FastQCFastQC Report
Fri 17 Jun 2016
SRR1526946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22741
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4431.9480233938701026No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2781.2224616331735632No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.6508069126247746No Hit
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.4353370564179236No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.3078140802955015No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.26823798425750844No Hit
CGCACCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.22866188821951539No Hit
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.2022778241941867No Hit
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1846884481773009No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.17149641616463657No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1670990721604151No Hit
CGCACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.15390704014775075No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG340.14950969614352932No Hit
CGCACCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.14511235213930787No Hit
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG300.1319203201266435No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.12752297612242205No Hit
CGCACCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.12752297612242205No Hit
CGCACCGGGCTTTTCGGACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.12752297612242205No Hit
CGCACCGGGCTTTTCGGACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.12752297612242205No Hit
CGCACCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.11872828811397916No Hit
CGCACCGGGCTTTTCGGACGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10993360010553627No Hit
CGCACCGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.10553625610131481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCATA150.004482876558.467717
CGGGGTT150.004482876558.467716
CGGGCTG600.058.467716
GGGAGCA150.004482876558.467717
CGGGCAT900.058.467716
CGGGTCC150.004482876558.467716
CGGGTCA150.004482876558.467716
CGGGTAA150.004482876558.467716
CGGGATG850.058.467716
CGGGATA150.004482876558.467716
GGGATGG303.4029836E-758.467717
GGGACAT452.7284841E-1158.467717
GGGAACG303.4029836E-758.467717
GGGCCTA150.004482876558.467717
GGGCCAT303.4029836E-758.467717
ACCGGTC201.8914185E-458.4677054
ACCGGCA201.8914185E-458.4677054
GGGCATG550.058.4677057
GGGCAGA201.8914185E-458.4677057
CGGGGAA258.006733E-658.4677056
Produced by FastQC (version 0.11.5)