FastQCFastQC Report
Fri 17 Jun 2016
SRR1526939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49290
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5171.0488942990464596No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4160.843984581050923No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.6593629539460337No Hit
CGCTTGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA3240.657334144856969No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.5234327449786975No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2570.5214039358896327No Hit
CGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG2170.44025157232704404No Hit
CGCTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1600.32460945425035503No Hit
CGCTTGGGGAATTCTTTTCTTGGTCATGTGTGAGAAAGCACAGCATTGAGTTATAA1370.27794684520186647No Hit
CGCTTGGGGGGTTCAGTGTTTTGGAGCTTGTGAATCTGTCTGCTAGCCTTTTCCCA1320.2678027997565429No Hit
CGCTTGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC1090.22114019070805438No Hit
CGCTTGGGGGAGGACCACCGTGGCAGCTTGAGGGCGGCCTAGAGGCCTGTCCGGGG1080.21911138161898966No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.19882329072834246No Hit
CGCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT970.19679448163927774No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC940.1907080543720836No Hit
CGCTTGGGGGTGCTCGCTTGGGGGTTCCAGTCCTGACTTCCTTAGTGATGAACAGC900.18259281801582472No Hit
CGCTTGGGGACTTGCATGCTACTTATTGAGACTTATCAGTCAGTTGAAGATTATTT890.18056400892676No Hit
CGCTTGGGGACCATCTAAGGTGATGTCATTGTTATCAAAGTGCCCGACACTGAGTC860.17447758165956584No Hit
CGCTTGGGGACTTCACTAGCACTTGAGATGGAAGCAGGTGGGTCAGAAGCTCCACC830.16839115439237168No Hit
CGCTTGGGGATTCTTGGCAGGTGGCATTCTAGAAATAAGGCCATGGCTACTATTGT800.16230472712517752No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC760.15418949076891864No Hit
CGCTTGGGATCGCCAGCCCTCAATTCACAAAGATGTATAAGCCTCGTGTTCATGTA760.15418949076891864No Hit
CGCTTGGGGGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGG750.15216068167985392No Hit
CGCTTGGGGGGCCACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCC740.15013187259078922No Hit
CGCTTGGGGATTGGCCGCCGCCGCTGTCGCTTAGCAAGAAGCCAGCCTTAGTCTTC740.15013187259078922No Hit
CGCTTGGGGTGTCCAGCCCACCTTGTCTCTGTCGCAGCATGGGGAAGGGCTGGGCT730.14810306350172447No Hit
CGCTTGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTA730.14810306350172447No Hit
CGCTTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA730.14810306350172447No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.14404544532359506No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG670.13593020896733618No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.12984378170014202No Hit
CGCTTGGGGATGTAAACATCTATTTCTAAACTTATATAATGACCTGATTTTCTTTC640.12984378170014202No Hit
CGCTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA590.11969973625481842No Hit
CGCTTGGAGGAGGAAGACGCAGTGGATTTCATCAGTGTGCCTGTACCAGAGTTTGC530.10752688172043011No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC530.10752688172043011No Hit
CGCTTGGGGGAAAGGCAAGATGTTGAGGCGCAATCTGGATGAGCGAGATGCTCAAA510.10346926354230068No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.10346926354230068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTATT203.859891E-450.7246449
AGATTAT203.859891E-450.7246448
TACTATT203.859891E-450.7246448
ACTATTG203.859891E-450.7246449
TATACTT150.00791905950.72463650
GCAAGGG150.00791905950.72463649
GTCCGGG150.00791905950.72463649
TTATACT150.00791905950.72463649
GCCAGCG150.00791905950.72463649
AAGCGCC150.00791905950.72463650
GATTAAG451.1277734E-1050.72463650
TAGGGGC150.00791905950.72463650
CCTAGGG150.00791905950.72463648
GCTCAAA150.00791905950.72463650
AGGTGCA204.0001806E-450.35971544
TAGAGTT204.0001806E-450.35971544
CTCAAAG451.2187229E-1050.3597144
GAGGTGC204.0205484E-450.30800643
GTAGAGT204.0205484E-450.30800643
TCGATCC150.00818188350.30800643