FastQCFastQC Report
Fri 17 Jun 2016
SRR1526943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21932
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7023.2008024803939445No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4752.165785154112712No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.6018602954586905No Hit
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.4605143169797556No Hit
CGCACCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG950.4331570308225424No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.3647638154295094No Hit
CGCACCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.31460879080795184No Hit
CGCACCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG660.30093014772934523No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.2826919569578698No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.27813240926500093No Hit
CGCACCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT600.27357286157213206No Hit
CGCACCGGGCTTTTCGGACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.23709648002918113No Hit
CGCACCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.21885828925770562No Hit
CGCACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.20973919387196788No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA450.20517964617909903No Hit
CGCACCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.18694145540762355No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.18694145540762355No Hit
CGCACCGGGCTTTTCGGACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1823819077147547No Hit
CGCACCGGGCTTTTCGGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1687032646361481No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG370.1687032646361481Illumina Single End Adapter 2 (95% over 23bp)
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.16414371694327923No Hit
CGCACCGGGCTTTTCGGACGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.15046507386467262No Hit
CGCACCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1413459784789349No Hit
CGCACCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC280.1276673354003283No Hit
CGCACCGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1276673354003283No Hit
CGCACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11854824001459056No Hit
CGCACCGGGCTTTTCGGACGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11854824001459056No Hit
CGCACCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT240.10942914462885281No Hit
CGCACCGGGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10486959693598394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT251.8689438E-550.6581448
TTGATCA150.00790051350.6581450
TTTGATC251.8689438E-550.6581449
ACGTAGA309.592059E-750.3071641
CGTAGAG309.592059E-750.3071642
TACGTAG309.592059E-750.3071640
TAGAGTT309.592059E-750.3071644
GTACGTA309.592059E-750.3071639
TGTTCGG150.00819606650.19124635
AGTACGT309.746964E-750.19124638
TCAGTAC309.746964E-750.19124636
TCTGAAC150.00819606650.19124636
GTTCGGC150.00819606650.19124636
ACTCAGT309.903997E-750.07586334
ATGTTCG150.00827122350.07586334
AATGTTC150.00827122350.07586333
TCGGTGG204.0628057E-450.0758629
GAATGTT204.0628057E-450.0758632
CGGTGGT204.0628057E-450.0758630
CGACTCA354.915819E-850.0758632
Produced by FastQC (version 0.11.5)