FastQCFastQC Report
Fri 17 Jun 2016
SRR1526940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54242
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.8333026068360311No Hit
CGCTTGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4130.7614026031488514No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.7503410641200546No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3690.6802846502710077No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3100.5715128498211718No Hit
CGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC2580.4756461782382655No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2240.41296412374174996No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.38346668633162495No Hit
CGCTTGGGGAATTCTTTTCTTGGTCATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTT1810.33368976070203904No Hit
CGCTTGGGGGGTTCAGTGTTTTGGAGCTTGTGAATCTGTCTGCTAGCCTTTTCCCAGGTCAGGT1350.24888462814792964No Hit
CGCTTGGGGGTGCTCGCTTGGGGGTTCCAGTCCTGACTTCCTTAGTGATGAACAGCAGTATGGA1110.20463847203274216No Hit
CGCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1100.20279488219460937No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.19173334316581248No Hit
CGCTTGGGGACTTCACTAGCACTTGAGATGGAAGCAGGTGGGTCAGAAGCTCCACCTGCAGCTA990.1825153939751484No Hit
CGCTTGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC980.18067180413701558No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.16961026510821872No Hit
CGCTTGGGGATTCTTGGCAGGTGGCATTCTAGAAATAAGGCCATGGCTACTATTGTCTCAGCTG900.16592308543195308No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG860.15854872607942186No Hit
CGCTTGGGATCGCCAGCCCTCAATTCACAAAGATGTATAAGCCTCGTGTTCATGTAGGGTGTAG860.15854872607942186No Hit
CGCTTGGGGGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGACAGCGAT810.1493307768887578No Hit
CGCTTGGGGATGTAAACATCTATTTCTAAACTTATATAATGACCTGATTTTCTTTCTTGCAATT790.14564359721249218No Hit
CGCTTGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTAAAATGTAC770.14195641753622654No Hit
CGCTTGGGGACTTGCATGCTACTTATTGAGACTTATCAGTCAGTTGAAGATTATTTTCATTATT740.1364256480218281No Hit
CGCTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG710.13089487850742967No Hit
CGCTTGGGGGAAAGGCAAGATGTTGAGGCGCAATCTGGATGAGCGAGATGCTCAAACAAAACAA700.12905128866929685No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.12905128866929685No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.12536410899303124No Hit
CGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1198333394786328No Hit
CGCTTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC640.11798974964049999No Hit
CGCTTGGGGTGTCCAGCCCACCTTGTCTCTGTCGCAGCATGGGGAAGGGCTGGGCTATCTGTCC630.11614615980236717No Hit
CGCTTGGAGGAGGAAGACGCAGTGGATTTCATCAGTGTGCCTGTACCAGAGTTTGCGGACAGCG630.11614615980236717No Hit
CGCTTGGGGATTGGCCGCCGCCGCTGTCGCTTAGCAAGAAGCCAGCCTTAGTCTTCGCTTTGGG620.11430256996423437No Hit
CGCTTGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11061539028796874No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG590.10877180044983592No Hit
CGCTTGGGGGCCTGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCTGGCC560.10324103093543749No Hit
CGCTTGGGGATAGGATGAGATGTATCCTGCAAGATGGGAGAATCTTCATTGGCACCTTCAAGGC550.10139744109730468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATT201.9203742E-458.43750423
GATTTCA201.9203742E-458.43750425
GTGGATT201.9203742E-458.43750422
AAAGTAT201.9203742E-458.43750422
CTTGAGA201.9203742E-458.43750422
TTTGCCC201.9203742E-458.43750424
CAGTACC201.9203742E-458.43750425
GTTGAGC150.004526254358.437525
CTATAAT150.004526254358.437522
TTGTCCG150.004526254358.437525
CAACATT150.004526254358.437524
AATATCC150.004526254358.437522
GTAGCAC150.004526254358.437526
TCAGCAC150.004526254358.437522
CTTTGTC150.004526254358.437523
GGTTGAG150.004526254358.437524
TATAATT150.004526254358.437523
TAATATC150.004543016758.38303821
CAGAACA150.004543016758.38303845
TTGGAGC150.004543016758.38303821
Produced by FastQC (version 0.11.5)