FastQCFastQC Report
Fri 17 Jun 2016
SRR1526942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41807
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.7917334417681249No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.6625684693950774No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1800.43054990791015857No Hit
CGCTTGGGGAATTCTTTTCTTGGTCATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTT1720.41141435644748486No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.368359365656469No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.2702896644102662No Hit
CGCTTGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTAAAATGTAC810.19374745855957137No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.19135551462673714No Hit
CGCTTGGGGGGTTCAGTGTTTTGGAGCTTGTGAATCTGTCTGCTAGCCTTTTCCCAGGTCAGGT740.17700385102973185No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.14590857990288708No Hit
CGCTTGGGGGTGCTCGCTTGGGGGTTCCAGTCCTGACTTCCTTAGTGATGAACAGCAGTATGGA570.1363408041715502No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11242136484320808No Hit
CGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.10763747697753964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATG201.9199868E-458.40821556
AGTATGG201.9199868E-458.40821557
ACTTGAG150.004527956758.4082156
CTGAACC150.004527956758.4082156
TGAACCT150.004527956758.4082157
GGGTACC501.8189894E-1258.1973537
TGGGGTA1050.058.1973535
TTGGGAG303.5883932E-758.1973534
GGATGTA201.9899188E-457.988018
TGTGAGA150.004659493557.9880129
ACAGCAT150.004659493557.9880140
TAGAAAT150.004659493557.9880130
GAGAATC150.004659493557.9880138
TGTGAAA150.004659493557.9880134
ACCGGAA150.004659493557.9880150
TCCTTAG201.9899188E-457.9880139
TAGTGAT201.9899188E-457.9880143
TGCAAGG150.004659493557.9880128
TATAACT150.004659493557.9880152
ACGTACT150.004659493557.9880141
Produced by FastQC (version 0.11.5)