FastQCFastQC Report
Fri 17 Jun 2016
SRR1526944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26188
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6652.5393309912937223No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4381.6725217656942113No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.5422330838552009No Hit
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.5307774553230487No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.3322132274324118No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.3322132274324118No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG790.30166488468000613No Hit
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG670.2558423705513976No Hit
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.2558423705513976No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.24820528486329618No Hit
CGCACCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.24820528486329618No Hit
CGCACCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.21001985642278906No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC550.21001985642278906Illumina Single End Adapter 2 (96% over 31bp)
CGCACCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.1871085993584848No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG470.1794715136703834No Hit
CGCACCGGGCTTTTCGGACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.1794715136703834No Hit
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.17565297082633266No Hit
CGCACCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.16037879945012984No Hit
CGCACCGGGCTTTTCGGACGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.15656025660607914No Hit
CGCACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1489231709179777No Hit
CGCACCGGGCTTTTCGGACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.14128608522987626No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG370.14128608522987626No Hit
CGCACCGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.12601191385367344No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.11073774247747059No Hit
CGCACCGGGCTTTTCGGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.11073774247747059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGG150.004462508558.56007826
TTTACGG150.004462508558.56007823
TTGCCCG150.004462508558.56007825
CTTTACG150.004496947458.44680822
CATTTTG150.004496947458.44680821
CCTGAGC201.9002E-458.44680820
TCATTTT201.9002E-458.44680820
ATTTTGC150.004496947458.44680822
AGGGGAT150.004531583758.33397740
CAGCAGA150.004531583758.33397742
GCAGCAG150.004531583758.33397741
TAGAGTT150.004531583758.33397744
GGCAGCA150.004531583758.33397740
GGAGATG351.583794E-857.8869748
CAGCCAC150.0046721257.88697434
ATCCTGG150.0046721257.88697453
CGTTCAC150.0046721257.88697431
ACCGGTA150.0046721257.8869744
GGATGGC150.0046721257.8869748
CCGGAGT150.0046721257.8869745
Produced by FastQC (version 0.11.5)