FastQCFastQC Report
Fri 17 Jun 2016
SRR1526947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217909
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19380.8893620731589792No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG18650.8558618505890073No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC10360.4754278161985048No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8310.38135184870748795No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8110.3721737055376327No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG7910.36299556236777736No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5840.2680017805597749No Hit
GGACATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5100.23404265083131032No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.19916570678586015No Hit
GGACATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT4290.19687117099339632No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3540.1624531341064389No Hit
GGACATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3160.14501466208371386No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG3080.14134340481577173No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2880.1321652616459164No Hit
GGACATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2870.13170635448742365No Hit
GGACATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2820.12941181869495982No Hit
GGACATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2810.12895291153646704No Hit
GGACATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2710.1243638399515394No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.12023367552510451No Hit
GGACATGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT2520.11564460394017688No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2430.11151443951374197No Hit
GGACATGGGATCTCAGTGGGGTCCAGTTTATATCCAGGTCCAGGACTGGGGCATGG2310.1060075536118288No Hit
GGACATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG2260.10371301781936496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCTG150.00800326850.64885750
ATCACAT251.9342091E-550.62523348
CTCGTCT150.00826481250.238647
TAATAGG150.00827994750.2153644
GATAATA150.00830268850.18053442
GTTAGAT150.00830268850.18053443
AATCTCC150.00830268850.18053442
ACCGTCT150.00830268850.18053443
TCATATC252.0406853E-550.1689441
AAGTTAG150.00831027950.16893841
ATGAATT150.00831787550.1573539
TGAATTC150.00831787550.1573540
ATCCAAT150.00832547550.1457638
TTGCGAT204.1402839E-450.08791435
TAAAAGC150.00836355850.08790634
CGACAGG204.1544487E-450.05326533
CGATCTT252.0687552E-550.05326533
AGAACTA150.00840176950.0301932
CGCGATT204.1733953E-450.00714530
CATTCCG150.00842475949.99562529
Produced by FastQC (version 0.11.5)