No Hit ATTTGCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 282 0.2521774900291524 No Hit ATTTGCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 261 0.2333983152397475 No Hit ATTTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 214 0.19136873356822207 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 188 0.16811832668610163 No Hit ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 182 0.16275284817484306 No Hit ATTTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 182 0.16275284817484306 No Hit ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 178 0.1591758625006707 No Hit ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG 177 0.1582816160821276 No Hit ATTTGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 172 0.1538103839894121 No Hit ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 167 0.14933915189669666 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 164 0.14665641264106738 No Hit ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 161 0.1439736733854381 No Hit ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 159 0.1421851805483519 No Hit ATTTGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 148 0.13234846994437788 No Hit ATTTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 146 0.1305599771072917 No Hit ATTTGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT 144 0.1287714842702055 No Hit ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 141 0.1260887450145762 No Hit ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 138 0.12340600575894693 No Hit ATTTGCGGGACACTTTTTTTTTTCAAGCTCTTGTAGCCACTTCATGCCCAGTGGCT 135 0.12072326650331765 No Hit ATTTGCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC 133 0.11893477366623145 No Hit ATTTGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 132 0.11804052724768838 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC 128 0.114463541573516 No Hit ATTTGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 127 0.1135692951549729 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 125 0.11178080231788672 Illumina Single End Adapter 1 (95% over 21bp) ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 120 0.10730957022517125 No Hit ATTTGCGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT 116 0.10373258455099887 No Hit ATTTGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 113 0.10104984529536959 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 8.942464185430938E-4 0.0 0.0 0.0 0.0 11 8.942464185430938E-4 0.0 0.0 0.0 0.0 12 8.942464185430938E-4 0.0 0.0 0.0 0.0 13 0.003576985674172375 0.0 0.0 0.0 0.0 14 0.008048217766887843 0.0 0.0 0.0 0.0 15 0.008942464185430937 0.0 0.0 0.0 0.0 16 0.010730957022517125 0.0 0.0 0.0 0.0 17 0.011625203441060218 0.0 0.0 0.0 0.0 18 0.0143079426966895 0.0 0.0 0.0 0.0 19 0.015202189115232594 0.0 0.0 0.0 0.0 20 0.01699068195231878 0.0 0.0 0.0 0.0 21 0.01699068195231878 0.0 0.0 0.0 0.0 22 0.02146191404503425 0.0 0.0 0.0 0.0 23 0.023250406882120437 0.0 0.0 0.0 0.0 24 0.025038899719206625 0.0 0.0 0.0 0.0 25 0.02593314613774972 0.0 0.0 0.0 0.0 26 0.026827392556292812 0.0 0.0 0.0 0.0 27 0.026827392556292812 0.0 0.0 0.0 0.0 28 0.03219287106755137 0.0 0.0 0.0 0.0 29 0.03308711748609447 0.0 0.0 0.0 0.0 30 0.03487561032318066 0.0 0.0 0.0 0.0 31 0.036664103160266845 0.0 0.0 0.0 0.0 32 0.03934684241589612 0.0 0.0 0.0 0.0 33 0.04113533525298231 0.0 0.0 0.0 0.0 34 0.043818074508611596 0.0 0.0 0.0 0.0 35 0.046500813764240874 0.0 0.0 0.0 0.0 36 0.04828930660132706 0.0 0.0 0.0 0.0 37 0.05097204585695634 0.0 0.0 0.0 0.0 38 0.05544327794967181 0.0 0.0 0.0 0.0 39 0.0563375243682149 0.0 0.0 0.0 0.0 40 0.057231770786758 0.0 0.0 0.0 0.0 41 0.060808756460930376 0.0 0.0 0.0 0.0 42 0.06259724929801656 0.0 0.0 0.0 0.0 43 0.06259724929801656 0.0 0.0 0.0 0.0 44 0.06617423497218894 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACAA 15 0.007999541 50.638306 50 TATGACC 25 1.9250509E-5 50.638306 50 CTATGAC 25 1.9302435E-5 50.61528 49 AGATTTT 15 0.008013946 50.615276 49 CCTTGAA 15 0.008013946 50.615276 48 ATAAGTC 15 0.008261802 50.226986 47 AATAAGT 15 0.008261802 50.226986 46 ACCTATG 25 2.0203111E-5 50.226982 47 TTAGACT 15 0.008276557 50.20433 45 TGAAATA 15 0.008276557 50.20433 43 CCTAAAC 25 2.0257157E-5 50.20433 42 ATGAAAT 15 0.008276557 50.20433 42 GATTCTG 15 0.008276557 50.20433 42 CGCGGTC 15 0.008291331 50.181694 41 CGATTCT 15 0.008291331 50.181694 41 TATCATG 15 0.008306125 50.15908 38 TATGCAA 20 4.102637E-4 50.15908 37 ATCCGAG 15 0.008306125 50.15908 40 CACTCGC 15 0.008306125 50.15908 36 ACTCGCG 15 0.008306125 50.15908 37 >>END_MODULE