SRR1526945_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26367
Sequences flagged as poor quality	0
Sequence length	64
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	694	2.632077976258201	No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	470	1.7825311942959003	No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	160	0.6068191299730724	No Hit
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	140	0.5309667387264383	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	119	0.4513217279174726	No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90	0.3413357606098532	No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85	0.3223726627981947	No Hit
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	83	0.3147874236735313	No Hit
CGCACCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79	0.2996169454242045	No Hit
CGCACCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.21238669549057532	No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.21238669549057532	No Hit
CGCACCGGGCTTTTCGGACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.20859407592824364	No Hit
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.20480145636591193	No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52	0.19721621724124852	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG	45	0.1706678803049266	No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.1554974020555998	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC	40	0.1517047824932681	Illumina Single End Adapter 2 (96% over 31bp)
CGCACCGGGCTTTTCGGACGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.1517047824932681	No Hit
CGCACCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1479121629309364	No Hit
CGCACCGGGCTTTTCGGACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1479121629309364	No Hit
CGCACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1479121629309364	No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG	37	0.14032692380627299	No Hit
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.1289490651192779	No Hit
CGCACCGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCCAAAAAAAAAAAAAAAAAAAA	33	0.1251564455569462	No Hit
CGCACCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.1251564455569462	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	31	0.11757120643228279	No Hit
CGCACCGGGCTTTTCGGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30	0.11377858686995107	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.10240072818295597	No Hit
CGCACCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.10240072818295597	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGG	20	1.9595616E-4	58.087624	53
GTAGAGT	40	6.657501E-10	58.087624	43
CGTAGAG	40	6.657501E-10	58.087624	42
GAGTTTG	40	6.657501E-10	58.087624	46
CTGGCTC	20	1.9595616E-4	58.087624	56
TAGAGTT	40	6.657501E-10	58.087624	44
TCCTGGC	20	1.9595616E-4	58.087624	54
AGTTTGA	40	6.657501E-10	58.087624	47
CCTGGCT	20	1.9595616E-4	58.087624	55
CAGTACG	45	2.910383E-11	58.08762	37
TGATCCT	25	8.361803E-6	58.08762	51
ACGTAGA	45	2.910383E-11	58.08762	41
AGTACGT	45	2.910383E-11	58.08762	38
ACTCAGT	45	2.910383E-11	58.08762	34
TCAGTAC	45	2.910383E-11	58.08762	36
TACGTAG	45	2.910383E-11	58.08762	40
GATCCTG	25	8.361803E-6	58.08762	52
CTCAGTA	45	2.910383E-11	58.08762	35
TTGATCC	25	8.361803E-6	58.08762	50
GACTCAG	45	2.910383E-11	58.08762	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content