FastQCFastQC Report
Fri 17 Jun 2016
SRR1526950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174399
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.5722509876776817No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6740.3864701059065706No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.23566648891335384No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.20871679310087787No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.11984013669803152No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.11582635221532234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGG150.004486754558.62256257
GTATCTC150.0046384958.1323551
ACATCTA258.522808E-658.1323551
ACATAGG201.9859533E-458.1155973
ATCACAT201.9944616E-458.06538448
ACTAACC150.00465971558.0653849
CCGCTAA150.00468101257.9985639
TACGTAG150.00468101257.9985640
CAATTGA150.00468634857.9818835
CAGTCCG150.00469168857.9652134
TCGATCT202.0115652E-457.96520632
CGATCTT202.0115652E-457.96520633
AGTTTAA150.00470238257.93189630
GACGTAT202.0259073E-457.88199628
TAGCGCT150.00472919657.84877824
CTCGACA150.00472919657.84877825
TATGACC150.004734572557.83218813
TACGCGT150.004734572557.83218815
CGTAGAA150.004734572557.83218816
TGTCGTA150.004734572557.83218813
Produced by FastQC (version 0.11.5)