FastQCFastQC Report
Fri 17 Jun 2016
SRR1526941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54143
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4940.9123986480246754No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.8311323716823965No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.7757235469035703No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3720.6870694272574479No Hit
CGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC3010.5559352086142253No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2840.5245368745728903No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2630.48575069722771186No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.4783628539238683No Hit
CGCTTGGGGAATTCTTTTCTTGGTCATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTT1930.3564634394104501No Hit
CGCTTGGGGGGTTCAGTGTTTTGGAGCTTGTGAATCTGTCTGCTAGCCTTTTCCCAGGTCAGGT1590.29366677132778013No Hit
CGCTTGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1240.22902314241914928No Hit
CGCTTGGGGGTGCTCGCTTGGGGGTTCCAGTCCTGACTTCCTTAGTGATGAACAGCAGTATGGA1220.22532922076722753No Hit
CGCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1200.22163529911530577No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.21424745581146223No Hit
CGCTTGGGGACTTCACTAGCACTTGAGATGGAAGCAGGTGGGTCAGAAGCTCCACCTGCAGCTA1130.20870657333357961No Hit
CGCTTGGGGATGTAAACATCTATTTCTAAACTTATATAATGACCTGATTTTCTTTCTTGCAATT960.17730823929224462No Hit
CGCTTGGGGGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGACAGCGAT920.16992039598840109No Hit
CGCTTGGGATCGCCAGCCCTCAATTCACAAAGATGTATAAGCCTCGTGTTCATGTAGGGTGTAG890.16437951351051844No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.1569916702066749No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.1569916702066749No Hit
CGCTTGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTAAAATGTAC820.15145078772879225No Hit
CGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.1496038269028314No Hit
CGCTTGGGGATTGGCCGCCGCCGCTGTCGCTTAGCAAGAAGCCAGCCTTAGTCTTCGCTTTGGG800.1477568660768705No Hit
CGCTTGGGGACTTGCATGCTACTTATTGAGACTTATCAGTCAGTTGAAGATTATTTTCATTATT780.14406294442494874No Hit
CGCTTGGGGGAAAGGCAAGATGTTGAGGCGCAATCTGGATGAGCGAGATGCTCAAACAAAACAA720.13298117946918347No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG710.13113421864322258No Hit
CGCTTGGAGGAGGAAGACGCAGTGGATTTCATCAGTGTGCCTGTACCAGAGTTTGCGGACAGCG690.12744029699130083No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG680.12559333616533994No Hit
CGCTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG680.12559333616533994No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG670.12374637533937906No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.12005245368745729No Hit
CGCTTGGGGTGTCCAGCCCACCTTGTCTCTGTCGCAGCATGGGGAAGGGCTGGGCTATCTGTCC640.11820549286149641No Hit
CGCTTGGGGATTCTTGGCAGGTGGCATTCTAGAAATAAGGCCATGGCTACTATTGTCTCAGCTG640.11820549286149641No Hit
CGCTTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC630.11635853203553553No Hit
CGCTTGGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC590.108970688731692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAA150.00456739258.30410853
GAAATGA150.00456739258.30410857
CTCCGAA150.00456739258.30410857
GTTTAGA150.00456739258.30410852
ATTGTCT351.537228E-858.30410852
ACTTACA150.00456739258.30410851
TTACACA150.00456739258.30410853
CATGCAT501.8189894E-1258.30410458
AACTTGA258.296669E-658.30410455
ATTAAGC501.8189894E-1258.30410451
CCATGCA501.8189894E-1258.30410457
TAACTTG258.296669E-658.30410454
ACTGAAC201.9421922E-458.304155
TGAACCT201.9421922E-458.304157
TATGACT201.9421922E-458.304151
AGCGCGA201.9421922E-458.304152
ACAGCGA201.9421922E-458.304157
GAACCTT201.9421922E-458.304158
GACTGAA201.9421922E-458.304154
CTATGAC201.951181E-458.24976750
Produced by FastQC (version 0.11.5)